Stability of ceftolozane in human plasma and dried blood spots: Implications for transport and storage

2020 ◽  
Vol 103 ◽  
pp. 106692
Author(s):  
Jens Martens-Lobenhoffer ◽  
Matthias Hinderhofer ◽  
Uwe Tröger ◽  
Stefanie M. Bode-Böger
Keyword(s):  
Human Plasma ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
And Storage

Should phosphatidylethanol be currently analysed using whole blood, dried blood spots or both?

2019 ◽  
Vol 57 (5) ◽  
pp. 617-622 ◽  
Author(s):  
Van Long Nguyen ◽  
Michael Fitzpatrick
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Ethanol Metabolism ◽  
Clinical Use ◽  
Blood Spots ◽  
Comparative Review ◽  
And Storage

Abstract Phosphatidylethanol (PEth) are phospholipids produced through non-oxidative ethanol metabolism. They accumulate in red blood cells and have been traditionally analysed in whole blood as potential biomarkers for moderate to long-term alcohol consumption. More recently, their analysis in dried blood spots has been gaining favour, namely, due to the ease in sampling, transport and storage conditions required. This paper aims at providing a short comparative review between analysing PEth in whole blood and dried blood spots and the potential pitfalls that researchers may face when setting up PEth testing for clinical use.

scholarly journals Nontargeted Mass Spectrometry of Dried Blood Spots for Interrogation of the Human Circulating Metabolome

2021 ◽  
Author(s):  
Casey Ward ◽  
Shriram Nallamshetty ◽  
Jeramie D. Wartrous ◽  
Eowyn Acres ◽  
Tao Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Room Temperature ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Resource Limited ◽  
Metabolomics Study ◽  
Simultaneous Measure ◽  
Analytical Approaches ◽  
And Storage

Advances in high resolution, nontargeted mass spectrometry allow for the simultaneous measure of thousands of metabolites in a single biosample. Application of these analytical approaches to population-scale human studies has been limited by the need for resource-intensive blood sample collection, preparation, and storage. Dried blood spotting, a technique developed decades ago for newborn screening, may offer a simple approach to overcome barriers in human blood acquisition and storage. In this study, we find that over 4,000 spectral features across diverse chemical classes may be efficiently and reproducibly extracted and relatively quantified from human dried blood spots using nontargeted mass spectrometry-based metabolomics. Moreover, over 80% of metabolites were found to be chemically stable in dried blood spots stored at room temperature for up to a week. In direct relation to plasma samples, dried blood spots exhibited comparable representation of the human circulating metabolome, capturing both known and previously uncharacterized metabolites. Dried blood spot approaches provide an opportunity for rapid and facile human biosampling and storage, and will enable widespread metabolomics study of populations, particularly in resource-limited areas.

scholarly journals Nontargeted Mass Spectrometry of Dried Blood Spots for Interrogation of the Human Circulating Metabolome

2021 ◽  
Author(s):  
Casey Ward ◽  
Shriram Nallamshetty ◽  
Jeramie D. Wartrous ◽  
Eowyn Acres ◽  
Tao Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Room Temperature ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Resource Limited ◽  
Metabolomics Study ◽  
Simultaneous Measure ◽  
Analytical Approaches ◽  
And Storage

Advances in high resolution, nontargeted mass spectrometry allow for the simultaneous measure of thousands of metabolites in a single biosample. Application of these analytical approaches to population-scale human studies has been limited by the need for resource-intensive blood sample collection, preparation, and storage. Dried blood spotting, a technique developed decades ago for newborn screening, may offer a simple approach to overcome barriers in human blood acquisition and storage. In this study, we find that over 4,000 spectral features across diverse chemical classes may be efficiently and reproducibly extracted and relatively quantified from human dried blood spots using nontargeted mass spectrometry-based metabolomics. Moreover, over 80% of metabolites were found to be chemically stable in dried blood spots stored at room temperature for up to a week. In direct relation to plasma samples, dried blood spots exhibited comparable representation of the human circulating metabolome, capturing both known and previously uncharacterized metabolites. Dried blood spot approaches provide an opportunity for rapid and facile human biosampling and storage, and will enable widespread metabolomics study of populations, particularly in resource-limited areas.

Adaptation of Elecsys® anti-severe acute respiratory syndrome coronavirus-2 immunoassay to dried blood spots: proof of concept

Bioanalysis ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 165-171
Author(s):  
Alexandre Marchand ◽  
Ingrid Roulland ◽  
Florian Semence ◽  
Magnus Ericsson
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Dried Blood Spots ◽  
Proof Of Concept ◽  
Past Infection ◽  
Blood Spots ◽  
And Storage

Aim: Several automated immunoassays have been validated on serum/plasma to evaluate the presence of significant levels of anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) antibodies, signs of a present or past infection, but the use of dried blood spots (DBS) would facilitate sampling, shipping and storage. Objective: The aim of this project was to give proof of concept of the possibility to use of the automatized Elecsys® anti-SARS-CoV-2 immunoassay with a volumetric DBS device. Results: Linearity and correlation were satisfactory between volumetric DBS and plasma. A cut-off value was suggested and should be validated with more samples. Conclusion: this study strongly support the possibility to work with volumetric DBS instead of serum/plasma to test for anti-SARS-CoV-2 antibodies.

scholarly journals Separation of Plasma from Whole Blood by Use of the cobas Plasma Separation Card: a Compelling Alternative to Dried Blood Spots for Quantification of HIV-1 Viral Load

2019 ◽  
Vol 57 (4) ◽  
Author(s):  
Sergio Carmona ◽  
Britta Seiverth ◽  
Dieketseng Magubane ◽  
Lucia Hans ◽  
Matthias Hoppler
Keyword(s):  
Viral Load ◽  
Whole Blood ◽  
Health Workers ◽  
Dried Blood Spots ◽  
Plasma Separation ◽  
Blood Spots ◽  
Specificity And Sensitivity ◽  
Finger Prick ◽  
And Storage ◽  
Hiv 1

ABSTRACTPlasma HIV viral load testing is the preferred means of monitoring antiretroviral treatment response. Dried blood spots (DBSs) hold considerable logistical advantages over EDTA samples, but they more frequently misclassify virological failure and have higher limits of detection (LoD). Plasma separation cards (PSCs) may overcome these limitations. Health workers collected EDTA whole blood by venipuncture and 140 μl of finger-prick blood by capillary tube from 53 HIV-infected adults. Capillary blood was immediately transferred to PSCs. Additionally, 432 EDTA samples from HIV-infected adults were spotted onto PSCs and analyzed together with the finger-prick samples. Specificity and sensitivity of PSC with paired EDTA-PSC samples tested on a cobas 6800/8800 system with the cobas HIV-1 test (cobas HIV) was determined. LoD (3rd HIV-1 WHO International Standard) and stability at a range of temperatures and storage durations was determined using cobas HIV and cobas AmpliPrep/cobas TaqMan HIV-1 test v2.0 (CAP/CTM). Of 132 specimens with quantitative values for paired EDTA-PSC samples, the mean log10difference between samples was 0.05 copies/ml (95% confidence interval [CI], −0.01 to 0.11). The LoD for cobas HIV was 790.2 copies/ml and for CAP/CTM was 737.9 copies/ml. At 1,000 copies/ml, PSC sensitivity was 97.0% (128/132) and specificity was 97.2% (343/353). Results correlated well with those from EDTA samples (DemingR2= 0.90). PSC results were unaffected by temperature and storage conditions. PSC samples correlate well with plasma viral load and have adequate sensitivity and specificity. The improved performance may be as a result of a reduction in contribution from cell-associated viral nucleic acids. The card provides an alternative sample collection technology to DBSs.

Therapeutic drug monitoring (TDM) of the recent antipsychotic ziprasidone in dried blood spots (DBS) and plasma

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
L Mercolini ◽  
G Fulgenzi ◽  
M Melis ◽  
G Boncompagni ◽  
LJ Albers ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Blood Spots
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