scholarly journals Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry

2022 ◽  
Vol 3 (1) ◽  
pp. 101065
Author(s):  
Fernando Janczur Velloso ◽  
Ekta Kumari ◽  
Krista D. Buono ◽  
Michelle J. Frondelli ◽  
Steven W. Levison
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Progenitor Cell ◽  
Multicolor Flow Cytometry ◽  
Progenitor Cell Proliferation

c-Myb Is Required for Neural Progenitor Cell Proliferation and Maintenance of the Neural Stem Cell Niche in Adult Brain

Stem Cells ◽  
2008 ◽  
Vol 26 (1) ◽  
pp. 173-181 ◽  
Author(s):  
Jordane Malaterre ◽  
Theo Mantamadiotis ◽  
Sebastian Dworkin ◽  
Sally Lightowler ◽  
Qing Yang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Adult Brain ◽  
Progenitor Cell Proliferation ◽  
Cell Niche

scholarly journals Phosphoserine Phosphatase Is Expressed in the Neural Stem Cell Niche and Regulates Neural Stem and Progenitor Cell Proliferation

Stem Cells ◽  
2007 ◽  
Vol 25 (8) ◽  
pp. 1975-1984 ◽  
Author(s):  
Ichiro Nakano ◽  
Joseph D. Dougherty ◽  
Kevin Kim ◽  
Ivan Klement ◽  
Daniel H. Geschwind ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Progenitor Cell ◽  
Phosphoserine Phosphatase ◽  
Neural Stem Cell Niche ◽  
Progenitor Cell Proliferation ◽  
Cell Niche

Contact-mediated inhibition of human haematopoietic progenitor cell proliferation may be conferred by stem cell antigen, CD34

2000 ◽  
Vol 1 (2) ◽  
pp. 77-86 ◽  
Author(s):  
M Y Gordon ◽  
S B Marley ◽  
R J Davidson ◽  
F H Grand ◽  
J L Lewis ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Progenitor Cell ◽  
Haematopoietic Progenitor Cell ◽  
Cell Antigen ◽  
Progenitor Cell Proliferation

ID: 22: DIFFERENTIAL ACTIONS OF ESTROGEN RECEPTOR α AND β VIA NON-GENOMIC SIGNALING IN HUMAN PROSTATE STEM-PROGENITOR CELLS

2016 ◽  
Vol 64 (4) ◽  
pp. 933-933
Author(s):  
S Majumdar ◽  
JC Rinaldi ◽  
T Gauntner ◽  
L Xie ◽  
W Hu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Cell Line ◽  
Progenitor Cells ◽  
Progenitor Cell ◽  
Mapk Pathway ◽  
Human Prostate ◽  
Stem Cell Markers ◽  
Self Renewal ◽  
Progenitor Cell Proliferation

Genomic signaling via estrogen receptors (ER) has been widely studied and implicated as the main ER signaling pathway in prostate development and carcinogenesis. Non-genomic ER signaling has also been reported in prostate epithelium although down-stream cascades have not been clarified. Our lab has recently identified ERs in human prostate epithelial stem/progenitor cells and shown that that 17β-estradiol (E2) can stimulate stem cell symmetric self-renewal and progenitor cell proliferation. In this study we interrogate non-genomic membrane initiated ER signaling in this prostate stem/progenitor cell population. Human prostate stem-progenitor cells were enriched from primary prostate epithelial cell cultures (PrEC) of young, disease-free donors using a 3D prostasphere (PS) model as previously described. Cells were labeled using ERα or ERβ antibodies along with prostate stem cell markers CD49f and TROP2 followed by triple channel FACS to quantify ERα+/ERβ+ cell numbers. To explore ERα, the benign human prostate stem cell line WPE with extremely low levels of ERα and ERβ, was stably transfected with a lentiviral-ERα expression vector. The human prostate cancer stem-like cell line HuSLC (ERβ++, ERα−) was utilized to interrogate ERb actions. Cells were exposed to 10 nM estradiol (E2) over a 15 to 60 minute time course +/− ICI 182,870 (ICI), an ERα/β antagonist. FACS analysis of day 7 PS cells labeled for ERα or ERβ revealed 66% of day 7 PS cells as ERα+ and 40% as ERβ+. Among ERα or ERβ positive PS cells, 4% were Trop2+/CD49fhigh (stem-like cells) and 10–12% were Trop2+/CD49fmedium (early stage progenitor cells). PS exposed to 10 nM E2 showed sequential phosphorylation of Src, Erk1/2, p38, Akt and NFκB (p65) over 60 minutes. Phosphorylation of up-and downstream targets (EGFR, Jnk, GSK 3α/β, p70 S6 kinase, PRAS40, MSK1/2) was also seen using a phospho-kinase array. Furthermore, phosphorylation of ERα at S167 was noted over 60 min of E2 exposure enabling enhancement of genomic ERα transactivational activity in a feed-forward manner. ICI attenuated Akt and Erk1/2 phosphorylation, confirming membrane bound ERs are involved in downstream signaling. E2 treatment of HuSLCs showed phosphorylation of Erk1/2 but not Akt, indicating that ERβ signals exclusively through the MAPK pathway in these cells. Conversely, E2 treatment of WPE-stem cells overexpressing ERα resulted in robust phosphorylation of Akt but lower levels of Erk1/2 phosphorylation suggesting that Akt activation may be more reliant on ERα signaling. To identify pathway specific roles, specific inhibitors were added to PS cultures. PS treated with LY294002 (Akt inhibitor) for 7 days attenuated the E2-mediated increase in PS number and size. Inhibition of the NFκB downstream of the Akt pathway by IKK VII (IKK inhibitor) blocked p65 phosphorylation, abrogated the E2-induced increase in stem cell symmetric self-renewal and blunted E2 stimulation of progenitor cell proliferation. Analysis of PS cyclin mRNA levels revealed a G1 arrest of progenitor cells upon IKK inhibition suggesting an essential role of NFκB in progenitor cell amplification. MAPK pathway inhibition with U0126(Erk1/2 inhibitor) resulted in an attenuation of the E2-mediated increase in PS number and size and an increase stem cell symmetric self-renewal suggesting that MAPK pathway activation promotes commitment to stem and progenitor cell expansion. Taken together, the present findings reveal that human prostate stem-progenitor cells express both ERα and ERβ which differentially activate different signaling cascades originating at the membrane. These signaling events may lead to unique downstream actions that influence prostate stem-progenitor cell proliferation as well as lineage commitment decisions.

scholarly journals CXCR4/CXCL12 signaling impacts enamel progenitor cell proliferation and motility in the dental stem cell niche

2015 ◽  
Vol 362 (3) ◽  
pp. 633-642 ◽  
Author(s):  
Tamaki Yokohama-Tamaki ◽  
Keishi Otsu ◽  
Hidemitsu Harada ◽  
Shunichi Shibata ◽  
Nobuko Obara ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Progenitor Cell ◽  
Progenitor Cell Proliferation ◽  
Cell Niche ◽  
Dental Stem Cell

Increasing Progenitor Cell Proliferation in the Sub-Ventricular Zone: A Therapeutic Treatment for Progressive Multiple Sclerosis?

2020 ◽  
Vol 14 ◽  
Author(s):  
Tahir Sulehria ◽  
Adrian M. Corbett ◽  
Neelima Sharma ◽  
Devipriyanka Nagarajan ◽  
Amani Abushamma ◽  
...  
Keyword(s):  
Gender Differences ◽  
Cell Proliferation ◽  
Ascorbic Acid ◽  
Stem Cell ◽  
Gender Difference ◽  
Progenitor Cell ◽  
Ventricular Zone ◽  
Female Rats ◽  
Stem Cell Proliferation ◽  
Progenitor Cell Proliferation

Introduction: The purpose of this study was to determine if pharmacological treatment could increase progenitor cell proliferation in the Sub-ventricular Zone of aged rats. Previous work had shown that increasing progenitor cell proliferation in this region correlated well (R2=0.78; p= 0.0007) with functional recovery in a damaged corpus callosum (white matter tract), suggesting that progenitor cell proliferation results in oligodendrocytes in this region. Methods: 10 month old male and female Sprague Dawley rats were fed the drugs for 30 days in cookie dough, then immunocytochemistry was performed on coronal brain sections, using Ki67 labeling to determine progenitor cell proliferation. Results: Female rats showed low endogenous (control) progenitor cell proliferation, significantly different from male rats (P<0.0001), at this age. Ascorbic Acid (20 mg/kg, daily for 30 days) increased progenitor cell proliferation overall, but maintained the innate gender difference in stem cell proliferation (P=0.001). Prozac (5 mg/kg, daily for 30 days) increased progenitor cell proliferation for females but decreased stem cell proliferation for males, again showing a gender difference (P<0.0001). Simvastatin (1 mg/kg for 30 days) also increased progenitor cell proliferation in females and decreased progenitor cell proliferation in males, leading to a significant gender difference. Discussion: The three drug combination (fluoxetine, simvastatin, and ascorbic acid, patent # 9,254,281) led to ~ 4 fold increase in progenitor cell proliferation in females, while male progenitor cell proliferation was highest with 50 mg/kg ascorbic acid. However, the ascorbic acid increase in proliferation appears to be only on the sides of the ventricles, which is not the region that normally gives rise to oligodendrocytes. Conclusion: There are innate gender differences in progenitor cell proliferation at the Sub-Ventricular Zone at middle age in rats, possibly due to loss of estrogen in females. We also see notable gender differences in progenitor cell proliferation in the Sub ventricular Zone in the response to common drugs, such as fluoxetine, simvastatin and Vitamin C (ascorbic acid).

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