Adjuvant and immunostimulatory effects of β-glucan administration in combination with lipopolysaccharide enhances survival and some immune parameters in carp challenged with Aeromonas hydrophila

The fermentable fiber, Vitacel R200 is industrial compound cellulose exerting many beneficial health effects at the hosts. In this study, rainbow trout (Oncorhynchus mykiss) fingerling (120 ± 4.5 grams) were fed dietary 1% (10 g/kg feed) Vitacel R200 for 7 weeks, then challenged with Aeromonas hydrophila. Two weeks after the challenge, hemato-biochemical and immune parameters in the challenged fish were studied. The lysozyme and complement activity, as well as, white blood cells (WBCs), hemoglobin (Hb%), percentage of neutrophil and eosinophil in the fish fed Vitacel were significantly increased compared with the control group. Furthermore, the serum total protein, albumin, Na+, K+, P, and Ca++ concentrations in the fish fed diet containing 1% Vitacel were significantly increased, while alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST), as well as, cortisol, cholesterol, and glucose concentration were significantly decreased in comparison to the control group. These results concluded that dietary Vitacel could improve the hemato-biochemical and immune parameters, as well as, the stress response in O. mykiss infected with A. hydrophila.

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Effect of orally administered azadirachtin on non-specific immune parameters of goldfish Carassius auratus (Linn. 1758) and resistance against Aeromonas hydrophila

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2012.11.038 ◽

2013 ◽

Vol 34 (2) ◽

pp. 564-573 ◽

Cited By ~ 55

Author(s):

Saurav Kumar ◽

R.P. Raman ◽

P.K. Pandey ◽

Snatashree Mohanty ◽

Abhay Kumar ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Carassius Auratus ◽

Goldfish Carassius Auratus ◽

Immune Parameters

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Effect of Curcuma longa on Non-specific immunological profile of Indian Snakehead Channa punctatus infected with Aeromonas hydrophila

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.6(2).p59-63 ◽

2016 ◽

Vol 6 (2) ◽

pp. 59-63

Author(s):

Seema Jain ◽

Manu Varma

Keyword(s):

Body Weight ◽

Disease Resistance ◽

Aeromonas Hydrophila ◽

Curcuma Longa ◽

Opportunistic Pathogen ◽

Channa Punctatus ◽

Phagocytic Index ◽

Maximum Enhancement ◽

Superoxide Anion Production ◽

Immune Parameters

Curcumin, an Orange Yellow phytochemical, hydrophobic and polyphenolic compound of Turmeric (Curcuma longa.Linn.) has been known to be a potent immunomodulatory agent in many animal species. The aim of our study was to evaluate the immunomodulatory properties and disease resistance against opportunistic pathogen Aeromonas hydrophila in Channa punctatus. Fishes were challenged intraperitoneally with a dose of A. hydrophila(1 ×108 cells/ ml.). Immunocompromised fishes were given three different doses of Curcumin viz. 0.5 mg, 1.0 mg, 1.5 mg / 100gm of body weight respectively. Treated Fishes were sacrificed on 3, 5,7 and 10 day after the dose was given and various Non specific immune parameters were assessed viz. Percentage Phagocytosis, Phagocytic Index, Lysozyme assay, Bactericidal activity and Superoxide anion Production. All the Nonspecific parameters were enhanced significantly. Maximum enhancement was seen on the 5th day with a dose of 1mg/ 100gm body weight and the same dose also showed maximum disease resistance as compared to other two doses.

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