scholarly journals Aggregation and Size Attributes Analysis of Unadsorbed and Adjuvant-Adsorbed Antigens using a Multispectral Imaging Flow Cytometer Platform

2021 ◽  
Author(s):  
Christopher H. Choy ◽  
Liwei He ◽  
David Tulumello ◽  
Beata Gajewska ◽  
Mauricio R. Terebiznik ◽  
...  
Keyword(s):  
Multispectral Imaging ◽  
Flow Cytometer

Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer

2006 ◽  
Vol 317 (1-2) ◽  
pp. 90-99 ◽  
Author(s):  
Paul V. Beum ◽  
Margaret A. Lindorfer ◽  
Brian E. Hall ◽  
Thaddeus C. George ◽  
Keith Frost ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
B Cells ◽  
Multispectral Imaging ◽  
Flow Cytometer

scholarly journals Distinguishing modes of cell death using the ImageStream® multispectral imaging flow cytometer

2004 ◽  
Vol 59A (2) ◽  
pp. 237-245 ◽  
Author(s):  
Thaddeus C. George ◽  
David A. Basiji ◽  
Brian E. Hall ◽  
David H. Lynch ◽  
William E. Ortyn ◽  
...  
Keyword(s):  
Cell Death ◽  
Multispectral Imaging ◽  
Flow Cytometer

Loss of Thrombin-Induced Ca2+ Mobilization in a Subpopulation of Platelets during Storage

1991 ◽  
Vol 66 (03) ◽  
pp. 350-354 ◽  
Author(s):  
Rob Fijnheer ◽  
Christa H E Homburg ◽  
Berend Hooibrink ◽  
Martine N Boomgaard ◽  
Dirk de Korte ◽  
...  
Keyword(s):  
Human Platelets ◽  
Flow Cytometer ◽  
Maximal Concentration ◽  
Gradual Decline ◽  
Platelet Storage ◽  
Functional Defect ◽  
Total Fluorescence ◽  
Induced Changes

SummaryThrombin-induced changes in cytosolic free Ca2+ ([Ca2+]i) were studied in human platelets that had been stored for up to 6 days. Changes in [Ca2+]i were measured with Indo-1-loaded platelets and quantitated with two different methods: (i) measurement of the changes in total fluorescence; (ii) measurement of the [Ca2+]i changes in individual platelets in a flow cytometer, allowing the detection of non-responding platelets. The maximal concentration of [Ca2+]i after stimulation with 0.5 U of thrombin/ml decreased from 544 ± 58 nM (mean ± SEM, n = 6) on day 0, to 276 ± 9 nM on day 3 and to 203 ± 23 nM on day 6. The percentage of platelets responding to 0.5 U of thrombin/ml declined from 90 ± 2% on day 0 to 72 ± 4% on day 3, and to 47 ± 8% on day 6. Nevertheless, also the responding platelets showed a decreased rise in [Ca2+]i.The study shows that during platelet storage a decrease in the rise in [Ca2+]i upon thrombin stimulation occurs. This decrease is partly due to the formation of a subpopulation of platelets that is completely unresponsive and partly due to a decreased responsiveness in the remainder of the platelets; it is not due to a gradual decline in [Ca2+]i rise in all platelets. This phenomenon provides new insight in the functional defect of stored platelets.

Multispectral Imaging to define morpho-molecular classes of human HCC

2020 ◽  
Author(s):  
T Ritz ◽  
J Baues ◽  
O Krenkel ◽  
P Schirmacher ◽  
T Longerich
Keyword(s):  
Multispectral Imaging

Impact of the New Beckman Coulter Cytomics FC 500 5-Color Flow Cytometer on a Regional Flow Cytometry Clinical Laboratory Service

2004 ◽  
Vol 10 (2) ◽  
pp. 102-108 ◽  
Author(s):  
J. Luider ◽  
M. Cyfra ◽  
P. Johnson ◽  
I. Auer
Keyword(s):  
Flow Cytometry ◽  
Clinical Laboratory ◽  
Flow Cytometer ◽  
Color Flow ◽  
Laboratory Service ◽  
Regional Flow

Rapid and Real-time Determination of Polyphenols in Gongju (Chrysanthemum morifolium Ramat.) at Different Storage Periods by Multispectral Imaging System

2020 ◽  
Vol 26 (6) ◽  
pp. 701-707
Author(s):  
He Yang ◽  
Wei Liu ◽  
Wei Qu ◽  
Fangbin Wang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Chrysanthemum Morifolium ◽  
Time Determination

Identification of antibiotics in meat. Flow cytometry capabilities

Vsyo o myase ◽  
2020 ◽  
pp. 56-60
Author(s):  
Zayko E.V. ◽  
◽  
Kuznetsova O.A. ◽  
Bataeva D.S. ◽  
Grudistova M.A. ◽  
...  
Keyword(s):  
Raw Materials ◽  
Animal Husbandry ◽  
Flow Cytometer ◽  
Poultry Meat ◽  
Meat Processing ◽  
Beta Lactams ◽  
Antimicrobial Substances ◽  
Chemotherapy Drugs ◽  
Processing Plants ◽  
Meat Samples

The problem of the uncontrolled use of antibiotics currently remains unresolved. Step-by-step monitoring of meat using modern methods will reduce the risk of using contaminated meat raw materials for food production. Qualitative monitoring will identify samples containing residual antimicrobial substances. The use of methods for identifying groups of antibiotics will help narrow the search for antibiotics by expensive chromatographic methods. A study was carried out of beef, pork and poultry meat, which is used in meat processing plants in the production of raw smoked sausages, using two methods. At the first stage, using a qualitative microbiological method, the raw meat was evaluated for the presence of antimicrobial substances, then their group was determined using a NovoCyte flow cytometer. According to the results of a study on a flow cytometer, it was found that out of 10 groups of antibiotics that can be determined by the tested method, the group of lincosamides was not found in all meat samples. The most common groups of chemotherapy drugs in pork were sulfonamides – 29.6 %, tetracycline group – 18.5 % and beta-lactams – 14.8 %, and in beef aminoglycosides – 36.7 %, phenicols – 30 % and beta-lactams – 13.3 %. In poultry meat samples, the most common were sulfonamides – 23.2 %, fenicols – 23.2 %, and beta-lactams – 16 %. Five groups of antibiotics were found in all studied types of meat: fenicols, β-lactams, macrolides, polypeptide antibiotics, and quinolones. This indicates their widespread use in animal husbandry and poultry farming.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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