Historical database of spontaneous tumors in transgenic rasH2 mice

The Tg.rasH2 mouse is a hemizygous transgenic mouse, approved by regulatory agencies for carcinogenicity assessment. However, the absence of a historical database for the incidence of spontaneous neoplasms has subsequently led to reluctance by some pharmaceutical companies to adopt the use of this short-term carcinogenicity assay. Our laboratory has generated a database summarizing the mortality, body weights, and the incidence of spontaneous tumors in 1420 male and female mice assigned to 26 studies conducted at our facility. In addition, we present the incidence of tumors in positive control mice treated with urethane from these studies. Mortality in the vehicle-treated Tg.rasH2 mouse was low (average of 1% in each study). The most common spontaneous tumors in the Tg.rasH2 mice were alveolar bronchiolar adenoma of the lungs (10.14% in males and 5.77% in females) and hemangiosarcoma of the spleen (3.66% in both males and females). The incidence of all other tumors was generally very low. In the positive control, urethane-treated animals, the incidence of alveolar bronchiolar adenomas and alveolar bronchiolar carcinomas in the lungs was 93.69% and 42.88% in males and 92.43% and 72.79% in females, respectively. In addition, the incidence of splenic hemangiosarcomas in urethane-treated males was 89.18% and 92.25% in females. The 6-month Tg.rasH2 assay is more precise, faster, and more economical than the conventional 2-year mouse assays because of the low incidence of background tumors, very high survival, shorter duration, and the lower number of animals used.

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Using Machine Learning Algorithms on Prediction of Stock Price

Journal of Modeling and Optimization ◽

10.32732/jmo.2020.12.2.84 ◽

2020 ◽

Vol 12 (2) ◽

pp. 84-99

Author(s):

Li-Pang Chen

Keyword(s):

Machine Learning ◽

Stock Price ◽

Short Term Memory ◽

Machine Learning Algorithms ◽

Machine Learning Techniques ◽

Support Vector ◽

Short Term ◽

Learning Techniques ◽

Historical Database ◽

Long Short Term Memory

In this paper, we investigate analysis and prediction of the time-dependent data. We focus our attention on four different stocks are selected from Yahoo Finance historical database. To build up models and predict the future stock price, we consider three different machine learning techniques including Long Short-Term Memory (LSTM), Convolutional Neural Networks (CNN) and Support Vector Regression (SVR). By treating close price, open price, daily low, daily high, adjusted close price, and volume of trades as predictors in machine learning methods, it can be shown that the prediction accuracy is improved.

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Effect of Oral Gavage Dosing Regimens in Female Tg rasH2 Transgenic Mice

Toxicologic Pathology ◽

10.1080/01926230490457567 ◽

2004 ◽

Vol 32 (4) ◽

pp. 413-417 ◽

Cited By ~ 1

Author(s):

Daniel Morton ◽

Rani S. Sellers ◽

Sylvia M. Furst ◽

Kristen D. Hawley ◽

Jeffrey R. May

Keyword(s):

Body Weight ◽

Incidental Finding ◽

Sodium Dodecyl ◽

Control Group ◽

Oral Gavage ◽

Once Daily ◽

Dosing Regimens ◽

Rash2 Mice ◽

Daily Water ◽

Cortical Architecture

Female Tg rasH2 (CB6F1/Jic-TgrasH2@Tac) mice were administered water once daily, water twice daily with 8 or 12 hours between doses, 1% sodium dodecyl sulfate in water (1% SDS) once daily, or 1% SDS twice daily with 12 hours between doses by oral gavage at volumes of 10 ml/kg/day for 28 or 29 consecutive days. A control group of mice received no treatment and no sham manipulation. There were no significant differences in body weight or food consumption between treated groups and untreated control mice. Mean weights of spleens, livers, and thymuses were lower than control values in most groups of mice subjected to gavage. Focal or multifocal loss of thymic cortical architecture was observed in 13 of 50 mice distributed among all groups (including naïve controls), however only in one instance was this finding suggestive of a precursor to neoplasia. This study demonstrated that Tg rasH2 mice can tolerate once or twice daily gavage dosing with water or vehicle containing 1% SDS. Loss of thymic cortical architecture was a common incidental finding in female Tg rasH2 mice.

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A 28-day oral gavage toxicity study of 3-monochloropropane-1,2-diol (3-MCPD) in CB6F1-non-Tg rasH2 mice

Food and Chemical Toxicology ◽

10.1016/j.fct.2015.09.019 ◽

2015 ◽

Vol 86 ◽

pp. 95-103 ◽

Cited By ~ 12

Author(s):

Byoung-Seok Lee ◽

Sang-Jin Park ◽

Yong-Bum Kim ◽

Ji-Seok Han ◽

Eun-Ju Jeong ◽

...

Keyword(s):

Toxicity Study ◽

Oral Gavage ◽

Rash2 Mice

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The effect of ‘aging’ of an inhibitor of spontaneous tumors in mice. II. Observations at aging from 101-1346 days

Journal of Surgical Oncology ◽

10.1002/jso.2930030402 ◽

1971 ◽

Vol 3 (4) ◽

pp. 369-378 ◽

Cited By ~ 1

Author(s):

Leonell C. Strong ◽

Henry Matsunaga

Keyword(s):

Spontaneous Tumors

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Deficiency in Poly(ADP-ribose) Polymerase-1 (PARP-1) Accelerates Aging and Spontaneous Carcinogenesis in Mice

Current Gerontology and Geriatrics Research ◽

10.1155/2008/754190 ◽

2008 ◽

Vol 2008 ◽

pp. 1-11 ◽

Cited By ~ 51

Author(s):

Tatiana S. Piskunova ◽

Maria N. Yurova ◽

Anton I. Ovsyannikov ◽

Anna V. Semenchenko ◽

Mark A. Zabezhinski ◽

...

Keyword(s):

Dna Repair ◽

Life Span ◽

Soft Tissues ◽

Telomere Maintenance ◽

Biological Aging ◽

Spontaneous Tumors ◽

Uterine Tumors ◽

Dna Repair Gene ◽

Spontaneous Carcinogenesis ◽

Parp 1

Genetic and biochemical studies have shown that PARP-1 and poly(ADP-ribosyl)ation play an important role in DNA repair, genomic stability, cell death, inflammation, telomere maintenance, and suppressing tumorigenesis, suggesting that the homeostasis of poly(ADP-ribosyl)ation and PARP-1 may also play an important role in aging. Here we show that PARP- mice exhibit a reduction of life span and a significant increase of population aging rate. Analysis of noninvasive parameters, including body weight gain, body temperature, estrous function, behavior, and a number of biochemical indices suggests the acceleration of biological aging in PARP- mice. The incidence of spontaneous tumors in both PARP- and PARP- groups is similar; however, malignant tumors including uterine tumors, lung adenocarcinomas and hepatocellular carcinomas, develop at a significantly higher frequency in PARP- mice than PARP- mice (72% and 49%, resp.; .05). In addition, spontaneous tumors appear earlier in PARP- mice compared to the wild type group. Histopathological studies revealed a wide spectrum of tumors in uterus, ovaries, liver, lungs, mammary gland, soft tissues, and lymphoid organs in both groups of the mice. These results demonstrate that inactivation of DNA repair gene PARP-1 in mice leads to acceleration of aging, shortened life span, and increased spontaneous carcinogenesis.

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