Chikungunya virus titration, detection and diagnosis using N-Acetylglucosamine (GlcNAc) specific lectin based virus capture assay

2021 ◽  
pp. 198493
Author(s):  
Shweta Choudhary ◽  
Neetu Neetu ◽  
Vedita Anand Singh ◽  
Pravindra Kumar ◽  
Madhulika Chaudhary ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
Capture Assay ◽  
Virus Titration ◽  
Detection And Diagnosis

scholarly journals The inability of a dengue NS1 ELISA to detect Zika infections

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Monique da Rocha Queiroz Lima ◽  
Thais Chouin-Carneiro ◽  
Elzinandes Azeredo ◽  
Luciana Santos Barbosa ◽  
Thiara Manuele Alves Souza ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Dengue Virus ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
Signs And Symptoms ◽  
High Accuracy ◽  
Cross Reactivity ◽  
High Rate ◽  
Difficult Diagnosis ◽  
Capture Assay

AbstractThe presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here, we evaluated a Dengue NS1 capture assay for early and differential diagnosis of dengue during the Zika epidemic occurred in Brazil in 2016. Samples (n = 227) from 218 patients included sera, plasma and urine from previously confirmed acute cases of Zika, dengue and Zika/dengue co-infections. Nine of those patients presented two specimens. The Dengue NS1 test was very specific for dengue diagnosis (99.32%), even in the co-circulation with ZIKV, and exhibited a high accuracy in not detecting acute Zika infections (92.43%). Our findings showed that the dengue NS1 capture test analyzed here was not able to recognize the ZIKV NS1 and its potential for cross-reaction.

PAICA: A Method for Characterizing Platelet-Associated Antibodies - Its Application to the Study of Idiopathic Thrombocytopenic Purpura and to the Detection of Platelet-bound c7E3

1996 ◽  
Vol 76 (06) ◽  
pp. 1020-1029 ◽  
Author(s):  
Laurent Macchi ◽  
Gisèle Clofent-Sanchez ◽  
Gérald Marit ◽  
Claude Bihour ◽  
Catherine Durrieu-Jais ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Idiopathic Thrombocytopenic Purpura ◽  
Antithrombotic Therapy ◽  
Membrane Glycoproteins ◽  
Serum Antibodies ◽  
Platelet Destruction ◽  
Thrombocytopenic Purpura ◽  
Capture Assay ◽  
Specific Membrane

SummaryIn idiopathic thrombocytopenic purpura (ITP), autoantibodies reacting with antigens on the platelet membrane bring about accelerated platelet destruction. We now report PAICA (“Platelet-Associated IgG Characterization Assay”), a method for detecting autoantibodies bound to specific membrane glycoproteins in total platelet lysates. This monoclonal antibody (MAb) capture assay takes into account the fact that antibodies on circulating platelets may be translocated to internal pools as well as being on the surface. A total of twenty ITP patients were examined by PAICA, and the results compared with those obtained by measuring (i) serum antibodies bound to paraformaldehyde-fixed control platelets by ELISA, (ii) IgG bound to the surface of the patient’s own platelets by flow cytometry (PSIgG), (iii) total platelet-associated IgG (PAIgG) by ELISA and (iv) serum antibodies reacting with control platelets by MAIPA (“Monoclonal Antibody-specific Immobilization of Platelet Antigens”). Of twelve patients with elevated PAIgG, nine had increased PSIgG yet eleven reacted positively in PAICA. Of these, eight possessed antibodies directed against GP Ilb-IIIa, two against GP Ib-IX and one patient possessed antibodies directed against GP Ilb-IIIa and GP Ia-IIa respectively. Only seven of the patients possessed serum antibodies detectable by MAIPA. PAICA was also able to detect platelet-associated c7E3 (the chimeric form of Fab fragments of the MAb 7E3) following its infusion during antithrombotic therapy, when it proved more sensitive over a seven-day period than a MAIPA assay adapted for assessing surface-bound antibody. We propose that PAICA provides added sensitivity to the detection of platelet-associated antibodies in immune thrombocytopenias or following therapy with humanized MAbs.

Detection of Leukemia Using Image Processing

2018 ◽  
Vol 8 (4) ◽  
pp. 61
Author(s):  
A. Sivasangari ◽  
G. Sasikumar
Keyword(s):  
Image Processing ◽  
Statistical Analysis ◽  
Early Detection ◽  
Visual Inspection ◽  
Causes Of Death ◽  
Cure Rate ◽  
Blood Disorders ◽  
Detection And Diagnosis ◽  
The Moment ◽  
Early Detection And Diagnosis

Leukemia   disease   is one   of    the   leading   causes   of death   among   human. Its  cure  rate and  prognosis   depends   mainly   on  the  early  detection   and  diagnosis  of   the  disease. At  the  moment, identification  of  blood  disorders  is  through   visual  inspection  of  microscopic  images  by  examining  changes  like  texture, geometry, colour  and   statistical  analysis  of  images . This  project  aims  to  preliminary  of  developing  a  detection  of  leukemia  types  using   microscopic  blood  sample using MATLAB. Images  are  used  as  they  are  cheap  and  do  not  expensive  for testing  and  lab  equipment.

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