Serological investigation of protozoan pathogens (Trypanosoma cruzi, Toxoplasma gondii and Neospora caninum) in opossums from southern Brazil

2021 ◽  
pp. 100546
Author(s):  
L.C. Zitelli ◽  
A. Webster ◽  
A.P. Morel ◽  
K.A. Umeno ◽  
T.C. Padilha ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trypanosoma Cruzi ◽  
Neospora Caninum ◽  
Southern Brazil ◽  
Serological Investigation

scholarly journals Occurrences of anti-Toxoplasma gondiiand anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Vivien Midori Morikawa ◽  
Cristina Kraemer Zimpel ◽  
Igor Adolfo Dexheimer Paploski ◽  
Maria do Carmo Custódio de Souza Hunold Lara ◽  
Eliana Monteforte Cassaro Villalobos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Southern Brazil ◽  
Intermediate Hosts ◽  
Blood Samples ◽  
Ammotragus Lervia ◽  
Barbary Sheep ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Barbary sheep (Ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. Blood samples from 17 Barbary sheep at the Curitiba zoo were collected to evaluate occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (IFAT). Anti-T. gondii and anti-N. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. The present study has shown that Barbary sheep at Curitiba zoo were exposed to T. gondii andN. caninum and therefore may act as intermediate hosts, spreading toxoplasmosis and neosporosis within and between species in shared areas.

scholarly journals Serosurvey for Leishmania spp., Toxoplasma gondii, Trypanosoma cruzi and Neospora caninum in neighborhood dogs in Curitiba-Paraná, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 504-510 ◽  
Author(s):  
Caroline Constantino ◽  
Maysa Pellizzaro ◽  
Edson Ferraz Evaristo de Paula ◽  
Thállitha Samih Wischral Jayme Vieira ◽  
Ana Pérola Drulla Brandão ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trypanosoma Cruzi ◽  
Urban Areas ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Human Movement ◽  
Human Movements ◽  
Public Areas ◽  
Leishmania Spp ◽  
Protozoan Infections

Abstract Neighborhood dogs may act as reservoirs for several zoonotic protozoan infections, particularly in urban areas, thus constituting a potential public health threat. Accordingly, the aim of the present study was to evaluate the exposure of neighborhood dogs to four protozoan pathogens in public areas with high levels of human movement in Curitiba, southern Brazil. Blood samples from 26 neighborhood dogs were screened by means of the indirect immunofluorescent antibody test (IFAT) for Leishmania spp., Toxoplasma gondii, Trypanosoma cruzi and Neospora caninum, and a questionnaire was answered by the respective keeper. A total of 8/26 dogs (30.7%) seroreactive to T. gondii, 3/26 (11.5%) to N. caninum and 2/26 (7.7%) to both were identified. All the samples were seronegative for T. cruzi and Leishmania spp. Pathogen seroreactivity was not associated with the daily human movements or other epidemiological variables investigated (p > 0.05). In conclusion, the low seroprevalence for T. gondii and N. caninum indicated low environmental and food risk for animal infection and the seronegativity for Leishmania spp. and T. cruzi may reflect the absence of these pathogens in urban areas of Curitiba. Moreover, neighborhood dogs may be used as environmental sentinels for the presence of protozoan pathogens and their vectors.

scholarly journals INFECÇÃO POR Toxoplasma gondii, Neospora caninum, Leishmania major E Trypanosoma cruzi EM CÃES DO ESTADO DO PARÁ

2019 ◽  
Vol 20 ◽  
Author(s):  
Giselle Souza da Paz ◽  
Bruna da Silva Colhado ◽  
Mariane Martinez Anton ◽  
Katarine de Souza Rocha ◽  
Daniela Barbosa da Silva ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trypanosoma Cruzi ◽  
Leishmania Major ◽  
Neospora Caninum ◽  
Leishmania Spp

Resumo Realizou-se inquérito sorológico para Toxoplasma gondii, Neospora caninum, Leishmania spp. e Trypanosoma cruzi em cães dos municípios de Castanhal e Belém, no Estado do Pará, e estudou-se a associação entre os animais sororeagentes e fatores como sexo, idade, acesso a rua e procedência dos animais. Foram utilizados 269 cães de diferentes idades, sadios, provenientes de abrigos de animais, canil comercial, Centro de Controle de Zoonoses e domiciliados. Utilizou-se a reação de imunofluorescência indireta (RIFI), com lâminas sensibilizadas de acordo com os seus respectivos antígenos. Das 263 amostras, 100 foram reagentes, com 38,0% de positividade para Toxoplasma gondii, sendo os títulos prevalentes 16UI e 64UI, 15,97% de sororreagentes para anticorpos anti-N. caninum com maior soroprevalência para os títulos 25UI e 50UI. Na pesquisa de anticorpos anti-L. major, 3,42% foram sororreagentes com o título 40UI, sendo o mais prevalente. A pesquisa de anticorpos anti-T. cruzi revelou negatividade em todas as amostras. As variáveis procedência e faixa etária mostraram ser um fator de risco para os animais. Os animais de Belém apresentaram 2,6 vezes mais chances de infecção do que os animais de Castanhal. Os animais na faixa etária de três a sete anos apresentaram 2,27 vezes mais chances de serem sororreagentes do que os cães com até três anos de idade. Não houve associação estatística para as demais variáveis e protozoários pesquisados.

scholarly journals Serological cross-reactivity of Trypanosoma cruzi, Ehrlichia canis, Toxoplasma gondii, Neospora caninum and Babesia canis to Leishmania infantum chagasi tests in dogs

2014 ◽  
Vol 47 (1) ◽  
pp. 105-107 ◽  
Author(s):  
Maurício Franco Zanette ◽  
Valéria Marçal Felix de Lima ◽  
Márcia Dalastra Laurenti ◽  
Claudio Nazaretian Rossi ◽  
Juliana Peloi Vides ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trypanosoma Cruzi ◽  
Leishmania Infantum ◽  
Neospora Caninum ◽  
Cross Reactivity ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
Leishmania Infantum Chagasi

scholarly journals Neospora caninum and/or Toxoplasma gondii Seroprevalence: Vaccination against PCV2 and Muscle Enzyme Activity in Seropositive and Seronegative Pigs

2021 ◽  
Vol 9 (5) ◽  
pp. 1097
Author(s):  
Labrini V. Athanasiou ◽  
Vasileios G. Papatsiros ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Anna Dedousi
Keyword(s):  
Enzyme Activity ◽  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Human Infection ◽  
Porcine Circovirus ◽  
Muscle Enzyme ◽  
Blood Samples ◽  
Zoonotic Pathogen ◽  
Muscle Enzyme Activity ◽  
Immunofluorescence Antibody

Neospora caninum and Toxoplasma gondii affect both humans and animals worldwide. To investigate their seroprevalence and differences in seropositivity between pigs vaccinated and unvaccinated against porcine circovirus 2 (PCV2), as well as differences in muscle enzyme activity between seropositive and seronegative pigs, blood samples were collected from 380 sows. Antibodies against T. gondii and N. caninum were detected by an indirect immunofluorescence antibody (IFA) assay, while the activities of creatine kinase (CK) and aspartate aminotransferase (AST) were biochemically assessed. Out of the 364 sows finally included in the study, 4.4%, 3.5%, and 0.5% were seropositive to T. gondii, N. caninum, or both. A significantly higher percentage of seropositivity against T. gondii and/or N. caninum in PCV2 unvaccinated pigs compared with vaccinated pigs was observed. Increased serum activities of CK and AST were detected in 71.43% and 100% of only against T. gondii (T+) and 63.64% and 90.91% of only against N. caninum (N+) seropositive sows, respectively, and were significantly higher compared to seronegative animals. T. gondii and N. caninum seropositivity, especially in presumed immunocompromised pigs, and the evidence of muscle damage highlight their importance as a zoonotic pathogen and animal model of human infection, respectively.

Transplacental transmission of Neospora caninum to lambs in successive pregnancies of naturally infected sheep in Southern Brazil

2021 ◽  
Vol 23 ◽  
pp. 100537
Author(s):  
Kamila Alcalá Gonçalves Pereira ◽  
Renato Silva de Sousa ◽  
Mary Suzan Varaschin ◽  
Ana Paula Brenner Busch Becker ◽  
Alda Lúcia Gomes Monteiro ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Infected Sheep ◽  
Transplacental Transmission ◽  
Southern Brazil

Cytologic detection of Toxoplasma gondii in the cerebrospinal fluid of a dog and in vitro isolation of a unique mouse-virulent recombinant strain

2021 ◽  
pp. 104063872199668
Author(s):  
Waléria Borges-Silva ◽  
Mariana M. Rezende-Gondim ◽  
Gideão S. Galvão ◽  
Daniele S. Rocha ◽  
George R. Albuquerque ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Toxoplasma Gondii ◽  
Recombinant Strain ◽  
Neospora Caninum ◽  
Clinical Signs ◽  
Species Identity ◽  
Cytologic Examination ◽  
Pcr Rflp ◽  
Tissue Homogenates

Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog.

scholarly journals Occurrence of antibodies against Neospora caninum and/or Toxoplasma gondii in dogs with neurological signs

2011 ◽  
Vol 20 (3) ◽  
pp. 202-206 ◽  
Author(s):  
Nicolle Fridlund Plugge ◽  
Fabiano Montiani Ferreira ◽  
Rosária Regina Tesoni de Barros Richartz ◽  
Adriana de Siqueira ◽  
Rosangela Locatelli Dittrich
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Neurological Diseases ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Metropolitan Region ◽  
Serological Tests ◽  
Stray Dogs ◽  
Neurological Signs ◽  
Significant Difference

This study aimed to evaluate occurrences of antibodies against Neospora caninum and Toxoplasma gondii in dogs with neurological signs. Blood samples from 147 dogs were collected: 127 from owned dogs (attended at the Veterinary Teaching Hospital of the Federal University of Paraná (HV-UFPR) and at private veterinary clinics in the city of Curitiba), and 20 from stray dogs found in Curitiba's metropolitan region. The dogs presented one or more of the following neurological signs: seizures, paresis or paralysis, ataxia, behavioral abnormalities, sensory and somatic disorders and chorioretinitis. The samples were analyzed by means of the indirect fluorescent antibody test (IFAT), at a cutoff dilution of 1:50. Out of the 147 samples obtained, 17 (11.56%) were seropositive for N. caninum, 31 (21.08%) for T. gondii and four (2.72%) for both protozoa. Serum titration on the positive animals showed that 54.83% (17/31) and 41.18% (7/17) had titers > 1:200 against T. gondii and N. caninum, respectively. A significant difference in seropositivity for T. gondii (P = 0.021; OR = 2.87; CI = 1.1 > 2.8 > 7.4) was observed between owned dogs (18.11%) and stray dogs (40%). Inclusion of serological tests for neosporosis and toxoplasmosis is recommended in diagnosing neurological diseases in dogs.

scholarly journals Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

2017 ◽  
Vol 26 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Cesar Augusto Barbosa de Macedo ◽  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
Ana Carolina Miura ◽  
Alessandra Taroda ◽  
Sergio Tosi Cardim ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
High Frequency ◽  
Neospora Caninum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Southern Brazil ◽  
Chain Reaction ◽  
Santa Catarina ◽  
Tissue Samples ◽  
Polymerase Chain

Abstract The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.

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