I209 Effects of progesterone on apoptsis in the human extravillous trophoblasts

2009 ◽  
Vol 107 ◽  
pp. S52-S52
Author(s):  
T. Maruo ◽  
J. Liu ◽  
H. Matsuo
Keyword(s):  
Extravillous Trophoblasts

scholarly journals HMGA1 Is a Potential Driver of Preeclampsia Pathogenesis by Interference with Extravillous Trophoblasts Invasion

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 822
Author(s):  
Keiichi Matsubara ◽  
Yuko Matsubara ◽  
Yuka Uchikura ◽  
Katsuko Takagi ◽  
Akiko Yano ◽  
...  
Keyword(s):  
Protein A ◽  
High Mobility ◽  
Extravillous Trophoblast ◽  
High Mobility Group Protein ◽  
Successful Pregnancy ◽  
Group Protein ◽  
Serious Disease ◽  
Proliferation And Invasion ◽  
Extravillous Trophoblasts ◽  
Fertilized Egg

Preeclampsia (PE) is a serious disease that can be fatal for the mother and fetus. The two-stage theory has been proposed as its cause, with the first stage comprising poor placentation associated with the failure of fertilized egg implantation. Successful implantation and placentation require maternal immunotolerance of the fertilized egg as a semi-allograft and appropriate extravillous trophoblast (EVT) invasion of the decidua and myometrium. The disturbance of EVT invasion during implantation in PE results in impaired spiral artery remodeling. PE is thought to be caused by hypoxia during remodeling failure–derived poor placentation, which results in chronic inflammation. High-mobility group protein A (HMGA) is involved in the growth and invasion of cancer cells and likely in the growth and invasion of trophoblasts. Its mechanism of action is associated with immunotolerance. Thus, HMGA is thought to play a pivotal role in successful pregnancy, and its dysfunction may be related to the pathogenesis of PE. The evaluation of HMGA function and its changes in PE might confirm that it is a reliable biomarker of PE and provide prospects for PE treatment through the induction of EVT proliferation and invasion during the implantation.

scholarly journals Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiao-Hui Wang ◽  
Sha Xu ◽  
Xiang-Yu Zhou ◽  
Rui Zhao ◽  
Yan Lin ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Embryo Implantation ◽  
Underlying Mechanism ◽  
Recurrent Spontaneous Abortion ◽  
Extravillous Trophoblast ◽  
Trophoblast Invasion ◽  
Iron Sulfur ◽  
Succinate Dehydrogenase Complex ◽  
Extravillous Trophoblasts ◽  
Control Study

AbstractDysregulated extravillous trophoblast invasion and proliferation are known to increase the risk of recurrent spontaneous abortion (RSA); however, the underlying mechanism remains unclear. Herein, in our retrospective observational case-control study we show that villous samples from RSA patients, compared to healthy controls, display reduced succinate dehydrogenase complex iron sulfur subunit (SDHB) DNA methylation, elevated SDHB expression, and reduced succinate levels, indicating that low succinate levels correlate with RSA. Moreover, we find high succinate levels in early pregnant women are correlated with successful embryo implantation. SDHB promoter methylation recruited MBD1 and excluded c-Fos, inactivating SDHB expression and causing intracellular succinate accumulation which mimicked hypoxia in extravillous trophoblasts cell lines JEG3 and HTR8 via the PHD2-VHL-HIF-1α pathway; however, low succinate levels reversed this effect and increased the risk of abortion in mouse model. This study reveals that abnormal metabolite levels inhibit extravillous trophoblast function and highlights an approach for RSA intervention.

O1. Dramatic upregulation of HIF-1α in the endovascular and extravillous trophoblasts at the maternal-fetal interface in preeclamptic pregnancies

2011 ◽  
Vol 1 (3-4) ◽  
pp. 257
Author(s):  
JongYun Hwang ◽  
Xiujun Fan ◽  
Joyce F. Sung ◽  
Sabita Dhal ◽  
Nihar R. Nayak ◽  
...  
Keyword(s):  
Extravillous Trophoblasts ◽  
Maternal Fetal Interface

scholarly journals Impaired Wnt5a signaling in extravillous trophoblasts: Relevance to poor placentation in early gestation and subsequent preeclampsia

2018 ◽  
Vol 13 ◽  
pp. 225-234 ◽  
Author(s):  
Mari Ujita ◽  
Eiji Kondoh ◽  
Yoshitsugu Chigusa ◽  
Haruta Mogami ◽  
Kaoru Kawasaki ◽  
...  
Keyword(s):  
Early Gestation ◽  
Extravillous Trophoblasts

scholarly journals Epigenetic changes in preterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth

2018 ◽  
Vol 28 (1) ◽  
pp. 84-95 ◽  
Author(s):  
Sneha Mani ◽  
Jayashri Ghosh ◽  
Yemin Lan ◽  
Suneeta Senapati ◽  
Teri Ord ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Critical Role ◽  
Spontaneous Preterm Birth ◽  
Gene Methylation ◽  
Vitro Fertilization ◽  
Genome Wide ◽  
Common Etiology ◽  
Adamts Gene ◽  
Extravillous Trophoblasts

Abstract Preterm birth (PTB) affects approximately 1 in 10 pregnancies and contributes to approximately 50% of neonatal mortality. However, despite decades of research, little is understood about the etiology of PTB, likely due to the multifactorial nature of the disease. In this study, we examined preterm and term placentas, from unassisted conceptions and those conceived using in vitro fertilization (IVF). IVF increases the risk of PTB and causes epigenetic change in the placenta and fetus; therefore, we utilized these patients as a unique population with a potential common etiology. We investigated genome-wide DNA methylation in placentas from term IVF, preterm IVF, term control (unassisted conception) and preterm control pregnancies and discovered epigenetic dysregulation of multiple genes involved in cell migration, including members of the ADAMTS family, ADAMTS12 and ADAMTS16. These genes function in extracellular matrix regulation and tumor cell invasion, processes replicated by invasive trophoblasts (extravillous trophoblasts (EVTs)) during early placentation. Though expression was similar between term and preterm placentas, we found that both genes demonstrate high expression in first- and second-trimester placenta, specifically in EVTs and syncytiotrophoblasts. When we knocked down ADAMTS12 or ADAMTS16in vitro, there was poor EVT invasion and reduced matrix metalloproteinase activity, reinforcing their critical role in placentation. In conclusion, utilizing a population at high risk for PTB, we have identified a role for ADAMTS gene methylation in regulating early placentation and susceptibility to PTB.

scholarly journals The HLA-G cycle provides for both NK tolerance and immunity at the maternal–fetal interface

2015 ◽  
Vol 112 (43) ◽  
pp. 13312-13317 ◽  
Author(s):  
Tamara Tilburgs ◽  
J. Henry Evans ◽  
Ângela C. Crespo ◽  
Jack L. Strominger
Keyword(s):  
Natural Killer Cells ◽  
Confocal Microscopy ◽  
Virus Infection ◽  
Natural Killer ◽  
Stromal Cells ◽  
Synapse Formation ◽  
Initial Stage ◽  
Extravillous Trophoblasts ◽  
Maternal Fetal Interface ◽  
Decidual Natural Killer Cells

The interaction of noncytotoxic decidual natural killer cells (dNK) and extravillous trophoblasts (EVT) at the maternal–fetal interface was studied. Confocal microscopy revealed that many dNK interact with a single large EVT. Filamentous projections from EVT enriched in HLA-G were shown to contact dNK, and may represent the initial stage of synapse formation. As isolated, 2.5% of dNK contained surface HLA-G. However, surface HLA-G–negative dNK contained internalized HLA-G. Activation of dNK resulted in the disappearance of internalized HLA-G in parallel with restoration of cytotoxicity. Surface HLA-G was reacquired by incubation with EVT. This HLA-G cycle of trogocytosis, endocytosis, degradation, and finally reacquisition provides a transient and localized acquisition of new functional properties by dNK upon interaction with EVT. Interruption of the cycle by activation of dNK by cytokines and/or viral products serves to ensure the NK control of virus infection at the interface, and is illustrated here by the response of dNK to human cytomegalo virus (HCMV)-infected decidual stromal cells. Thus, the HLA-G cycle in dNK can provide both for NK tolerance and antiviral immunity.

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