scholarly journals Study of mechanisms of electric field-induced DNA transfection. II. Transfection by low-amplitude, low-frequency alternating electric fields

1990 ◽  
Vol 58 (4) ◽  
pp. 897-903 ◽  
Author(s):  
T.D. Xie ◽  
T.Y. Tsong
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Low Frequency ◽  
Dna Transfection ◽  
Alternating Electric Fields ◽  
Low Amplitude

scholarly journals Low frequency weak electric fields can induce structural changes in water

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260967
Author(s):  
Iman Rad ◽  
Rainer Stahlberg ◽  
Kurt Kung ◽  
Gerald H. Pollack
Keyword(s):  
Contact Angle ◽  
Electric Field ◽  
Electric Fields ◽  
Structural Changes ◽  
Low Frequency ◽  
Bulk Water ◽  
Platinum Electrodes ◽  
Exclusion Zone ◽  
Alternating Electric Fields ◽  
E 600

Low frequency electric fields were exposed to various water samples using platinum electrodes mounted near the water surface. Responses were monitored using a spectro-radiometer and a contact-angle goniometer. Treatment of DI (deionized), EZ (Exclusion Zone), and bulk water with certain electromagnetic frequencies resulted in a drop of radiance persisting for at least half an hour. Compared to DI water, however, samples of EZ and bulk water showed lesser radiance drop. Contact-angle goniometric results confirmed that when treated with alternating electric fields (E = 600 ± 150 V/m, f = 7.8 and 1000 Hz), droplets of EZ and bulk water acquired different charges. The applied electric field interacted with EZ water only when electrodes were installed above the chamber, but not beneath. Further, when DI water interacted with an electric field applied from above (E = 600 ± 150 V/m, f = 75 Hz), its radiance profile became similar to that of EZ water. Putting these last two findings together, one can say that application of an electric field on DI water from above (E = 600 ± 150 V/m, f = 7.8 to 75 Hz) may induce a molecular ordering in DI water similar to that of EZ water.

Pulsed DC electric fields couple to natural NAD(P)H oscillations in HT-1080 fibrosarcoma cells

2001 ◽  
Vol 114 (8) ◽  
pp. 1515-1520 ◽  
Author(s):  
A.J. Rosenspire ◽  
A.L. Kindzelskii ◽  
H.R. Petty
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Cell Function ◽  
Low Frequency ◽  
Reactive Oxygen Metabolites ◽  
Pulsed Dc ◽  
Fibrosarcoma Cells ◽  
Chemotactic Factors ◽  
Oxygen Metabolites ◽  
Dc Electric Fields

Previously, we have demonstrated that NAD(P)H levels in neutrophils and macrophages are oscillatory. We have also found that weak ultra low frequency AC or pulsed DC electric fields can resonate with, and increase the amplitude of, NAD(P)H oscillations in these cells. For these cells, increased NAD(P)H amplitudes directly signal changes in behavior in the absence of cytokines or chemotactic factors. Here, we have studied the effect of pulsed DC electric fields on HT-1080 fibrosarcoma cells. As in neutrophils and macrophages, NAD(P)H levels oscillate. We find that weak (~10(-)(5) V/m), but properly phased DC (pulsed) electric fields, resonate with NAD(P)H oscillations in polarized and migratory, but not spherical, HT-1080 cells. In this instance, electric field resonance signals an increase in HT-1080 pericellular proteolytic activity. Electric field resonance also triggers an immediate increase in the production of reactive oxygen metabolites. Under resonance conditions, we find evidence of DNA damage in HT-1080 cells in as little as 5 minutes. Thus the ability of external electric fields to effect cell function and physiology by acting on NAD(P)H oscillations is not restricted to cells of the hematopoietic lineage, but may be a universal property of many, if not all polarized and migratory eukaryotic cells.

scholarly journals BEM Modelling of High Voltage ELF electric field applied to a 3D pregnant woman model

2010 ◽  
Vol 6 (1) ◽  
pp. 31 ◽  
Author(s):  
Cristina Peratta ◽  
Andres Peratta ◽  
Dragan Poljak
Keyword(s):  
Pregnant Woman ◽  
Electric Field ◽  
Electric Fields ◽  
High Voltage ◽  
Medical Information ◽  
Three Dimensional ◽  
Low Frequency ◽  
Element Model ◽  
The Body ◽  
Geometrical Properties

The paper introduces a three dimensional multidomainboundary element model of a pregnant woman and foetus for the analysis of exposure to high voltage extremely low frequency electric fields. The definition of the differentphysical and geometrical properties of the relevant tissues is established according to medical information available in existing literature. The model takes into account changes in geometry, body mass, body fat, and overall chemical composition in the body which influence the electrical properties, throughout the different gestational periods. The developed model is used to solve the case of exposure to overhead power transmission lines at different stages of pregnancy including weeks 8, 13, 26 and 38. The results obtained are in line with those published in the earlier works considering different approaches. In addition, a sensitivity analysis involving varying scenarios of conductivity, foetus postures and geometry for each stage is defined and solved. Finally, a correlation between the externally applied electric field and the current density inside the foetus is established and the zones of maximum exposure are identified.

Manipulation and assembly behavior of Spirulina-templated microcoils in the electric field

RSC Advances ◽  
2016 ◽  
Vol 6 (80) ◽  
pp. 76716-76723 ◽  
Author(s):  
Xinghao Li ◽  
Jun Cai ◽  
Lili Sun ◽  
Yue Yue ◽  
Deyuan Zhang
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Alternating Electric Fields ◽  
Assembly Behavior

Manipulation and assembly of complicated metallic Spirulina-templated microcoils can be achieved through alternating electric fields.

Sensitivity to change in electrical environment: a new bioelectric effect

1980 ◽  
Vol 239 (5) ◽  
pp. R424-R427 ◽  
Author(s):  
A. A. Marino ◽  
J. M. Cullen ◽  
M. Reichmanis ◽  
R. O. Becker ◽  
F. X. Hart
Keyword(s):  
Electric Field ◽  
Blood Cell ◽  
Electric Fields ◽  
Red Blood Cell ◽  
Low Frequency ◽  
Sensitivity To Change ◽  
Bioelectric Effect ◽  
Cell Concentrations ◽  
Inappropriate Choice

The action of a 60-Hz, 5 kV/m electric field on erythrocyte parameters in mice was determined. No effects attributable to the magnitude of the field were found, but a transition either from or to an environment containing the field caused decreased red blood cell concentrations and decreased hematocrits. The failure of others to observe effects on erythrocyte parameters following exposure to low-frequency electric fields may have been due to an inappropriate choice of duration of exposure.

scholarly journals ELECTROPHORESIS IN THE TOTAL (CONSTANT AND ALTERNATING) ELECTRIC FIELD. II. PECULIARITIES OF THE COMBINED IMPACT OF ALTERNATING AND CONSTANT ELECTRIC FIELDS

2020 ◽  
Vol 30 (4) ◽  
pp. 46-51
Author(s):  
B. P. Sharfarets ◽  
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Constant Electric Field ◽  
Electrokinetic Phenomena ◽  
Alternating Electric Fields ◽  
Alternating Electric Field ◽  
Electroacoustic Transducer ◽  
Dispersed Medium ◽  
Hydrodynamic Field ◽  
Combined Impact

The hydrodynamics of electrophoresis under the simultaneous impact of constant and alternating electric fields is considered. It has been shown that when the constant and alternating external fields are combined, the energy of the constant electric field is transferred into the alternating hydrodynamic field. An example is given of a dispersed medium in which a giant dispersion of the dielectric constant can arise, which in turn can contribute to an increase in the total electrophoresis rate. Analogies of the behavior of the considered dispersed medium with the action of an electroacoustic transducer based on the use of electrokinetic phenomena are given.

Characterization of interactive force acting on colloidal particles near an electrode in presence of a high-frequency (>10 kHz) AC electric field using particle diffusometry

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Kshitiz Gupta ◽  
Dong Hoon Lee ◽  
Steven T. Wereley ◽  
Stuart J. Williams
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Particle Motion ◽  
Electrode Surface ◽  
High Frequency ◽  
Colloidal Particles ◽  
Low Frequency ◽  
Double Layers ◽  
Average Height ◽  
Ac Electric Field

Colloidal particles like polystyrene beads and metallic micro and nanoparticles are known to assemble in crystal-like structures near an electrode surface under both DC and AC electric fields. Various studies have shown that this self-assembly is governed by a balance between an attractive electrohydrodynamic (EHD) force and an induced dipole-dipole repulsion (Trau et al., 1997). The EHD force originates from electrolyte flow caused by interaction between the electric field and the polarized double layers of both the particles and the electrode surface. The particles are found to either aggregate or repel from each other on application of electric field depending on the mobility of the ions in the electrolyte (Woehl et al., 2014). The particle motion in the electrode plane is studied well under various conditions however, not as many references are available in the literature that discuss the effects of the AC electric field on their out-of-plane motion, especially at high frequencies (>10 kHz). Haughey and Earnshaw (1998), and Fagan et al. (2005) have studied the particle motion perpendicular to the electrode plane and their average height from the electrode mostly in presence of DC or low frequency AC (<1 kHz) electric field. However, these studies do not provide enough insight towards the effects of high frequency (>10 kHz) electric field on the particles’ motion perpendicular to the electrode plane.  

Choice of Electrode Material for Detecting Low Frequency Electric Field in Sea Water

2011 ◽  
Vol 239-242 ◽  
pp. 137-140 ◽  
Author(s):  
Yu Su Song ◽  
Kun Zhang ◽  
Pan Zuo
Keyword(s):  
Solid State ◽  
Electric Field ◽  
Electric Fields ◽  
Electrode Material ◽  
Sea Water ◽  
Low Frequency ◽  
Low Frequencies ◽  
Voltage Difference ◽  
Frequency Electric Field ◽  
Sensitive Electrode

Seven types of electrodes have been selected. DC resistances, AC impedances and voltage difference of the electrode pairs have been studied, according to the requirement of low and extra low frequencies electric fields detection in sea water. The feasibilities of these electrodes used for detecting these signals have been studied and explained on theories. The results show that the all-solid-state Ag/AgCl electrode is the most sensitive electrode with its very low DC resistances, AC impedances and the voltage difference controlled within 20μV, which makes it detecting low frequency electric field accurately.

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