149 Resveratrol Sensitizes Colorectal Cancer Cells to Oxaliplatin-Induced Cell Death - Possible Key Role of Inhibitor of Apoptosis Proteins

The transient receptor potential vanilloid member 4 (TRPV4) is a non-selective calcium (Ca2+)-permeable channel which is widely expressed in different types of tissues including the lungs, liver, kidneys and salivary gland. TRPV4 has been shown to serve as a cellular sensor where it is involved in processes such as osmoregulation, cell volume regulation and thermoregulation. Emerging evidence suggests that TRPV4 also plays important roles in several aspects of cancer progression. Despite the reported roles of TRPV4 in several forms of cancers, the role of TRPV4 in human colorectal cancer remains largely unexplored. In the present study, we sought to establish the potential role of TRPV4 in colorectal cancer by assessing TRPV4 expression levels and investigating whether TRPV4 pharmacological modulation may alter cell proliferation, cell cycle and cell death in colorectal cancer cells. Quantitative real-time PCR analysis revealed that TRPV4 mRNA levels were significantly lower in HT-29 cells than normal colon CCD-18Co cells. However, TRPV4 mRNA was absent in HCT-116 cells. Pharmacological activation of TRPV4 with GSK1016790A significantly enhanced the proliferation of HT-29 cells while TRPV4 inhibition using RN 1734 decreased their proliferation. Increased proliferation in GSK1016790A-treated HT-29 cells was attenuated by co-treatment with RN 1734. Pharmacological modulation of TRPV4 had no effect on the cell cycle progression but promoted cell death in HT-29 cells. Taken together, these findings suggest differential TRPV4 expression levels in human colorectal cancer cells and that pharmacological modulation of TRPV4 produces distinct effects on the proliferation and induces cell death in HT-29 cells.

Download Full-text

A novel cell type-specific role of p38α in the control of autophagy and cell death in colorectal cancer cells

Cell Death and Differentiation ◽

10.1038/sj.cdd.4402076 ◽

2006 ◽

Vol 14 (4) ◽

pp. 693-702 ◽

Cited By ~ 88

Author(s):

F Comes ◽

A Matrone ◽

P Lastella ◽

B Nico ◽

F C Susca ◽

...

Keyword(s):

Colorectal Cancer ◽

Cell Death ◽

Cancer Cells ◽

Specific Role ◽

Cell Type ◽

Colorectal Cancer Cells ◽

Cell Type Specific

Download Full-text

Mechanisms of cisplatin-induced cell death in malignant mesothelioma cells: Role of inhibitor of apoptosis proteins (IAPs) and caspases

International Journal of Oncology ◽

10.3892/ijo.2012.1715 ◽

2012 ◽

Vol 42 (2) ◽

pp. 444-452 ◽

Cited By ~ 12

Author(s):

INEZ L. CREGAN ◽

ARUN M. DHARMARAJAN ◽

SIMON A. FOX

Keyword(s):

Cell Death ◽

Malignant Mesothelioma ◽

Inhibitor Of Apoptosis ◽

Inhibitor Of Apoptosis Proteins ◽

Apoptosis Proteins

Download Full-text

Role of nonsteroidal anti-inflammatory drug-activated gene-1 in docetaxel-induced cell death of human colorectal cancer cells with different p53 status

Archives of Pharmacal Research ◽

10.1007/s12272-011-0219-8 ◽

2011 ◽

Vol 34 (2) ◽

pp. 323-330 ◽

Cited By ~ 9

Author(s):

Il-Yeob Kim ◽

Su-Young Park ◽

Youra Kang ◽

Dinesh Thapa ◽

Han Gon Choi ◽

...

Keyword(s):

Colorectal Cancer ◽

Cell Death ◽

Cancer Cells ◽

Human Colorectal Cancer ◽

Inflammatory Drug ◽

Colorectal Cancer Cells ◽

P53 Status ◽

Anti Inflammatory ◽

Human Colorectal Cancer Cells

Download Full-text

Faculty Opinions recommendation of HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1003672.38258 ◽

2002 ◽

Author(s):

John Abrams

Keyword(s):

Cell Death ◽

Serine Protease ◽

Protease Activity ◽

Inhibitor Of Apoptosis ◽

Inhibitor Of Apoptosis Proteins ◽

Serine Protease Activity ◽

Apoptosis Proteins

Download Full-text

The essential role of TAp73 in bortezomib-induced apoptosis in p53-deficient colorectal cancer cells

Scientific Reports ◽

10.1038/s41598-017-05813-z ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 19

Author(s):

Yasamin Dabiri ◽

Sara Kalman ◽

Clara-Marie Gürth ◽

Jee Young Kim ◽

Viola Mayer ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Essential Role ◽

Colorectal Cancer Cells ◽

Induced Apoptosis

Download Full-text

Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells

BioMed Research International ◽

10.1155/2020/5053975 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Wanjuan Xue ◽

Yongcheng Liu ◽

Ningning Xin ◽

Jiyu Miao ◽

Juan Du ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

Human Colon ◽

Caspase 9 ◽

Human Colon Cancer ◽

Expression Levels ◽

Colorectal Cancer Cells ◽

Human Colorectal Cancer Cells

The study is aimed at investigating the role of Nei endonuclease VIII-like1 (NEIL1) in the pathogenesis of colorectal cancer (CRC). The human CRC (HCT116 and SW480) cells were subjected to the siRNA silencing and recombinant plasmid overexpression of NEIL1. Transfection of siNEIL1 significantly inhibited the cell growth. It also increased the Bax expression levels, while it decreased the Bcl-2 expression levels in human CRC cells, leading the Bax/Bcl-2 balance toward apoptosis. Moreover, the apoptosis was promoted through the caspase-9 signaling pathway. One the other hand, high expression of NEIL1 promoted the cell viability and reduced the apoptosis, inducing the balance of Bax/Bcl-2 in the human colon cancer cells to be antiapoptotic. In addition, the caspase-9 signaling pathway inhibited apoptosis, contrary to the results obtained by downregulating NEIL1 expression. Furthermore, NEIL1 was negatively regulated by miR-7-5p, indicating that miR-7-5p inhibited the NEIL1 expression after transcription. Overexpression of miR-7-5p reversed the effects of NEIL1 on these CRC cells. In conclusion, NEIL1 promotes the proliferation of CRC cells, which is regulated negatively by miR-7-5p. These findings suggest that NEIL1 is a potential therapeutic target for CRC.

Download Full-text

p53 Enhances Artemisia annua L. Polyphenols-Induced Cell Death Through Upregulation of p53-Dependent Targets and Cleavage of PARP1 and Lamin A/C in HCT116 Colorectal Cancer Cells

International Journal of Molecular Sciences ◽

10.3390/ijms21239315 ◽

2020 ◽

Vol 21 (23) ◽

pp. 9315

Author(s):

Eun Joo Jung ◽

Won Sup Lee ◽

Anjugam Paramanantham ◽

Hye Jung Kim ◽

Sung Chul Shin ◽

...

Keyword(s):

Colorectal Cancer ◽

Cell Death ◽

Cancer Cells ◽

Artemisia Annua ◽

Flow Cytometric Analysis ◽

Lamin A ◽

Artemisia Annua L ◽

Anticancer Effects ◽

Colorectal Cancer Cells ◽

Hct116 Cells

Plant-derived natural polyphenols exhibit anticancer activity without showing any noticeable toxicities to normal cells. The aim of this study was to investigate the role of p53 on the anticancer effect of polyphenols isolated from Korean Artemisia annua L. (pKAL) in HCT116 human colorectal cancer cells. We confirmed that pKAL induced reactive oxygen species (ROS) production, propidium iodide (PI) uptake, nuclear structure change, and acidic vesicles in a p53-independent manner in p53-null HCT116 cells through fluorescence microscopy analysis of DCF/PI-, DAPI-, and AO-stained cells. The pKAL-induced anticancer effects were found to be significantly higher in p53-wild HCT116 cells than in p53-null by hematoxylin staining, CCK-8 assay, Western blot, and flow cytometric analysis of annexin V/PI-stained cells. In addition, expression of ectopic p53 in p53-null cells was upregulated by pKAL in both the nucleus and cytoplasm, increasing pKAL-induced cell death. Moreover, Western bot analysis revealed that pKAL-induced cell death was associated with upregulation of p53-dependent targets such as p21, Bax and DR5 and cleavage of PARP1 and lamin A/C in p53-wild HCT116 cells, but not in p53-null. Taken together, these results indicate that p53 plays an important role in enhancing the anticancer effects of pKAL by upregulating p53 downstream targets and inducing intracellular cell death processes.

Download Full-text