Inhibition of regrowth of planktonic and biofilm bacteria after peracetic acid disinfection

2022 ◽

Vol 10 (1) ◽

pp. 8-14

Author(s):

N. Eliza ◽

R. Dewanti-Hariyadi ◽

S. Nurjanah

Keyword(s):

Extracellular Matrix ◽

Stainless Steel ◽

Bacillus Cereus ◽

Peracetic Acid ◽

Contact Time ◽

Bacterial Biofilms ◽

Food Industries ◽

Solid Media ◽

Biofilm Bacteria ◽

Ammonium Compounds

Bacillus cereus is known to have the ability to adhere and form biofilms on the surface of stainless steel that causes problems in the food industries. Bacterial biofilms generally can increase resistance to sanitizer treatment. This study aimed to evaluate the ability of peracetic acid-based commercial sanitizer to inactivate B. cereus biofilm on stainless steel (SS) surfaces. Biofilm of B. cereus ATCC 10876 was developed on SS surfaces and treated with 7 commercial peracetic acid-based sanitizers at their recommended dosages. Two sanitizers, i.e. B (peracetic acid and QAC) and F (peracetic acid and acidified water) showing the ability to inactivate B. cereus on solid media at concentration of 200, 400, and 800 ppm were further tested on biofilms with contact times of 1, 3, and 5 minutes. The 48 hours biofilms B. cereus contained 2.78-3.78 CFU/cm2. Both sanitizers B and F had significant effects in inactivating B. cereus biofilm. In general, sanitizer B could reduce more biofilm bacteria at any contact time than sanitizer F. Use of 200 ppm of sanitizer B or F 5 minutes could inactivate 3.04 log CFU/cm2 and 2.68 log CFU/cm2 biofilm, respectively. Exposure of B. cereus biofilm to peracetic acid-based sanitizer resulted in the damage of the extracellular matrix of the biofilms. This study showed that commercial sanitizers containing peracetic acid and quaternary ammonium compounds were effective in inactivating B. cereus biofilms.

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Обеззараживание семян капусты от сосудистого бактериоза

Kartofel` i ovoshi ◽

10.25630/pav.2018.1.17457 ◽

2018 ◽

Author(s):

A.T. Orynbayev ◽

F.S. Dzhalilov

Keyword(s):

Peracetic Acid ◽

Biological Efficiency ◽

Black Rot ◽

Presowing Treatment

Приведены данные двухлетнего исследования по поиску эффективных препаратов для обеззараживания семян капусты от сосудистого бактериоза. Наилучший результат был получен при предпосевной обработке 0,15%-ной надуксусной кислотой. При этом биологическая эффективность составляла 94,1–97,6%, что значительно превышало соответствующий показатель в эталонном варианте – Фитолавин, ВРК, 0,2% (69,0–72,0%). Показано, что заблаговременная обработка семян НУК (за 550 суток до посева) не оказывала достоверного влияния на их лабораторную всхожесть.Presented data are the result of a two-year study aimed at identifying effective preparations for the disinfection of cabbage seeds from black rot. The best result was obtained by presowing treatment with 0.15% peracetic acid. In this case, the biological efficiency was 94.1–97.6%, which significantly exceeded the corresponding indicator in the reference variant – Phytolavin, VRK, 0.2% (69.0–72.0%). The study has not revealed any significant effect of advance treatment of seeds with peracetic acid (550 days before sowing) on their laboratory germination.

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The effect of distribution system biofilm bacteria on copper concentrations in drinking water

Water Science & Technology Water Supply ◽

10.2166/ws.2002.0038 ◽

2002 ◽

Vol 2 (1) ◽

pp. 319-324

Author(s):

M.M. Critchley ◽

N.J. Cromar ◽

N. McClure ◽

H.J. Fallowfield

Keyword(s):

Public Health ◽

Drinking Water ◽

Distribution System ◽

Xanthomonas Maltophilia ◽

Biofilm Bacteria ◽

Rhodococcus Sp

This study investigated the potential for distribution system biofilm bacteria to elevate copper concentrations in drinking water. Biofilms were sampled from household copper reticulation pipes and grown on R2A agar. Laboratory coupon experiments were used to determine the effect of single isolate biofilms on aqueous copper concentrations. The majority of biofilm bacteria did not affect copper concentrations in comparison to sterile controls. However, several bacteria including Acidovorax delafieldii, Cytophaga johnsonae and Micrococcus kristinae were shown to significantly elevate copper concentrations in drinking water. In contrast, the bacteria Rhodococcus sp. and Xanthomonas maltophilia were shown to significantly decrease copper levels in comparison to controls. The significance of biofilm bacteria to increase copper concentrations in drinking water has implications for public health by increasing concentrations to levels toxic to humans.

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Multiple interactions in riverine biofilms - surfactant adsorption, bacterial attachment and biodegradation

Water Science & Technology ◽

10.2166/wst.1995.0015 ◽

1995 ◽

Vol 31 (1) ◽

pp. 61-70 ◽

Cited By ~ 6

Author(s):

Graham F. White

Keyword(s):

Organic Pollutants ◽

Bacterial Attachment ◽

Solid Surfaces ◽

Sediment Surface ◽

Liquid Interfaces ◽

Polluted Soils ◽

Biofilm Bacteria ◽

The Impact ◽

Multiple Interactions

Many organic pollutants, especially synthetic surfactants, adsorb onto solid surfaces in natural and engineered aquatic environments. Biofilm bacteria on such surfaces make major contributions to microbial heterotrophic activity and biodegradation of organic pollutants. This paper reviews evidence for multiple interactions between surfactants, biodegradative bacteria, and sediment-liquid interfaces. Biodegradable surfactants e.g. SDS, added to a river-water microcosm were rapidly adsorb to sediment surface and stimulated the indigenous bacteria to attach to the sediment particles. Recalcitrant surfactants and non-surfactant organic nutrients did not stimulate attachment Attachment of bacteria was maximal when biodegradation was fastest, and was reversed when biodegradation was complete. Dodecanol, the primary product of SDS-biodegradation, markedly stimulated attachment. When SDS was added to suspensions containing sediment and either known degraders or known non-degraders, only the degraders became attached, and attachment accelerated surfactant biodegradation to dodecanol. These cyclical cooperative interactions have implications for the design of biodegradability-tests, the impact of surfactant adjuvants on biodegradability of herbicides/pesticides formulated with surfactants, and the role of surfactants used to accelerate bioremediation of hydrocarbon-polluted soils.

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Detachment of biofilm bacteria due to variations in nutrient supply

Water Science & Technology ◽

10.2166/wst.1998.0624 ◽

1998 ◽

Vol 37 (4-5) ◽

pp. 211-214 ◽

Cited By ~ 17

Author(s):

Linda K. Sawyer ◽

Slawomir W. Hermanowicz

Keyword(s):

Phase Contrast ◽

Digital Image Analysis ◽

Specific Growth ◽

Nutrient Supply ◽

Surface Shear Stress ◽

Surface Shear ◽

Nutrient Limitations ◽

Contrast Microscopy ◽

Biofilm Bacteria ◽

Observable Parameter

Growth and detachment rates of an environmental isolate of Aeromonas hydrophila attached to a surface were determined under varying nutrient supply conditions in a complex medium. Growth and detachment of cells were observed in real time using phase contrast microscopy in glass parallel plate flow chambers. Surface shear stress was controlled in all experiments at 3 N m−2. Images were taken every 15 min. Digital image analysis was used to determine specific growth and detachment rates. An observable parameter proportional to the nutrient depletion at the surface due to transfer limitations was used to indicate nutrient limitations. Specific detachment rates increased as the depletion parameter increased, indicating that nutrient limitations cause this bacterium to detach at greater rates.

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Microbial Biofilms and Biotechnology – Some Perceptions

Current Biotechnology ◽

10.2174/2211550109999201026091512 ◽

2020 ◽

Vol 09 ◽

Author(s):

Subba Rao Toleti

Keyword(s):

Tissue Culture ◽

Synthetic Biology ◽

Industrial Effluents ◽

Aquatic Environments ◽

Biochemical Processes ◽

Microbial Biofilms ◽

Biofilm Bacteria ◽

Prophylactic Vaccines ◽

Design Construction

: The review is an attempt to introduce the readers in brief about biofilms and their implications as well as some new perceptions in biotechnology. Biofilms are adherent microbial communities, which are developed on submerged surfaces in aquatic environments. Biofilms play a significant role in exopolymer production, material deterioration and also cause harmful infections. Further, the role of corrosion causing biofilm bacteria in deterioration of different materials, microbial biofilms and their enzymatic processes in reducing the toxicity of pollutants in industrial effluents are elaborated, along with clean technologies for wastewater treatment. Biotechnology is defined as any technological application that uses biological systems to synthesize or modify products or processes. The applications include biochemical processes, medical care, cell and tissue culture as well as synthetic biology and others. Synthetic biology details about the design, construction of new biological components and systems for useful purposes. Finally, to overcome the limitations that are inherent to the use of cellular host’s, cell-free systems as critical platforms for synthetic biology applications. This mini-review also mentions about new diagnostic products based on enzymes, monoclonal antibodies and engineered proteins as well as novel prophylactic vaccines.

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