Chemical composition and efficacy of dichloromethane extract of Croton sphaerogynus Baill. (Euphorbiaceae) against the cattle tick Rhipicephalus microplus (Acari: Ixodidae)

Slow pyrolysis is a process for the thermochemical conversion of biomasses into bio-oils that may contain a rich chemical composition with biotechnological potential. Bio-oil produced from crambe fruits was investigated as to their acaricidal effect. Slow pyrolysis of crambe fruits was performed in a batch reactor at 400°C and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The bio-oil collected was used in bioassays with larvae and engorged females of the cattle tick Rhipicephalus microplus. Biological assays were performed using the larval packet test (LPT) and adult immersion test. The GC-MS of crambe fruit bio-oil revealed mainly hydrocarbons such as alkanes and alkenes, phenols, and aldehydes. The bio-oil in the LPT exhibited an LC90 of 14.4%. In addition, crambe bio-oil caused female mortality of 91.1% at a concentration of 15% and a high egg-laying inhibition. After ovary dissection of treated females, a significant reduction in gonadosomatic index was observed, indicating that bio-oil interfered in tick oogenesis. Considering these results, it may be concluded that slow pyrolysis of crambe fruit affords a sustainable and eco-friendly product for the control of cattle tick R. microplus.

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Efficacy of two commercial synthetic pyrethroids (cypermethrin and deltamethrin) on Amblyomma variegatum and Rhipicephalus microplus strains of the south-western region of Burkina Faso

Tropical Animal Health and Production ◽

10.1007/s11250-021-02849-2 ◽

2021 ◽

Vol 53 (3) ◽

Author(s):

Achille S. Ouedraogo ◽

Olivier M. Zannou ◽

Abel S. Biguezoton ◽

Kouassi Yao Patrick ◽

Adrien M. G. Belem ◽

...

Keyword(s):

Burkina Faso ◽

Pyrethroid Resistance ◽

Tick Control ◽

Rhipicephalus Microplus ◽

Amblyomma Variegatum ◽

Synthetic Pyrethroid ◽

Cattle Tick ◽

Control Problems ◽

Synthetic Pyrethroids ◽

Western Region

AbstractSince 2011, period of the livestock invasion by the cattle tick Rhipicephalus microplus in Burkina Faso (BF), tick-control problems were exacerbated. Based on farmer’s reports, most commonly used commercial acaricides were found to be ineffective in Western South part of the country. To investigate the occurrence and extent of such acaricidal ineffectiveness, we performed the standardized larval packet test (LPT) with commercial deltamethrin (vectocid) and cypermethrin (cypertop), on two cattle tick species, the native Amblyomma variegatum and the invasive R. microplus. The resistance ratios (RR) were computed with susceptible Hounde strain of Rhipicephalus geigyi as reference. The R. microplus population showed resistance to the two acaricides tested with the highest lethal concentration (LC) values, and different resistance ratios higher than 4 (deltamethrin: RR50 = 28.18 and RR90 = 32.41; cypermethrin: RR50 = 8.79 and RR90 = 23.15). In the contrary, A. variegatum population was found to be highly susceptible to acaricides tested with low lethal concentrations and resistance ratio values (deltamethrin: RR50 = 0.5 and RR90 = 0.48; cypermethrin: RR50 = 0.68 and RR90 = 0.79). These data demonstrate high synthetic pyrethroid resistance in R. microplus strain, leading to conclude that the acaricide ineffectiveness in tick populations control remains a concern in BF.

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Chemical Composition and Acaricidal Activity of Nemuaron vieillardii Essential Oil against the Cattle Tick Rhipicephalus (Boophilus) microplus

Natural Product Communications ◽

10.1177/1934578x1601101235 ◽

2016 ◽

Vol 11 (12) ◽

pp. 1934578X1601101

Author(s):

Nicolas Lebouvier ◽

Thomas Hue ◽

Joseph Brophy ◽

Edouard Hnawia ◽

Mohammed Nour

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Tick Control ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

Gas Chromatography Mass Spectrometry ◽

Cattle Tick ◽

Caryophyllene Oxide ◽

Human Carcinogen

Essential oil from leaves of Nemuaron vieillardii (Baill.) Baill., a shrub used in the kanak pharmacopeia, was analyzed by gas chromatography and combined gas chromatography-mass spectrometry. The main compounds identified were safrole (49.7%), linalool (8.0%), δ-cadinene (5.1%), caryophyllene oxide (4%) and α-copaene (2.4%). This chemical composition is consistent with leaf oils of the Atherospermataceae family and demonstrates the proximity of the two genera Nemuaron and Atherosperma as regards to their essential oil compositions. The modified Larval Packet Test (LPT) was used to assess acaricidal effect of N. vieillardii essential oil on larvae of the cattle tick Rhipicephalus (Boophilus) microplus and LC50 was then calculated to 14.67%. High content of safrole in this oil which is suspected of being a human carcinogen, poses the problem of the use of this oil in a perspective of development of alternative tick control strategy and in the traditional medicinal consumption of Nemuaron vieillardii.

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Chemical composition and acaricidal activity of Lantana camara L. and Lantana montevidensis Briq. essential oils on the tick Rhipicephalus microplus

Journal of Essential Oil Research ◽

10.1080/10412905.2020.1752320 ◽

2020 ◽

Vol 32 (4) ◽

pp. 316-322

Author(s):

Erlânio Oliveira De Sousa ◽

Aldilene Da Silva Lima ◽

Suzana Gomes Lopes ◽

Lívio Martins Costa-Junior ◽

José Galberto Martins Da Costa

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Lantana Camara

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Acaricidal activity of leaves of Morus nigra against the cattle tick Rhipicephalus microplus

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-8994 ◽

2017 ◽

Vol 69 (3) ◽

pp. 523-528 ◽

Cited By ~ 1

Author(s):

A.C.S. Dantas ◽

D.P. Freire ◽

G.R. Souza ◽

J.R.G.S. Almeida ◽

L.A. Rolim ◽

...

Keyword(s):

Phenolic Compounds ◽

Ethanolic Extract ◽

Adult Tick ◽

Immersion Test ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Cattle Tick ◽

Chloroform Fraction ◽

Morus Nigra ◽

Cattle Ticks

ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of eggs eclosion average and 65.4% of effectiveness. HPLC analysis revealed the presence of phenolic compounds, which may be related to biological activity shown by the extracts, which can be used as an alternative control against R. microplus adult tick.

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Mutation in the Rm AOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1309072110 ◽

2013 ◽

Vol 110 (42) ◽

pp. 16772-16777 ◽

Cited By ~ 27

Author(s):

S. W. Corley ◽

N. N. Jonsson ◽

E. K. Piper ◽

C. Cutulle ◽

M. J. Stear ◽

...

Keyword(s):

Rhipicephalus Microplus ◽

Cattle Tick

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Draft Genome Sequence of Enterococcus casseliflavus PAVET15 Obtained from the Oviduct Infection of the Cattle Tick (Rhipicephalus microplus) in Jiutepec, Morelos, Mexico

Genome Announcements ◽

10.1128/genomea.00196-17 ◽

2017 ◽

Vol 5 (16) ◽

Author(s):

R. Cossío-Bayúgar ◽

E. Miranda-Miranda ◽

C. A. Arreguín-Pérez ◽

L. Lozano ◽

D. Peréz de la Rosa ◽

...

Keyword(s):

Lactic Acid ◽

Genome Sequence ◽

Draft Genome ◽

Rhipicephalus Microplus ◽

Draft Genome Sequence ◽

Cattle Tick ◽

Gram Positive ◽

Content Type ◽

Enterococcus Casseliflavus ◽

Acid Producing Bacteria

ABSTRACT Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 contigs, an N 50 of 179,476 bp, and 41.93% G+C content.

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In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

The Scientific World JOURNAL ◽

10.1155/2014/858973 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

S. Parveen ◽

R. Godara ◽

R. Katoch ◽

A. Yadav ◽

P. K. Verma ◽

...

Keyword(s):

Immersion Test ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Cattle Tick ◽

Egg Mass ◽

Ageratum Conyzoides ◽

Artemisia Absinthium ◽

Aerial Parts ◽

Ethanolic Extracts

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.

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