Cell cycle independent role of Cyclin E during neural cell fate specification in Drosophila is mediated by its regulation of Prospero function

Neural cell fate specification is a multistep process in which stem cells undergo sequential changes in states, giving rise to particular lineages such as neurons and astrocytes. This process is accompanied by dynamic changes of chromatin and in transcription, thereby orchestrating lineage-specific gene expression programs. A pressing question is how these events are interconnected to sculpt cell fate. We show that altered chromatin due to loss of the chromatin remodeler Chd5 causes neural stem cell activation to occur ahead of time. This premature activation is accompanied by transcriptional derepression of ribosomal subunits, enhanced ribosome biogenesis, and increased translation. These untimely events deregulate cell fate decisions, culminating in the generation of excessive numbers of astrocytes at the expense of neurons. By monitoring the proneural factor Mash1, we further show that translational control is crucial for appropriate execution of cell fate specification, thereby providing new insight into the interplay between transcription and translation at the initial stages of neurogenesis.

Download Full-text

Genes necessary for C. elegans cell and growth cone migrations

Development ◽

10.1242/dev.124.9.1831 ◽

1997 ◽

Vol 124 (9) ◽

pp. 1831-1843 ◽

Cited By ~ 20

Author(s):

W.C. Forrester ◽

G. Garriga

Keyword(s):

Cell Fate ◽

Single Gene ◽

Growth Cones ◽

Cell Fate Specification ◽

Fate Specification ◽

C Elegans ◽

Final Destination ◽

Multiple Cell

The migrations of cells and growth cones contribute to form and pattern during metazoan development. To study the mechanisms that regulate cell motility, we have screened for C. elegans mutants defective in the posteriorly directed migrations of the canal-associated neurons (CANs). Here we describe 14 genes necessary for CAN cell migration. Our characterization of the mutants has led to three conclusions. First, the mutations define three gene classes: genes necessary for cell fate specification, genes necessary for multiple cell migrations and a single gene necessary for final positioning of migrating cells. Second, cell interactions between the CAN and HSN, a neuron that migrates anteriorly to a position adjacent to the CAN, control the final destination of the HSN cell body. Third, C. elegans larval development requires the CANs. In the absence of CAN function, larvae arrest development, with excess fluid accumulating in their pseudocoeloms. This phenotype may reflect a role of the CANs in osmoregulation.

Download Full-text

Stem Cell Fate Specification: Role of Master Regulatory Switch Transcription Factor PU.1 in Differential Hematopoiesis

Stem Cells and Development ◽

10.1089/scd.2005.14.140 ◽

2005 ◽

Vol 14 (2) ◽

pp. 140-152 ◽

Cited By ~ 31

Author(s):

Gurudutta U. Gangenahalli ◽

Pallavi Gupta ◽

Daman Saluja ◽

Yogesh K. Verma ◽

Vimal Kishore ◽

...

Keyword(s):

Transcription Factor ◽

Stem Cell ◽

Cell Fate ◽

Cell Fate Specification ◽

Stem Cell Fate ◽

Fate Specification

Download Full-text

The role of the Rx homeobox gene in retinal progenitor proliferation and cell fate specification

Mechanisms of Development ◽

10.1016/j.mod.2018.04.003 ◽

2018 ◽

Vol 151 ◽

pp. 18-29 ◽

Cited By ~ 6

Author(s):

H.M. Rodgers ◽

V.J. Huffman ◽

V.A. Voronina ◽

M. Lewandoski ◽

P.H. Mathers

Keyword(s):

Cell Fate ◽

Homeobox Gene ◽

Cell Fate Specification ◽

Fate Specification ◽

Retinal Progenitor

Download Full-text

T1728 Role of ADAM10 Signaling in Cell Proliferation and Cell Fate Specification is Conserved Between the Developing and Adult Intestine

Gastroenterology ◽

10.1016/s0016-5085(10)62606-6 ◽

2010 ◽

Vol 138 (5) ◽

pp. S-566 ◽

Cited By ~ 2

Author(s):

Yu-Hwai Tsai ◽

Kelli L. VanDussen ◽

Howard C. Crawford ◽

Linda C. Samuelson ◽

Peter J. Dempsey

Keyword(s):

Cell Proliferation ◽

Cell Fate ◽

Cell Fate Specification ◽

Fate Specification

Download Full-text

Atypical cell cycle control over neural cell fate

Cell Cycle ◽

10.4161/15384101.2014.962849 ◽

2014 ◽

Vol 13 (19) ◽

pp. 2987-2987

Author(s):

Dorota Lubanska ◽

Lisa A Porter

Keyword(s):

Cell Cycle ◽

Cell Fate ◽

Cell Cycle Control ◽

Neural Cell ◽

Atypical Cell ◽

Neural Cell Fate

Download Full-text

Abdominal-A mediated repression of Cyclin E expression during cell-fate specification in the Drosophila central nervous system

Mechanisms of Development ◽

10.1016/j.mod.2009.09.008 ◽

2010 ◽

Vol 127 (1-2) ◽

pp. 137-145 ◽

Cited By ~ 13

Author(s):

Ramakrishnan Kannan ◽

Christian Berger ◽

Sudharani Myneni ◽

Gerhard M. Technau ◽

L.S. Shashidhara

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Cell Fate ◽

Cyclin E ◽

Cell Fate Specification ◽

Fate Specification

Download Full-text

Role of Amyloid Precursor Protein (APP) and Its Derivatives in the Biology and Cell Fate Specification of Neural Stem Cells

Molecular Neurobiology ◽

10.1007/s12035-018-0914-2 ◽

2018 ◽

Vol 55 (9) ◽

pp. 7107-7117 ◽

Cited By ~ 17

Author(s):

Raquel Coronel ◽

Adela Bernabeu-Zornoza ◽

Charlotte Palmer ◽

Mar Muñiz-Moreno ◽

Alberto Zambrano ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Amyloid Precursor Protein ◽

Cell Fate ◽

Precursor Protein ◽

Cell Fate Specification ◽

Fate Specification

Download Full-text

Single cell transplantation reveals interspecific cell communication in Drosophila chimeras

Development ◽

10.1242/dev.109.4.821 ◽

1990 ◽

Vol 109 (4) ◽

pp. 821-832 ◽

Cited By ~ 2

Author(s):

T. Becker ◽

G.M. Technau

Keyword(s):

Cell Cycle ◽

Single Cell ◽

Cell Size ◽

Cell Fate ◽

Cell Communication ◽

Host Tissue ◽

Cell Fate Specification ◽

Fate Specification ◽

Common Strategy ◽

Invertebrate Development

Cell-cell communication is not only a common strategy for cell fate specification in vertebrates, but plays important roles in invertebrate development as well. We report here on experiments testing the compatibility of mechanisms specifying cell fate among six different Drosophila species. Following interspecific transplantation, the development of single ectodermal cells was traced in order to test their abilities to proliferate and differentiate in a heterologous environment. Despite considerable differences in cell size and length of cell cycle among some of the species, the transplants gave rise to fully differentiated clones that were integrated into the host tissue. Clones comprised cells of epidermal and/or neural histotypes, indicating that mechanisms mediating the epidermal/neural dichotomy in the ectoderm are conserved between the species. Cells of the neural lineages differentiated into neurones, glia, or both. Moreover, heterologous neurones sent out axons that followed major pathways along nerves and within the neuropile, demonstrating their ability to recognize positional cues in the heterologous CNS of the host.

Download Full-text