Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type 2

2006 ◽  
Vol 139 (3) ◽  
pp. 607-614 ◽  
Author(s):  
Chie Aoki ◽  
Kazuya I.P.J. Hidari ◽  
Saki Itonori ◽  
Akihiro Yamada ◽  
Naonori Takahashi ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Identification And Characterization ◽  
Dengue Virus Type 2

Antiviral activity of Faramea bahiensis leaves on dengue virus type-2 and characterization of a new antiviral flavanone glycoside

2017 ◽  
Vol 19 ◽  
pp. 220-225 ◽  
Author(s):  
Adriana C. Nascimento ◽  
Ligia M.M. Valente ◽  
Mário Gomes ◽  
Rodolfo S. Barboza ◽  
Thiago Wolff ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Virus Type ◽  
Flavanone Glycoside ◽  
Dengue Virus Type 2

Establishment and characterization of dengue virus type 2 nonstructural protein 1 specific T cell lines

2010 ◽  
Vol 33 (6) ◽  
pp. e75-e80 ◽  
Author(s):  
Yang Xiao-meng ◽  
Jiang Li-fang ◽  
Tang Yun-xia ◽  
Yin Yue ◽  
Liu Wen-quan ◽  
...  
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
Cell Lines ◽  
Virus Type ◽  
Nonstructural Protein ◽  
Nonstructural Protein 1 ◽  
T Cell Lines ◽  
Dengue Virus Type 2

scholarly journals Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains

Virology ◽  
2008 ◽  
Vol 377 (2) ◽  
pp. 296-307 ◽  
Author(s):  
Nikos Vasilakis ◽  
Eric B. Fokam ◽  
Christopher T. Hanson ◽  
Ethan Weinberg ◽  
Amadou A. Sall ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Phenotypic Characterization ◽  
Dengue Virus Type 2

scholarly journals Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation

2006 ◽  
Vol 87 (8) ◽  
pp. 2263-2268 ◽  
Author(s):  
Dennis J. Pierro ◽  
Ma Isabel Salazar ◽  
Barry J. Beaty ◽  
Ken E. Olson
Keyword(s):  
Cell Culture ◽  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Infectious Clone ◽  
Cell Types ◽  
Dengue Virus Type 2

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.

scholarly journals Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Kleber Juvenal Silva Farias ◽  
Paula Renata Lima Machado ◽  
Benedito Antônio Lopes da Fonseca
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Plaque Assay ◽  
Antiviral Drug ◽  
Vero Cells ◽  
Significant Difference ◽  
Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

scholarly journals Clinical and Epidemiological Characterization of the First Recognized Outbreak of Dengue Virus-Type 2 in Mozambique, 2014

2016 ◽  
Vol 94 (2) ◽  
pp. 413-416 ◽  
Author(s):  
Marilia Massangaie ◽  
Janusz Paweska ◽  
Celia Chirindza ◽  
Jacqueline Weyer ◽  
Eduardo Samo Gudo ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Epidemiological Characterization ◽  
Dengue Virus Type 2

scholarly journals Characterization of Dengue Virus Type 2: New Insights on the 2010 Brazilian Epidemic

PLoS ONE ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. e11811 ◽  
Author(s):  
Camila Malta Romano ◽  
Andréia Manso de Matos ◽  
Evaldo Stanislau A. Araújo ◽  
Lucy Santos Villas-Boas ◽  
Wanessa Cardoso da Silva ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Dengue Virus Type 2

scholarly journals Domain-III FG loop of the dengue virus type 2 envelope protein is important for infection of mammalian cells and Aedes aegypti mosquitoes

Virology ◽  
2010 ◽  
Vol 406 (2) ◽  
pp. 328-335 ◽  
Author(s):  
Steven M. Erb ◽  
Siritorn Butrapet ◽  
Kelley J. Moss ◽  
Betty E. Luy ◽  
Thomas Childers ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Envelope Protein ◽  
Mammalian Cells ◽  
Domain Iii ◽  
Dengue Virus Type 2
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