Potential Protective Effect of Nuclear Factor-κB Decoy Oligodeoxynucleotides on Endotoxin-induced Liver Injury

2011 ◽  
Vol 43 (10) ◽  
pp. 3613-3617 ◽  
Author(s):  
J.D. Li ◽  
Y. Peng ◽  
Q. Li ◽  
J.W. Xiao ◽  
J.P. Gong ◽  
...  
Keyword(s):  
Liver Injury ◽  
Protective Effect ◽  
Nuclear Factor Κb ◽  
Nuclear Factor

scholarly journals Ginseng (Panax ginseng Meyer) Oligopeptides Protect Against Binge Drinking-Induced Liver Damage through Inhibiting Oxidative Stress and Inflammation in Rats

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1665 ◽  
Author(s):  
Rui Liu ◽  
Qi-He Chen ◽  
Jin-Wei Ren ◽  
Bin Sun ◽  
Xia-Xia Cai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Liver Injury ◽  
Binge Drinking ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Oxidative Stress Markers ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Stress Markers

Panax ginseng C.A. Meyer (ginseng) is an edible and traditional medicinal herb, which is reported to have a wide range of biological activity and pharmaceutical properties. There were more studies on ginsenoside and polysaccharides, but fewer on ginseng oligopeptides (GOPs), which are small molecule oligopeptides extracted from ginseng. The present study was designed to investigate the effects and underlying mechanism of ginseng oligopeptide (GOPs) on binge drinking-induced alcohol damage in rats. Sprague Dawley rats were randomly assigned to six groups (n = 10), rats in normal control group and alcohol model group was administered distilled water; rats in four GOPs intervention groups (at a dose of 0.0625, 0.125, 0.25, 0.5 g/kg of body weight, respectively) were administered GOPs once a day for 30 days. Experiment rats were intragastrically administered ethanol at a one-time dose of 7 g/kg of body weight after 30 days. The liver injury was measured through traditional liver enzymes, inflammatory cytokines, expression of oxidative stress markers, and histopathological examination. We found that the GOPs treatment could significantly improve serum alanine aminotransferase and aspartate aminotransferase, plasma lipopolysaccharide, and inflammatory cytokine levels, as well as the oxidative stress markers that were altered by alcohol. Moreover, GOPs treatment inhibited the protein expression of toll-like receptor 4, and repressed the inhibitor kappa Bα and nuclear factor-κB p65 in the liver. These findings suggested that GOPs have a significant protective effect on binge drinking-induced liver injury, and the mechanism possibly mediated by the partial inhibition of lipopolysaccharide—toll-like receptor 4-nuclear factor-κB p65 signaling in the liver.

scholarly journals Emodin has a protective effect in cases of severe acute pancreatitis via inhibition of nuclear factor-κB activation resulting in antioxidation

2014 ◽  
Vol 11 (2) ◽  
pp. 1416-1420 ◽  
Author(s):  
WEI-YAN YAO ◽  
YU-FEN ZHOU ◽  
AI-HUA QIAN ◽  
YONG-PING ZHANG ◽  
MIN-MIN QIAO ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Protective Effect ◽  
Severe Acute Pancreatitis ◽  
Nuclear Factor Κb ◽  
Nuclear Factor

Design and Development of Novel 1,3,5-Triazine-Procaine Derivatives as Protective Agent against Myocardial Ischemia/Reperfusion Injury via Inhibitor of Nuclear Factor-κB

Pharmacology ◽  
2019 ◽  
Vol 104 (3-4) ◽  
pp. 126-138
Author(s):  
Zhe Qiang ◽  
Wenli Yu ◽  
Yonghao Yu
Keyword(s):  
Myocardial Ischemia ◽  
Protective Effect ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Ischemia Reperfusion ◽  
Nuclear Factor Κb ◽  
Treated Group ◽  
Nuclear Factor ◽  
Triazine Derivatives ◽  
Myocardial Ischemia Reperfusion

The aim of the present study was to determine the protective effect of novel 1,3,5-triazine-procaine derivatives against myocardial ischemia/reperfusion (I/R) injury. Initially, the experiment has been started by the synthesis of procaine, which later got substituted with diverse 1,3,5-triazine derivatives to furnish the final compounds. The target compounds were tested for nuclear factor-κB (NF-κB) inhibitory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The antioxidant activity of most potent compound 9i was investigated using hydroxyl radical, DPPH, and superoxide anion scavenging assay. Compound 9i was further evaluated for protective effect against myocardial I/R injury on the basis numerous parameters, for example, hemodynamic parameters (left ventricular developed pressure [LVDP], ±dp/dtmax, coronary flow [CF], and heart rate [HR]), myocardial enzymes (creatine kinase and lactate dehydrogenase), thiobarbituric acid reactive substance (TBARS), oxidative stress (super oxide dismutase [SOD], catalase [CAT], glutathione [GSH], and glutathione peroxidise [GPx]), histopathology, western blots analysis for B-cell lymphoma 2 (Bcl-2), Bcl-2-associated x protein (Bax), lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), and NF-κB in cardiac tissues. Compounds showed significant inhibition of NF-ĸB transcriptional activity in LPS-stimulated RAW264.7 cells, revealing compound 9i as a most potent derivative. In vitro results showed efficient reduction of reduced hydroxyl radical, DPPH, and superoxide anion by 9i. The level LVDP, ±dp/dtmax, CF, HR, TBARS, SOD, CAT, GSH, GPx, and damaged cardiac histopathology were completely restored to normal in 9i-treated group, as compared to I/R group. In western blot analysis, the expression of Bax, LOX-1, and NF-ĸB was found to be decreased, while the level of Bcl-2 was found to be increased in 9i-treated group. The procaine-1,3,5-triazine derivatives showed significant cardioprotective action via inhibition of NF-ĸB.

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