Exploratory study of humoral and cellular immunity to 17DD Yellow Fever vaccination in children and adults residents of areas without circulation of Yellow Fever Virus

Abstract Monitoring the frequency of circulatory CXCR5+ (cCXCR5+) CD4+ T cells in periphery blood provides a potential biomarker to draw inferences about T follicular helper (TFH) activity within germinal center. However, cCXCR5+ T cells are highly heterogeneous in their expression of ICOS, PD1 and CD38 and the relationship between different cCXCR5 subsets as delineated by these markers remains unclear. We applied class II tetramer reagents and mass cytometry to investigate the ontogeny of different subsets of cCXCR5+ T cell following yellow fever immunization. Through unsupervised analyses of mass cytometry data, we show yellow fever virus-specific cCXCR5 T cells elicited by vaccination were initially CD38+ICOS+PD1+, but then transitioned to become CD38+ICOS−PD1+ and CD38−ICOS−PD1+ before coming to rest as a CD38−ICOS−PD1− subset. These results imply that most antigen-specific cCXCR5+ T cells, including the CD38−ICOS−PD1− CXCR5+ T cells are derived from the CXCR5+CD38+ICOS+PD1+ subset, the subset that most resembles preTFH/TFH in the germinal center.

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Detection of Anti-Yellow Fever Virus Immunoglobulin M Antibodies at 3–4 Years Following Yellow Fever Vaccination

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2012.12-0182 ◽

2012 ◽

Vol 87 (6) ◽

pp. 1112-1115 ◽

Cited By ~ 15

Author(s):

Katherine B. Gibney ◽

Olga I. Kosoy ◽

Marc Fischer ◽

Srilatha Edupuganti ◽

Robert S. Lanciotti ◽

...

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Immunoglobulin M ◽

Fever Virus ◽

Yellow Fever Vaccination

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Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination

Vaccines ◽

10.3390/vaccines7040206 ◽

2019 ◽

Vol 7 (4) ◽

pp. 206

Author(s):

Izabela Maurício de Rezende ◽

Pedro Augusto Alves ◽

Matheus Soares Arruda ◽

Andreza Parreiras Gonçalves ◽

Gabriela Fernanda Garcia Oliveira ◽

...

Keyword(s):

Adverse Events ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus ◽

Wild Type Virus ◽

Wild Type ◽

Relative Safety ◽

Minas Gerais State ◽

Virus Genotypes ◽

Yellow Fever Vaccination

The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 5′UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was efficient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the differentiation of all yellow fever virus genotypes.

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Faculty Opinions recommendation of Detection of anti-yellow fever virus immunoglobulin m antibodies at 3-4 years following yellow fever vaccination.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717961725.793464951 ◽

2012 ◽

Author(s):

Edward Hayes

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Immunoglobulin M ◽

Fever Virus ◽

Yellow Fever Vaccination

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VACCINATION AGAINST YELLOW FEVER WITH IMMUNE SERUM AND VIRUS FIXED FOR MICE

Journal of Experimental Medicine ◽

10.1084/jem.55.6.945 ◽

1932 ◽

Vol 55 (6) ◽

pp. 945-969 ◽

Cited By ~ 25

Author(s):

W. A. Sawyer ◽

S. F. Kitchen ◽

Wray Lloyd

Keyword(s):

Yellow Fever ◽

Room Temperature ◽

Yellow Fever Virus ◽

Single Injection ◽

Immune Serum ◽

Fever Virus ◽

Human Immune ◽

Yellow Fever Vaccination ◽

Observation Period

1. After preliminary experiments in monkeys, 15 persons were actively immunized by a single injection of a dried mixture of living yellow fever virus, fixed for mice, and human immune serum, with separate injections of enough additional serum to make up the amount required for protection. 2. One person was similarly immunized by injecting immune serum and dried virus separately. 3. By titration of the sera of vaccinated persons in mice, it was shown that the immunity rose in a few weeks to a height comparable to that reached after an attack of yellow fever, and remained there throughout an observation period of 6 months. 4. Yellow fever virus could not be recovered from the blood of vaccinated persons or monkeys, except when the latter had received less than the minimal effective amount of immune serum. 5. Neutralization of yellow fever virus by immune serum took place very slowly in vitro at room temperature in our experiments, and could not have been an appreciable factor in vaccination with the serum virus mixtures. 6. A mixture of fixed virus and immune serum retained its immunizing power for 8 months when dried in the frozen state and sealed in glass. 7. It appears that the immunizing reaction after yellow fever vaccination was a part of a true infectious process, as was also the observed leucopenia.

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Yellow fever virus outbreak in Brazil under current and future climate

Infectious Disease Modelling ◽

10.1016/j.idm.2021.04.002 ◽

2021 ◽

Vol 6 ◽

pp. 664-677

Author(s):

Tara Sadeghieh ◽

Jan M. Sargeant ◽

Amy L. Greer ◽

Olaf Berke ◽

Guillaume Dueymes ◽

...

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus ◽

Future Climate

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Process intensification for the production of yellow fever virus‐like particles (VLPs) as potential recombinant vaccine antigen

Biotechnology and Bioengineering ◽

10.1002/bit.27864 ◽

2021 ◽

Author(s):

Renata G. F. Alvim ◽

Túlio M. Lima ◽

Jerson L. Silva ◽

Guilherme A. P. Oliveira ◽

Leda R. Castilho

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Process Intensification ◽

Recombinant Vaccine ◽

Fever Virus ◽

Vaccine Antigen ◽

Virus Like Particles

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Yellow Fever Outbreak in Eastern Senegal, 2020–2021

Viruses ◽

10.3390/v13081475 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1475

Author(s):

Moussa Moïse Diagne ◽

Marie Henriette Dior Ndione ◽

Alioune Gaye ◽

Mamadou Aliou Barry ◽

Diawo Diallo ◽

...

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Mosquito Species ◽

Virus Transmission ◽

Genomic Analysis ◽

Hemorrhagic Fever ◽

Fever Virus ◽

World Health ◽

Effective Vaccine ◽

Ministry Of Health

Yellow fever virus remains a major threat in low resource countries in South America and Africa despite the existence of an effective vaccine. In Senegal and particularly in the eastern part of the country, periodic sylvatic circulation has been demonstrated with varying degrees of impact on populations in perpetual renewal. We report an outbreak that occurred from October 2020 to February 2021 in eastern Senegal, notified and managed through the synergistic effort yellow fever national surveillance implemented by the Senegalese Ministry of Health in collaboration with the World Health Organization, the countrywide 4S network set up by the Ministry of Health, the Institut Pasteur de Dakar, and the surveillance of arboviruses and hemorrhagic fever viruses in human and vector populations implemented since mid 2020 in eastern Senegal. Virological analyses highlighted the implication of sylvatic mosquito species in virus transmission. Genomic analysis showed a close relationship between the circulating strain in eastern Senegal, 2020, and another one from the West African lineage previously detected and sequenced two years ago from an unvaccinated Dutch traveler who visited the Gambia and Senegal before developing signs after returning to Europe. Moreover, genome analysis identified a 6-nucleotide deletion in the variable domain of the 3′UTR with potential impact on the biology of the viral strain that merits further investigations. Integrated surveillance of yellow fever virus but also of other arboviruses of public health interest is crucial in an ecosystem such as eastern Senegal.

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