Ngal in Pancreatic Juice Helps to Discriminate Chronic Pancreatitis and Pancreatic Cancer Patients From Healthy Controls

Several exosome proteins, miRNAs and KRAS mutations have been investigated in the hope of carrying out the early detection of pancreatic cancer with high sensitivity and specificity, but they have proven to be insufficient. Exosome RNAs, however, have not been extensively evaluated in the diagnosis of pancreatic cancer. The purpose of this study was to investigate the potential of circulating exosome RNAs in pancreatic cancer detection. By retrieving RNA-seq data from publicly accessed databases, differential expression and random-effects meta-analyses were performed. The results showed that pancreatic cancer had a distinct circulating exosome RNA signature in healthy individuals, and that the top 10 candidate exosome RNAs could distinguish patients from healthy individuals with an area under the curve (AUC) of 1.0. Three (HIST2H2AA3, LUZP6 and HLA-DRA) of the 10 genes in exosomes had similar differential patterns to those in tumor tissues based on RNA-seq data. In the validation dataset, the levels of these three genes in exosomes displayed good performance in distinguishing cancer from both chronic pancreatitis (AUC = 0.815) and healthy controls (AUC = 0.8558), whereas a slight difference existed between chronic pancreatitis and healthy controls (AUC = 0.586). Of the three genes, the level of HIST2H2AA3 was positively associated with KRAS status. However, there was no significant difference in the levels of the three genes across the disease stages (stages I–IV). These findings indicate that circulating exosome RNAs have a potential early detection value in pancreatic cancer, and that a distinct exosome RNA signature exists in distinguishing pancreatic cancer from healthy individuals.

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Deletions of p16/MTS-1 gene is a frequent event in pancreatic juice from patients with pancreatic cancer and chronic pancreatitis

Gastroenterology ◽

10.1016/s0016-5085(98)82308-1 ◽

1998 ◽

Vol 114 ◽

pp. A567

Author(s):

M. Bouisson ◽

P. Pagès ◽

L. Buscail ◽

O. Croizet ◽

C. Bureau ◽

...

Keyword(s):

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Pancreatic Juice ◽

Frequent Event

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The Risk Factors of Pancreatic Cancer Patients in Dr. Cipto Mangunkusumo National Hospital, Jakarta During 2014-2019

The Indonesian Journal of Gastroenterology Hepatology and Digestive Endoscopy ◽

10.24871/2122020120-125 ◽

2020 ◽

Vol 21 (2) ◽

pp. 120-125

Author(s):

Kaka Renaldi ◽

Teddy Septianto ◽

Dadang Makmun

Keyword(s):

Diabetes Mellitus ◽

Risk Factors ◽

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Family History ◽

Alcohol Consumption ◽

Cancer Patients ◽

Obesity And Diabetes ◽

History Of ◽

Age Adjusted Rates

Background: Pancreatic cancer is a very rare cancer with age-adjusted rates ranging from about 5 to 10 new cases per 100,000 persons per year. It has one of the worst prognoses of any type of cancer, with a 5-year survival rate of only 4.6%. Several risk factors have been identified, including older age, smoking, familial history of pancreatic cancer, obesity, chronic pancreatitis, diabetes mellitus, and alcohol consumption.Method: This was a descriptive study describing the risk factors of patients who were diagnosed with pancreatic cancer in the period between 1 January 2014 – 1 January 2019 at the Cipto Mangunkusumo National Referral Hospital (RSCM) Jakarta. Data were obtained from the medical records and Endoscopic Retrograde Cholangiopancreatography (ERCP) database from the RSCM Gastrointestinal Endoscopy Center.Results: From January 2014 to January 2019 there were 123 patients with newly diagnosed pancreatic cancer in RSCM. The mean age was 52 years old. The incidence of pancreatic cancer is more common in men (53%) than women (47%). The most common risk factor identified is smoking which was found in 29% of patients, followed by obesity at 27.9% and a history of diabetes mellitus at 19.5%. Risk factors with a fairly low prevalence include alcohol consumption at 9.7% and chronic pancreatitis at 2.4%. No family history of pancreatic cancer is identified in any subject.Conclusion: Smoking, obesity, and diabetes mellitus are common risk factors in pancreatic cancer patients. In contrast, chronic pancreatitis, alcohol consumption, and family history of pancreatic cancer are less commonly identified in patients.

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