F gene recombination between genotype II and VII Newcastle disease virus

2008 ◽  
Vol 131 (2) ◽  
pp. 299-303 ◽  
Author(s):  
Zhuoming Qin ◽  
Lei Sun ◽  
Baochen Ma ◽  
Zhizhong Cui ◽  
Yiping Zhu ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Gene Recombination ◽  
F Gene ◽  
Genotype Ii

scholarly journals Complete Genome Sequences of Two Newcastle Disease Virus Strains Isolated from a Wild Duck and a Pigeon in Russia

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Marsel R. Kabilov ◽  
Tatyana Y. Alikina ◽  
Kseniya S. Yurchenko ◽  
Alexandra V. Glushchenko ◽  
Konstantin V. Gunbin ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Complete Genome ◽  
Comparative Sequence Analysis ◽  
Genome Sequences ◽  
Wild Duck ◽  
F Gene ◽  
Virus Strains

Here, we report the complete genome sequences of two Newcastle disease virus (NDV) isolates, Adygea/duck/12/2008, from a wild duck in Russia, and Altai/pigeon/777/2010, from a pigeon in Russia. Based on comparative sequence analysis of the F gene, these strains were classified as NDV class II, genotypes VIId and VIb/2, respectively.

Isolation and Molecular Characterization of Newcastle Disease Virus in Layers

2021 ◽  
Author(s):  
Smita Bordoloi ◽  
Anju Nayak ◽  
A.P. Singh ◽  
R.V. Singh ◽  
Kajal Jadav ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virulent Strain ◽  
Indian Subcontinent ◽  
Economic Losses ◽  
Tissue Samples ◽  
F Gene ◽  
Virulent Newcastle Disease Virus

Background: Newcastle disease (ND) in spite of the availability of vaccines remains a constant threat to poultry producers worldwide. It is prevalent in Indian subcontinent and leads to economic losses. The present study was aimed with isolate and identify virulent Newcastle disease virus (NDV) in layer poultry from field outbreaks.Methods: Total 47 samples consisting of nasal (05), oropharyngeal (13) and cloacal swabs (11) and tissue samples consisting of trachea (07), lungs (06), larynx (05) were collected from layer birds. For isolation of NDV swab and tissue samples were inoculated in 9-11 days old embryonated eggs via allantoic cavity route. After preparing the viral inoculum, 47 suspected samples (29 swab and 18 tissue samples) were inoculated in 141 embryonated eggs to isolate the virus.Result: Out of 47 samples 10 (21.27%) samples were positive for HA activity. All the 10 isolates showing HA activity subjected to Reverse-Transcriptase PCR of F gene and 6 were found positive in RT-PCR for F1 gene. The PCR amplified product showed amplicon at 356 bp and 254 bp positive for F1 and F2 gene, respectively. On basis of F gene, 06 (50%) isolates were considered as virulent Newcastle Disease Virus. One isolate sequence was submitted at NCBI with accession MT890653 On phylogenetic analysis MT890653 designated as Class II/ genotype II/ virulent strain and had the motif 112R-R-R-K-R-F117 at the cleavage site of the fusion protein.

scholarly journals Persistence of Newcastle Disease Virus Genotype II and Shedded Genotype VII in Poultry Farm Environment

2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Fady Samir ◽  
Rania F. El Naggar ◽  
Mohamed M. Hamoud ◽  
Manal M. Zaki ◽  
Abdulrhman M. Gamal ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Poultry Farm ◽  
Virus Genotype ◽  
Genotype Ii ◽  
Genotype Vii

scholarly journals Pathotyping of Newcastle disease virus: A novel single BsaHI digestion method of detection and differentiation of avirulent strains (lentogenic and mesogenic vaccine strains) from virulent virus

2021 ◽  
Author(s):  
Perumal Arumugam Desingu ◽  
Shambhu Dayal Singh ◽  
Kuldeep Dhama ◽  
Obli Rajendran Vinodhkumar ◽  
K Nagarajan ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Rt Pcr ◽  
Specific Primer ◽  
Digestion Method ◽  
F Gene ◽  
Virulent Strains ◽  
Virulent Virus ◽  
Short Space

We provide a novel single restriction enzyme (RE) (BsaHI) digestion approach for detecting distinct pathotypes of the Newcastle disease virus (NDV). After scanning 4000 F gene nucleotide sequences in the NCBI database, a single RE (BsaHI) digesting site was discovered in the cleavage site. APMV-I "F gene" Class II specific primer-based reverse transcriptase PCR (RT-PCR) was utilized to amplify a 535 bp fragment, which was then digested with a single RE (BsaHI) for pathotyping avian NDV field isolates and pigeon paramyxovirus-1 isolates. The avirulent (lentogenic and mesogenic strains) produce 189 and 346 bp fragments, respectively, but the result in velogenic strains remains undigested with 535 bp fragments. In addition, 45 field NDV isolates and 8 vaccine strains were used to confirm the approach. The sequence-based analysis also agrees with the data obtained utilizing the single RE (BsaHI) digestion approach. The proposed technique had the potential to distinguish between avirulent and virulent strains in a short space of time, making it valuable in NDV surveillance and monitoring research.

scholarly journals Characterization of Newcastle Disease Virus in Broiler Flocks with Respiratory symptoms in some Provinces of Iran

2021 ◽  
Author(s):  
Foroogh Makki ◽  
Zahra Boroomand ◽  
Mansour Mayahi ◽  
Masoud Reza Seyfi Abad Shapouri
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Respiratory Symptoms ◽  
Allantoic Fluid ◽  
Economic Losses ◽  
Protein Cleavage ◽  
Khuzestan Province ◽  
East Azerbaijan ◽  
Genotype Ii

Abstract Background: Newcastle disease, is one of the most important diseases of the poultry industry, has many economic losses. The aim of this study was to isolate and determine the molecular identity of Newcastle disease virus in 40 broiler flocks with respiratory symptoms in four provinces of Iran.Methods and Results: Samples of farms with respiratory symptoms were collected from different regions of Isfahan, East Azerbaijan, Golestan, and Khuzestan provinces and inoculated into 9-day-old embryonated chicken eggs. The Reverse-transcription polymerase chain reaction (RT-PCR) was performed to detect the Newcastle disease virus on allantoic fluid. Of the 40 flocks, the virus was isolated and identified in 16 flocks. The PCR products of 16 isolates were sequenced and a phylogenetic tree was drawn. Accordingly, six isolates were in genotype II and ten isolates were in subgenotype VIId of class II. Conclusion: Both genotypes were present in all four provinces. The isolates of Khuzestan province showed the greatest diversity compared to the other three provinces. The similarity of isolates belonging to genotype II in this study was observed with Pakistan, China, and Nigeria and other isolates were similar to previous isolates in Iran. Also, the highest amino acid sequence in the F-protein cleavage site was 112RRQKR/F117 for VIId genotype isolates and 112GRQGR/L117 for II genotype isolates.

scholarly journals Molecular Characterization and Pathology of Virulent Newcastle Disease Virus Isolated from Broiler Chickens in District Lahore, Pakistan

2021 ◽  
Author(s):  
M. Ali ◽  
M.U.R. Khan ◽  
A. Aslam ◽  
H.U. Rehman ◽  
A. Anjum ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Molecular Detection ◽  
Histopathological Examination ◽  
Genotype I ◽  
Pathogenic Potential ◽  
F Gene ◽  
Virulent Newcastle Disease Virus

Background: This study elucidated the molecular detection and pathological alterations in broiler chickens naturally infected with field circulating NDV strains along with their phylogenomic dynamics. Methods: Morbid tissue samples of diseased/dead chickens were collected from 100 poultry flocks presented to poultry disease diagnostic laboratories from September 2018 to August 2019. Samples were subjected to molecular detection of NDV along with phylogenetic analysis and subsequent gross and histopathological examination. Result: Based on RT-PCR results, the positivity of NDV was 04/100 (4%). Genetic analysis of the NDV Fusion (F) gene revealed 98.92% and 98.74% similarity with Iranian and Pakistani isolates, respectively. The evolutionary tree showed that present study isolates were placed in a clade belongs to genotype Vll sub-genotype i and l. Necropsy examinations revealed the petechial haemorrhages associated with multifocal necrosis in gastrointestinal and respiratory organs. Besides these pathological findings, amino acid sequence of F gene revealed that study isolates are having pathogenic potential similar to the velogenic strains of NDV. Based on all essential analyses, the present study concluded that the evolution and distribution of the Newcastle disease virus of various genotypes VIIi and VIIl in Pakistan are having significant pathogenic potential. Therefore, it emphasizes developing ND vaccine from indigenous strains for better protection of commercial poultry in Pakistan.

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