scholarly journals Maternal transfer of neutralizing antibodies to B. burgdorferi OspA after oral vaccination of the rodent reservoir

Vaccine ◽  
2021 ◽  
Author(s):  
Kathryn Phillip ◽  
Nisha Nair ◽  
Kamalika Samanta ◽  
Jose F. Azevedo ◽  
Grant D. Brown ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Maternal Transfer ◽  
Oral Vaccination

scholarly journals Maternal transfer of neutralizing antibodies to OspA after oral vaccination of the rodent reservoir

2021 ◽  
Author(s):  
Kathryn O’Connell ◽  
Nisha Nair ◽  
Kamalika Samanta ◽  
Jose F. Azevedo ◽  
Grant D. Brown ◽  
...  
Keyword(s):  
Bayesian Model ◽  
Neutralizing Antibodies ◽  
Antibody Responses ◽  
Maternal Transfer ◽  
Igg Antibodies ◽  
Oral Vaccination ◽  
E Coli ◽  
Antibody Persistence ◽  
Protective Antibodies ◽  
New Evidence

AbstractLyme Disease presents unique challenges for public health efforts. We hypothesized that transfer of protective antibodies between mothers and offspring should occur after oral vaccination of C3H-HeN mice with E. coli overexpressing OspA. We present new evidence for maternal transfer of vaccine induced neutralizing anti-OspA IgG antibodies to mouse pups through ingestion of colostrum. Protective levels of OspA antibodies in pups were present from 2-5 weeks after birth and they persisted in some mice until 9 weeks of age. This was corroborated by detection of neutralizing antibodies in the serum of all pups at 2-3 weeks after birth and in some mice at 9 weeks of age. A clear association was found between robust antibody responses in mothers and the length of antibody persistence in the respective pups using a novel longitudinal Bayesian model. These factors are likely to impact the enzootic cycle of B. burgdorferi when reservoir targeted OspA-based vaccination interventions are implemented.

scholarly journals Clinical Studies of Vaccine Against Smallpox on the Base of Recombinant Vaccinia b7,5S2-S Strain under the Conditions of Double Oral Vaccination

2010 ◽  
pp. 51-55
Author(s):  
I. V. Plyasunov ◽  
A. A. Sergeev ◽  
L. N. Shishkina ◽  
Al. A. Sergeev ◽  
K. A. Titova ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Clinical Manifestation ◽  
Clinical Studies ◽  
Small Dose ◽  
Neutralizing Antibodies ◽  
Protective Level ◽  
Oral Vaccination

Volunteers who had been vaccinated against smallpox in their childhood were orally immunized with Revax-BT vaccine - initially with a small dose, then (in 7, 14, 30, 90 and 180 days) with a large one. Slight reactogenicity was observed after the first vaccination whereas revaccination induced no clinical manifestation. Double immunization with this preparation with 1-2 weeks interval proved to be the most effective method: the protective level of virus-neutralizing antibodies to vaccinia virus (VV) was formed in 90-100 % volunteers in a month after vaccination, and 6 months later this index decreased up to 70 %. The recombinant VV was not registered in the samples of blood, saliva and urine taken from the volunteers after double immunization.

scholarly journals Immunogenicity Studies in Carnivores Using a Rabies Virus Construct with a Site-Directed Deletion in the Phosphoprotein

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Ad Vos ◽  
Karl-Klaus Conzelmann ◽  
Stefan Finke ◽  
Thomas Müller ◽  
Jens Teifke ◽  
...  
Keyword(s):  
Oral Dose ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Oral Vaccination ◽  
P Gene ◽  
Interferon Induction ◽  
Antibody Titres ◽  
A Site

Different approaches have been applied to develop highly attenuated rabies virus vaccines for oral vaccination of mesocarnivores. One prototype vaccine construct is SAD dIND1, which contains a deletion in the P-gene severely limiting the inhibition of type-1 interferon induction. Immunogenicity studies in foxes and skunks were undertaken to investigate whether this highly attenuated vaccine would be more immunogenic than the parental SAD B19 vaccine strain. In foxes, it was demonstrated that SAD dIND1 protected the animals against a rabies infection after a single oral dose, although virus neutralizing antibody titres were lower than in foxes orally vaccinated with the SAD B19 virus as observed in previous experiments. In contrast, skunks receiving 107.5FFU SAD dIND1 did not develop virus neutralizing antibodies and were not protected against a subsequent rabies infection.

Oral vaccination of mice with human papillomavirus virus-like particles induces systemic virus-neutralizing antibodies

Vaccine ◽  
1999 ◽  
Vol 17 (17) ◽  
pp. 2129-2135 ◽  
Author(s):  
Robert C Rose ◽  
Christopher Lane ◽  
Susan Wilson ◽  
JoAnn A Suzich ◽  
Edward Rybicki ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Neutralizing Antibodies ◽  
Oral Vaccination ◽  
Virus Like Particles

Endothelial Cell Protein S Synthesis Is Upregulated by the Complex of IL-6 and Soluble IL-6 Receptor

1997 ◽  
Vol 77 (05) ◽  
pp. 1014-1019 ◽  
Author(s):  
W Craig Hooper ◽  
Donald J Phillips ◽  
Bruce L Evatt
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Line ◽  
Neutralizing Antibodies ◽  
Hepatoma Cell ◽  
Protein S ◽  
Oncostatin M ◽  
Cell Protein ◽  
Microvascular Endothelial Cell ◽  
Hepatoma Cell Line

SummaryWe have recently demonstrated that the proinflammatory cytokine, interleukin-6 (IL-6), could upregulate the production of protein S in the human hepatoma cell line, HepG-2, but not in endothelial cells. In this study, we have demonstrated that the combination of exogenous IL-6 and soluble IL-6 receptor (sIL-6R) could significantly upregulate protein S production in both primary human umbilical vein endothelial cells (HUVEC) and in the immortalized human microvascular endothelial cell line, HMEC-1. The IL-6/sIL-6R complex was also able to rapidly induce tyrosine phosphorylation of the IL-6 transducer, gpl30. Neutralizing antibodies directed against either IL-6 or gpl30 blocked protein S upregulation by the IL-6/sIL-6R complex. It was also observed that exogenous sIL-6R could also upregulate protein S by forming a complex with IL-6 constitutively produced by the endothelial cell. Two other cytokines which also utilize the gpl30 receptor, oncostatin M (OSM) and leukemia inhibitory factor (LIF), were also able to upregulate endothelial cell protein S. This study demonstrates a mechanism that allows endothelial cells to respond to IL-6 and also illustrates the potential importance of circulating soluble receptors in the regulation of the anticoagulation pathway.

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