The Abundance of Clostridium Hathewayi, a Potent Inducer of T Helper 17 (Th17) Cells, is Associated with the Disease Severity of Crohn's Disease

2017 ◽  
Vol 152 (5) ◽  
pp. S993 ◽  
Author(s):  
Kyohei Nishino ◽  
Hirotsugu Imaeda ◽  
Shigeki Sakai ◽  
Masashi Ohno ◽  
Atsushi Nishida ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Disease Severity ◽  
Th17 Cells ◽  
T Helper ◽  
T Helper 17 ◽  
Potent Inducer

Ustekinumab Improves Active Crohn’s Disease by Suppressing the T Helper 17 Pathway

Digestion ◽  
2021 ◽  
pp. 1-10
Author(s):  
Yutaro Ihara ◽  
Takehiro Torisu ◽  
Kohta Miyawaki ◽  
Junji Umeno ◽  
Keisuke Kawasaki ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Cell Differentiation ◽  
T Cell ◽  
Crohn's Disease ◽  
Peripheral Blood ◽  
Colonic Mucosa ◽  
Enrichment Analysis ◽  
T Cell Subsets ◽  
T Helper ◽  
T Helper 17

<b><i>Background:</i></b> Ustekinumab (UST), an antibody targeting the p40 subunit of interleukin (IL)-12 and IL-23, is effective in treating Crohn’s disease (CD). To clarify the mechanism of UST, we investigated T-cell differentiation in CD patients treated with UST. <b><i>Methods:</i></b> Twenty-seven patients with active CD were enrolled in this study. Seventeen patients were treated with UST, and 10 patients were treated with anti-tumor necrosis factor (TNF)-alpha therapy. The changes in the proportions of T-cell subsets after these therapies were analyzed by flow cytometry. Comprehensive gene expression changes in the colonic mucosa were also evaluated. <b><i>Results:</i></b> The frequency of T helper (Th) 17 cells was significantly decreased in the peripheral blood of patients with active CD after UST therapy. Anti-TNF therapy had a minimal effect on Th17 cells but increased the proportion of regulatory T cells. Enrichment analysis showed the expression of genes involved in the Th17 differentiation pathway was downregulated in the colonic mucosa after UST but not anti-TNF therapy. There were no common differentially expressed genes between CD patients treated with UST and anti-TNF therapy, suggesting a clear difference in their mechanism of action. <b><i>Conclusion:</i></b> In patients with active CD, UST therapy suppressed Th17 cell differentiation both in the peripheral blood and colonic tissues.

scholarly journals Commensal-Specific CD4+ Cells From Patients With Crohn’s Disease Have a T-Helper 17 Inflammatory Profile

2016 ◽  
Vol 151 (3) ◽  
pp. 489-500.e3 ◽  
Author(s):  
Elisabeth Calderón-Gómez ◽  
Helena Bassolas-Molina ◽  
Rut Mora-Buch ◽  
Isabella Dotti ◽  
Núria Planell ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
T Helper ◽  
Cd4 Cells ◽  
T Helper 17 ◽  
Inflammatory Profile

scholarly journals Effects of Anti-TNFαTreatment on Mucosal Expression of IL-17A, IL-21, and IL-22 and Cytokine-Producing T Cell Subsets in Crohn’s Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Anders Dige ◽  
Maria K. Magnusson ◽  
Claus Uhrenholt ◽  
Tue Kruse Rasmussen ◽  
Tue Kragstrup ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Crohn’S Disease ◽  
T Cell ◽  
Crohn's Disease ◽  
Th17 Cells ◽  
T Cell Subsets ◽  
T Helper 17 ◽  
Early Effect ◽  
Before And After

T helper 17 (Th17) cells produce interleukin (IL) 17-A. In addition, Th17 cells produce IL-21 and IL-22. Th17 cells have a disease-promoting role in Crohn’s disease (CD). We investigated the effects of anti-TNFαtreatment on mucosal gene expression (qPCR) of IL-17A, IL-21, and IL-22 as well as on the frequency of lamina propria (LP) T cell subsets producing these cytokines (flow cytometry) in 12 active CD patients before and after 4 weeks of anti-TNFαtreatment with adalimumab. At baseline, in inflamed mucosa we found increased gene expression of IL-17A and IL-22 but not IL-21 when compared to noninflamed mucosa. There were increased frequencies of IL-21-producing LP T cells but no differences in the frequencies of IL-17A- or IL-22-producing LP T cells when comparing inflamed versus noninflamed mucosa at baseline. There were no changes in the mucosal gene expression of IL-17A, IL-21, and IL-22 or the frequencies of IL-17A-, IL-21- and IL-22-producing LP T cell subsets between baseline and following 4 weeks of adalimumab initiation. Our results do not support the hypothesis that anti-TNFαtreatment has an early effect on the mucosal levels of IL-17A, IL-21, and IL-22 or LP T cell production of these cytokines in CD.

scholarly journals Pivotal Roles of T-Helper 17-Related Cytokines, IL-17, IL-22, and IL-23, in Inflammatory Diseases

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Ning Qu ◽  
Mingli Xu ◽  
Izuru Mizoguchi ◽  
Jun-ichi Furusawa ◽  
Kotaro Kaneko ◽  
...  
Keyword(s):  
Th17 Cell ◽  
Th17 Cells ◽  
Functional Role ◽  
Inflammatory Diseases ◽  
Interleukin 17 ◽  
T Helper ◽  
T Helper 17 ◽  
Autoimmune Encephalomyelitis ◽  
Experimental Autoimmune

T-helper 17 (Th17) cells are characterized by producing interleukin-17 (IL-17, also called IL-17A), IL-17F, IL-21, and IL-22 and potentially TNF-α and IL-6 upon certain stimulation. IL-23, which promotes Th17 cell development, as well as IL-17 and IL-22 produced by the Th17 cells plays essential roles in various inflammatory diseases, such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, colitis, and Concanavalin A-induced hepatitis. In this review, we summarize the characteristics of the functional role of Th17 cells, with particular focus on the Th17 cell-related cytokines such as IL-17, IL-22, and IL-23, in mouse models and human inflammatory diseases.

Response to treatment is more important than disease severity at diagnosis for prediction of early relapse in new-onset paediatric Crohn’s disease

2018 ◽  
Vol 48 (11-12) ◽  
pp. 1242-1250 ◽  
Author(s):  
Tomer Ziv-Baran ◽  
Séamus Hussey ◽  
Malgorzata Sladek ◽  
Jorge Amil Dias ◽  
Javier Martin de Carpi ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Disease Severity ◽  
Response To Treatment ◽  
Early Relapse ◽  
Paediatric Crohn’S Disease ◽  
New Onset

scholarly journals The protective effect of curcumin on rats with DSS-induced ulcerative colitis and its mechanisms

2021 ◽  
Author(s):  
Jing Guo ◽  
Yan-yan Zhang ◽  
Mei Sun ◽  
Ling-fen Xu
Keyword(s):  
Ulcerative Colitis ◽  
Th17 Cells ◽  
Dextran Sulfate ◽  
Activity Index ◽  
Disease Activity Index ◽  
Treg Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
T Helper ◽  
T Helper 17 ◽  
Inflammatory Bowel

Abstract Aim This study aimed to explore effect of curcumin on inflammatory bowel disease (IBD) in rats and its mechanism.Methods: A dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) rat model was established. The disease activity index (DAI) scores were calculated. The histopathological damage scores were determined by haematoxylin-eosin (H&E) staining. Regulatory T (Treg) cells and T helper 17 (Th17) cells in the spleen were analysed by flow cytometry. The levels of interleukin (IL)-10 and IL-17A were determined by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the DSS model group, the curcumin group exhibited significantly reduced DAI scores and improvements in histopathological damage. The expression of CD4+IL-17+ Th17 cells was significantly lower and the expression of CD4+CD25+Foxp3+ Treg cells was significantly higher in the curcumin group than in the DSS group.Conclusion: Curcumin may be a new and effective treatment for IBD by regulating the balance of Treg/Th17 cells and the expression of IL-10 and IL-17A.

TNF Promoter Polymorphisms and Modulation of Growth Retardation and Disease Severity in Pediatric Crohn's Disease

2005 ◽  
Vol 100 (7) ◽  
pp. 1598-1604 ◽  
Author(s):  
Arie Levine ◽  
Raanan Shamir ◽  
Eytan Wine ◽  
Batya Weiss ◽  
Amir Karban ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Disease Severity ◽  
Growth Retardation ◽  
Promoter Polymorphisms
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