Genetic Characterization and Pathogenesis of Three Novel Reassortant H5N2 Viruses in South Korea, 2018

The outbreaks of H5N2 avian influenza viruses have occasionally caused the death of thousands of birds in poultry farms. Surveillance during the 2018 winter season in South Korea revealed three H5N2 isolates in feces samples collected from wild birds (KNU18-28: A/Wild duck/South Korea/KNU18-28/2018, KNU18-86: A/Bean Goose/South Korea/KNU18-86/2018, and KNU18-93: A/Wild duck/South Korea/KNU18-93/2018). Phylogenetic tree analysis revealed that these viruses arose from reassortment events among various virus subtypes circulating in South Korea and other countries in the East Asia–Australasian Flyway. The NS gene of the KNU18-28 and KNU18-86 isolates was closely related to that of China’s H10N3 strain, whereas the KNU18-93 strain originated from the H12N2 strain in Japan, showing two different reassortment events and different from a low pathogenic H5N3 (KNU18-91) virus which was isolated at the same day and same place with KNU18-86 and KNU18-93. These H5N2 isolates were characterized as low pathogenic avian influenza viruses. However, many amino acid changes in eight gene segments were identified to enhance polymerase activity and increase adaptation and virulence in mice and mammals. Experiments reveal that viral replication in MDCK cells was quite high after 12 hpi, showing the ability to replicate in mouse lungs. The hematoxylin and eosin-stained (H&E) lung sections indicated different degrees of pathogenicity of the three H5N2 isolates in mice compared with that of the control H1N1 strain. The continuing circulation of these H5N2 viruses may represent a potential threat to mammals and humans. Our findings highlight the need for intensive surveillance of avian influenza virus circulation in South Korea to prevent the risks posed by these reassortment viruses to animal and public health.

Dvokrilnost istine u Divljoj patki: Platon, Ibsen i Krleža

Questioning the commonly held assumption in critical reception that Ibsenʼs symbol of the wild duck was influenced by Darwinʼs theory, I want to argue that the wild duck flew into Ibsenʼs play all the way from Platoʼs aporetic dialogue The Theaetetus. Following Lacanʼs reading of Plato, I want to examine the connection between the Socratic position towards knowledge – especially the rupture between knowledge and truth – and the treatment of dramatic dialogue in Ibsenʼs The Wild Duck and Krležaʼs The Glembays.

Emergence of a Novel Reassortant H5N3 Avian Influenza Virus in Korean Mallard Ducks in 2018

<b><i>Introduction:</i></b> The avian influenza (AI) virus causes a highly contagious disease which is common in wild and domestic birds and sporadic in humans. Mutations and genetic reassortments among the 8 negative-sense RNA segments of the viral genome alter its pathogenic potential, demanding well-targeted, active surveillance for infection control. <b><i>Methods:</i></b> Wild duck fecal samples were collected during the 2018 bird health annual surveillance in South Korea for tracking variations of the AI virus. One low-pathogenic avian influenza H5N3 reassortment virus (A/mallard duck/South Korea/KNU18-91/2018 [H5N3]) was isolated and genomically characterized by phylogenetic and molecular analyses in this study. <b><i>Results:</i></b> It was devoid of polybasic amino acids at the hemagglutinin (HA) cleavage site and exhibited a stalk region without deletion in the neuraminidase (<i>NA</i>) gene and NA inhibitor resistance-linked E/D627K/N and D701N marker mutations in the <i>PB2</i> gene, suggesting its low-pathogenic AI. It showed a potential of a reassortment where only <i>HA</i> originated from the H5N3 poultry virus of China and other genes were derived from Mongolia. In phylogenetic analysis, HA was different from that of the isolate of H5N3 in Korea, 2015. In addition, this novel virus showed adaptation in Madin-Darby canine kidney cells, with 8.05 ± 0.14 log10 50% tissue culture infectious dose (TCID50) /mL at 36 h postinfection. However, it could not replicate in mice well, showing positive growth at 3 days postinfection (dpi) (2.1 ± 0.13 log10 TCID50/mL) but not at 6 dpi. <b><i>Conclusions:</i></b> The HA antigenic relationship of A/mallard duck/South Korea/KNU18-91/2018 (H5N3) showed differences toward one of the old low-pathogenic H5N3 viruses in Korea. These results indicated that a novel reassortment low-pathogenic avian influenza H5N3 subtype virus emerged in South Korea in 2018 via novel multiple reassortments with Eurasian viruses, rather than one of old Korean H5N3 strains.

SOME ASPECTS OF VETERINARY AND SANITARY EXAMINATION OF WILD DUCK MEAT IN SARCOCYSTOSIS

Now hunting for wild animals and birds is widespread in the Russian Federation. However, the veterinary and sanitary examination was not fully elaborated for a number of parasitic diseases, in particular for sarcocystosis of ducks. It is one of the most widespread avian diseases and it is increasingly being encountered by biologists, hunters and veterinarians. The article presents parameters for evaluating the meat of clinically healthy ducks and those infected with sarcocysts. The results obtained allow us to conclude about the prevalence and the need to improve the veterinary and sanitary assessment of meat and the system of preventive measures for sarcocystic invasion. Key words: sarcocystosis, hunting, ducks.

The Evolutionary Dynamics of Influenza A Viruses Circulating in Mallards in Duck Hunting Preserves in Maryland, USA

Duck hunting preserves (DHP) have resident populations of farm-raised mallard ducks, which create potential foci for the evolution of novel influenza A viruses (IAVs). Through an eleven-year (2003–2013) IAV surveillance project in seven DHPs in Maryland, USA, we frequently identified IAVs in the resident, free-flying mallard ducks (5.8% of cloacal samples were IAV-positive). The IAV population had high genetic diversity, including 12 HA subtypes and 9 NA subtypes. By sequencing the complete genomes of 290 viruses, we determined that genetically diverse IAVs were introduced annually into DHP ducks, predominantly from wild birds in the Anatidae family that inhabit the Atlantic and Mississippi flyways. The relatively low viral gene flow observed out of DHPs suggests that raised mallards do not sustain long-term viral persistence nor do they serve as important sources of new viruses in wild birds. Overall, our findings indicate that DHPs offer reliable samples of the diversity of IAV subtypes, and could serve as regional sentinel sites that mimic the viral diversity found in local wild duck populations, which would provide a cost-efficient strategy for long-term IAV monitoring. Such monitoring could allow for early identification and characterization of viruses that threaten bird species of high economic and environmental interest.

Values of Life in The Wild Duck by Hendrik Ibsen

This study aims to analyze and discuss the values in Hendrik Ibsen’s The Wild Duck. The method used in this study is descriptive. The data are a drama entitled The Wild Duck (1959). A library research method is applied in collecting the data. Furthermore, the data is analyzed qualitatively. Based on the result of the analysis, it was revealed that there are five kinds of Values found in The Wild Duck which are Moral Value, Social Value, Personal Value, Spiritual Value and Family Value. The most presented type is a moral value, it is about honesty. Social value is about interaction with around, friendship, respect to each other, justice. A personal value is a personality. Spiritual is non-material, it means the values of truth, inner peace, righteousness, and non-violence and the last is family values are beliefs, function as a family, ideals.