The influence of fibroblast co-culture and 3D structures on cardiac action potentials of human stem cell-derived cardiomyocytes

2018 ◽  
Vol 93 ◽  
pp. 169
Author(s):  
Maria P. Hortigon-Vinagre ◽  
Victor Zamora ◽  
Gary Gintant ◽  
Jonathon Green ◽  
Francis L. Burton ◽  
...  
Keyword(s):  
Stem Cell ◽  
Action Potentials ◽  
Human Stem Cell ◽  
3D Structures ◽  
Cardiac Action

Abstract 026: Optical Recording of Cardiac Action Potentials from Human Induced Pluripotent Stem Cell Derived Cardiomyocytes

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
B Alexander Yi ◽  
Joel M Kralj ◽  
Adam E Cohen
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
Action Potentials ◽  
Pluripotent Stem Cell ◽  
Gene Mutations ◽  
Induced Pluripotent Stem Cell ◽  
Patch Clamping ◽  
Cardiac Action ◽  
Or Gene ◽  
Induced Pluripotent

The electrically excitable properties of cardiomyocytes stem from the activity of ion channels that allow the coordinated entry of ions to generate cardiac action potentials. Disruptions in ion channel function either by drugs or gene mutations can lead to cardiac arrhythmias. The ability to screen drugs or gene mutations rapidly for effects on the cardiac action potential would be of interest for both drug discovery as well as for studies of ion channel function; however, the time-consuming and technically challenging nature of conventional patch clamping can limit the ability to perform high throughput screens. Archaerhodopsin3, or Arch, is an Archaebacterial variant of the membrane protein bacteriorhodopsin that binds a retinal fluorophore whose signal is rapidly responsive to changes in membrane potential. Here, we report the use of Arch to optically record action potentials from human induced pluripotent stem cell-derived cardiomyocytes. Human induced pluripotent stem cells that stably express Arch were generated and then differentiated into cardiomyocytes. As compared to patch clamping, Arch faithfully reproduces many of the key features of cardiac action potentials and may be a tool to be used for high throughput electrophysiological screens of cardiomyocytes.

scholarly journals Hypertrophic Cardiomyopathy: Prolonged Twitch, Calcium Transients and Action Potentials in Human Stem Cell-Derived Cardiomyocytes with β-Myosin Mutation R723G

2020 ◽  
Vol 118 (3) ◽  
pp. 257a
Author(s):  
Natalie Weber ◽  
Tim Holler ◽  
Joachim Meißner ◽  
Judith Montag ◽  
Martin Fischer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hypertrophic Cardiomyopathy ◽  
Action Potentials ◽  
Calcium Transients ◽  
Human Stem Cell

Human stem cell-derived cardiomyocytes detect drug-mediated changes in action potentials and ion currents

2014 ◽  
Vol 70 (3) ◽  
pp. 255-267 ◽  
Author(s):  
John K. Gibson ◽  
Yimei Yue ◽  
Jared Bronson ◽  
Cassie Palmer ◽  
Randy Numann
Keyword(s):  
Stem Cell ◽  
Action Potentials ◽  
Ion Currents ◽  
Human Stem Cell

New Molecular Scaffolds for Fluorescent Voltage Indicators

2018 ◽  
Author(s):  
Steven Boggess ◽  
Shivaani Gandhi ◽  
Brian Siemons ◽  
Nathaniel Huebsch ◽  
Kevin Healy ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Action Potentials ◽  
Induced Pluripotent Stem Cell ◽  
Molecular Wire ◽  
Voltage Sensing ◽  
Design Synthesis ◽  
Cardiac Action ◽  
Action Potential Waveform ◽  
Induced Pluripotent ◽  
Voltage Sensing Domain

<div> <p>The ability to non-invasively monitor membrane potential dynamics in excitable cells like neurons and cardiomyocytes promises to revolutionize our understanding of the physiology and pathology of the brain and heart. Here, we report the design, synthesis, and application of a new class of fluorescent voltage indicator that makes use of a fluorene-based molecular wire as a voltage sensing domain to provide fast and sensitive measurements of membrane potential in both mammalian neurons and human-derived cardiomyocytes. We show that the best of the new probes, fluorene VoltageFluor 2 (fVF 2) readily reports on action potentials in mammalian neurons, detects perturbations to cardiac action potential waveform in human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes, shows a substantial decrease in phototoxicity compared to existing molecular wire-based indicators, and can monitor cardiac action potentials for extended periods of time. Together, our results demonstrate the generalizability of a molecular wire approach to voltage sensing and highlights the utility of fVF 2 for interrogating membrane potential dynamics.</p> </div>

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