scholarly journals Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli

2016 ◽  
Vol 36 ◽  
pp. 1-9 ◽  
Author(s):  
Nadine Ladkau ◽  
Miriam Assmann ◽  
Manfred Schrewe ◽  
Mattijs K. Julsing ◽  
Andreas Schmid ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methyl Ester ◽  
Acid Methyl Ester ◽  
Efficient Production ◽  
Dodecanoic Acid ◽  
Acid Methyl ◽  
Nylon 12 ◽  
Engineered Escherichia Coli

scholarly journals Outer Membrane Protein AlkL Boosts Biocatalytic Oxyfunctionalization of Hydrophobic Substrates in Escherichia coli

2012 ◽  
Vol 78 (16) ◽  
pp. 5724-5733 ◽  
Author(s):  
Mattijs K. Julsing ◽  
Manfred Schrewe ◽  
Sjef Cornelissen ◽  
Inna Hermann ◽  
Andreas Schmid ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methyl Ester ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Acid Methyl Ester ◽  
Dodecanoic Acid ◽  
Content Type ◽  
E Coli ◽  
Acid Methyl

ABSTRACTThe outer membrane of microbial cells forms an effective barrier for hydrophobic compounds, potentially causing an uptake limitation for hydrophobic substrates. Low bioconversion activities (1.9 U gcdw−1) have been observed for the ω-oxyfunctionalization of dodecanoic acid methyl ester by recombinantEscherichia colicontaining the alkane monooxygenase AlkBGT ofPseudomonas putidaGPo1. Using fatty acid methyl ester oxygenation as the model reaction, this study investigated strategies to improve bacterial uptake of hydrophobic substrates. Admixture of surfactants and cosolvents to improve substrate solubilization did not result in increased oxygenation rates. Addition of EDTA increased the initial dodecanoic acid methyl ester oxygenation activity 2.8-fold. The use of recombinantPseudomonas fluorescensCHA0 instead ofE. coliresulted in a similar activity increase. However, substrate mass transfer into cells was still found to be limiting. Remarkably, the coexpression of thealkLgene ofP. putidaGPo1 encoding an outer membrane protein with so-far-unknown function increased the dodecanoic acid methyl ester oxygenation activity of recombinantE. coli28-fold. In a two-liquid-phase bioreactor setup, a 62-fold increase to a maximal activity of 87 U gcdw−1was achieved, enabling the accumulation of high titers of terminally oxyfunctionalized products. Coexpression ofalkLalso increased oxygenation activities toward the natural AlkBGT substrates octane and nonane, showing for the first time clear evidence for a prominent role of AlkL in alkane degradation. This study demonstrates that AlkL is an efficient tool to boost productivities of whole-cell biotransformations involving hydrophobic aliphatic substrates and thus has potential for broad applicability.

Production of 12-hydroxy dodecanoic acid methyl ester using a signal peptide sequence-optimized transporter AlkL and a novel monooxygenase

2019 ◽  
Vol 291 ◽  
pp. 121812 ◽  
Author(s):  
Hee-Wang Yoo ◽  
Joonwon Kim ◽  
Mahesh D. Patil ◽  
Beom Gi Park ◽  
Sung-yeon Joo ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Signal Peptide ◽  
Acid Methyl Ester ◽  
Peptide Sequence ◽  
Dodecanoic Acid ◽  
Signal Peptide Sequence ◽  
Acid Methyl

Determination of Biomedical Constituents of 350°C Pyrolyzate from Benzene/Ethanol Extractives of Old Bark from Cinnamomum Camphora Trunk

2011 ◽  
Vol 230-232 ◽  
pp. 935-939
Author(s):  
Wei He ◽  
Dang Quan Zhang ◽  
Qi Mei Liu ◽  
Kuan Peng
Keyword(s):  
Methyl Ester ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Value Added ◽  
Acid Ethyl Ester ◽  
Dodecanoic Acid ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Analytical Result ◽  
Main Components

The extractives of C. camphora old bark were used to cure some special diseases; however, the biomedical constituents of extractives of C. camphora old bark are still not completely explained. Therefore, 350 °C-based pyrolysis- GC/MS technology was used to identify the top value-added biomedical constituents of old bark-based benzene/ethanol extractives from C. camphora trunk. 74 chemical constituents representing 99.17% were identified from 86 peaks. The main components are as: Dodecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester (15.717%), Octacosanoic acid, methyl ester (10.357%), Abietic acid (8.483%), Heptacosane (5.678%), Ethyl alcohol (5.282%), Hexadecanoic acid, 2-hydroxy-, methyl ester (3.365%), Hexacosanoic acid, methyl ester (2.936%), 1,2-Benzenedicarboxylic acid, butyl 2-methylpropyl ester (2.928%), 2-Methoxy-4-vinylphenol (2.692%), Hexadecanoic acid, ethyl ester (2.312%), Hexacosane (2.076%), Tetracosanoic acid, methyl ester (2.013%), etc. The analytical result showed that the 350 °C pyrolyzate of benzene/ethanol extractives from C. camphora old bark is abundant in biomedical constituents, and also contains other bioactive components, which can be used as top value-added materials of high-grade cosmetic, food, spice and chemical solvents.

scholarly journals Evaluation of Colilert-18 for Detection of Coliforms and Eschericha coli in Subtropical Freshwater

2004 ◽  
Vol 70 (2) ◽  
pp. 1242-1244 ◽  
Author(s):  
Kuo-Kuang Chao ◽  
Chen-Ching Chao ◽  
Wei-Liang Chao
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
False Positive ◽  
Acid Methyl Ester ◽  
E Coli ◽  
Acid Methyl

ABSTRACT The accuracy of Colilert-18 as a test for coliforms and Escherichia coli in subtropical freshwater was evaluated by using API 20E strips and fatty acid methyl ester analysis. The false-positive and -negative rates of detection were 7.4 and 3.5%, respectively, for E. coli and 9.6 and 6.3%, respectively, for coliforms.

scholarly journals GC-MS Analysis of Active and Applicable Compounds in Methanol Extract of Sweet Star Fruit (Averrhoa carambola L.) Leaves

2021 ◽  
Vol 28 (1) ◽  
pp. 12
Author(s):  
Ni Putu Adriani Astiti ◽  
Yan Ramona
Keyword(s):  
Methyl Ester ◽  
Methanol Extract ◽  
Acid Methyl Ester ◽  
Acid Ethyl Ester ◽  
Methanol Solution ◽  
Dodecanoic Acid ◽  
Octadecanoic Acid ◽  
Hexadecanoic Acid ◽  
Averrhoa Carambola ◽  
Acid Methyl

The leaves of star fruit plants (Averrhoa carambola) have been traditionally used to cure many diseases, such as hypertension and fever. Besides, the leaves of this plant have also widely been used as the main raw material in lawar (a Balinese traditional food) making. In this research, the methanol extract of this plant leaves was analyzed and identified for active compound contents. The main objective of this research was to investigate types of possible active and applicable compounds contained in such leaves, previously extracted/macerated in methanol solution. Analysis was conducted by applying GC-MS instrumentation using methanol solution. The results showed that 10 possible active and applicable compounds (Butane, 1,1-diethoxy-3 methyl-(CAS)1.1-DII, Dodecanoic acid, methyk ester(CAS) methyl, Dodecanoic acid, methyl ester (CAS) Ethyl Laun, Pentadecanoic acid ethyl ester, Hexadecanoic acid methyl ester (CAS) Methyl pa, OCTADECA 9.12 DIENOIC ACID METHYL, 9-Octadecenoic acid methyl ester (E)-(CAS), Octadecanoic acid methyl ester, and (E) 9-Octadecanoic acid ethyl ester) were identified in methanol extract of such leaves. Among those compounds, Butane, 1,1-diethoxy-3-methyl and Hexadecanoic acid, methyl ester was two most abundance constituents with percentage of peak areas of 35.67% and 26.93%, respectively.

scholarly journals Comparative Study of Antimicrobial Potentials of Seeed Oils of Jatropha Curcas and Tamarindus Indica

2019 ◽  
pp. 37-49
Author(s):  
Bilya Abdu
Keyword(s):  
Escherichia Coli ◽  
Methyl Ester ◽  
Jatropha Curcas ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Fragmentation Pattern ◽  
Tamarindus Indica ◽  
Octadecanoic Acid ◽  
Acid Methyl ◽  
Jatropha Seeds

Oils of the dry seeds of Jatropha curcas and Tamarindus indica plants grown in Nigeria were obtained by maceration using n-Hexane. GC/MS analysis was performed using a Shimadzu QP2010plus series gas chromatography coupled with Shimadzu QP2010 mass spectroscopy detector. Characterization of constituents was done by comparison with the MS library (NIST) and further confirmed by interpreting the observed fragmentation pattern. Comparison of the mass spectra with the database on MS library revealed about 90-95% match. The antimicrobial screening of the oils were also performed for oils from the species: Jatropha curcas (Jatropha seeds) which was active against Aspergillus niger and Tamarindus indica (Tamarind) which showed antimicrobial activity against staphylococcus and Escherichia coli The most common major constituents of the fixed oils: were 14-methyl pendecanoic acid methyl ester 13.71%, 11-octadecanoic acid methyl ester 23.68%, Heptacosanoic acid methyl ester 10.22% and 9, 12-octadecadienoic acid methyl ester 17.44% for the Tamarindus indica While for Jatropha seeds oil 14- methylpentadecenoic acid methyl ester 12.31%, Hexadecanoic acid methyl ester 7.75%, 11- octadecanoic acid methyl ester 43.32% and 9- octadecenoic acid methyl ester 21.98% were obtained. Minimum inhibition concentrations of 12.50mg/ml were recorded for all test organisms by Jatropha seeds oil except for Escherichia coli which showed 6.25mg/ml. Both Tamarindus indica and Jatropha curcas showed minimum bactericidal activities at 12.50mg/ml were evaluated for all the samples. Recommendations for further research were also proffered.

scholarly journals Phytochemical and antibacterial properties of sea cucumber (Muelleria lecanora) from Barrang Lompo Islands, Makassar South Sulawesi

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1885-1895
Author(s):  
M. Yusuf ◽  
Nur Fitriani U.A. ◽  
L. Mahyati ◽  
M. Imran
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Sea Cucumber ◽  
Antibacterial Properties ◽  
Acid Methyl Ester ◽  
Diffusion Method ◽  
Acid Methyl ◽  
Pharmaceutical Industries

Barrang Lompo Island Waters is home for different species of marine biota of sea cucumber (Muelleria lecanora). Many sea cucumber species have been used as health supplements because they contain bioactive compounds that are beneficial to people in Indonesia. Given this, our study was designed to investigate the phytochemical, and antibacterial properties of crude acetone, methanol and hexane extract of sea cucumber using maceration extraction methods. The sea cucumber extract was prepared and the phytochemical profile was studied by analysing Gas Chromatography-Mass Spectrometry (GC-MS). Results showed that the extracts were a complex mixture of numerous compounds; many of which were present in trace amounts antioxidants and antimicrobial; hexadecanoic acid, methyl ester, 9-octadecenoic acid (z) -, methyl ester (stearic acid methyl ester), octadecanoic acid, methyl ester, 2-[(hexadecyloxy)methyl]oxirane, cholest5-en-3-yl acetate, ergosta-14,22-dien-3-ol, acetate,(3.beta.,5.alpha.,22e), 5,8,11,14- eicosatetraenoic acid, methyl ester, (all-z) epa/omega 3, pentacosane, hexatriacontane, and 9-hexadecenoic acid, methyl ester, (Z). The extract was also evaluated for activity against three pathogenic bacterial strains (Escherichia coli, Staphylococcus aureus and Salmonella) using the disc diffusion method. The extract exhibited clear zones of inhibition against the tested bacteria. Maximum inhibitory zone concentration values were demonstrated to be: Escherichia coli = 6.84 mm , Staphylococcus aureus = 7.22 mm, and Salmonella = 7.87 mm. These results revealed the significant potential of sea cucumber as a source of antioxidants and antimicrobial agents and also highlight the necessity of further purification and characterisation of solitary bioactive compounds for their prospective applications in pharmaceutical industries, food, and nutraceutical (food functional)

scholarly journals IDENTIFIKASI DAN UJI AKTIVITAS ANTIBAKTERI FRAKSI TERAKTIF DAUN PACAR KUKU (Lawsonia inermis Linn.) TERHADAP Staphylococcus aureus DAN Escherischia coli

Molekul ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. 178
Author(s):  
Nestri Handayani ◽  
Aprilia Fitriana ◽  
Desi Suci Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Acid Methyl Ester ◽  
Octadecanoic Acid ◽  
Hexadecanoic Acid ◽  
Lawsonia Inermis ◽  
Acid Methyl

Telah dilakukan uji aktivitas antibakteri fraksi teraktif daun pacar kuku (Lawsonia inermis Linn.) terhadap bakteriStaphylococcus aureusdan  Escherichia coli. Fraksi teraktif diperoleh dari hasil pemisahan kromatografi vacum cair (KVC) ekstrak etanol daun pacar kuku (Lawsonia inermis Linn.) yang telah melalui tahap maserasi dengan pelarut etanol 70%. Proses KVC dilakukan dengan  menggunakan tiga eluen yaitu n-heksan, etil asetat, dan etanol. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar. Komponen kimia dalam fraksi teraktif diidentifikasi melalui skrining fitokimia dengan metode kromatografi lapis tipis (KLT) dan kromatografi gas-spektrometer massa (GC-MS).Uji aktivitas antibakteri menunjukkan bahwa ekstrak etanol dan fraksi-fraksi daun pacar kuku (Lawsonia inermis Linn.) mampu menghambat pertumbuhan bakteri Staphylococcus aureus danEscherichia coli. Fraksi etil asetat adalah fraksi teraktif anti bakteri.Identifikasi senyawa dalam fraksi teraktif melalui kromatografi lapis tipis (KLT) menunjukkan adanya golongan senyawa flavonoid, terpenoid, antrakuinon, saponin, dan asam lemak. Analisis GC-MS menunjukkan adanya senyawa dominan 2-pentanone,4-hydroxy-4 methy, coumarin, hexadecanoic acid, methyl ester, dan 10-octadecanoic acid.

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