Pharmacological studies on involvement of histamine H2 receptor in skin barrier function and skin condition in hairless mice and guinea pigs

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

Download Full-text

Clove attenuates UVB-induced photodamage and repairs skin barrier function in hairless mice

Food & Function ◽

10.1039/c8fo00843d ◽

2018 ◽

Vol 9 (9) ◽

pp. 4936-4947 ◽

Cited By ~ 4

Author(s):

Eunson Hwang ◽

Pei Lin ◽

Hien T. T. Ngo ◽

Tae-Hoo Yi

Keyword(s):

Barrier Function ◽

Food Industry ◽

Skin Barrier ◽

Skin Barrier Function ◽

Syzygium Aromaticum ◽

Hairless Mice

Syzygium aromaticum L., commonly named clove, is widely used in the food industry due to its antioxidant and antibacterial capabilities.

Download Full-text

Atopic Dermatitis as a Multifactorial Skin Disorder. Can the Analysis of Pathophysiological Targets Represent the Winning Therapeutic Strategy?

Pharmaceuticals ◽

10.3390/ph13110411 ◽

2020 ◽

Vol 13 (11) ◽

pp. 411

Author(s):

Irene Magnifico ◽

Giulio Petronio Petronio ◽

Noemi Venditti ◽

Marco Alfio Cutuli ◽

Laura Pietrangelo ◽

...

Keyword(s):

Drug Delivery ◽

Atopic Dermatitis ◽

Barrier Function ◽

Scientific Evidence ◽

Alternative Therapy ◽

Skin Barrier ◽

Skin Condition ◽

Skin Barrier Function ◽

Keywords Atopic Dermatitis ◽

Immunological Dysfunction

Atopic dermatitis (AD) is a pathological skin condition with complex aetiological mechanisms that are difficult to fully understand. Scientific evidence suggests that of all the causes, the impairment of the skin barrier and cutaneous dysbiosis together with immunological dysfunction can be considered as the two main factors involved in this pathological skin condition. The loss of the skin barrier function is often linked to dysbiosis and immunological dysfunction, with an imbalance in the ratio between the pathogen Staphylococcus aureus and/or other microorganisms residing in the skin. The bibliographic research was conducted on PubMed, using the following keywords: ‘atopic dermatitis’, ‘bacterial therapy’, ‘drug delivery system’ and ‘alternative therapy’. The main studies concerning microbial therapy, such as the use of bacteria and/or part thereof with microbiota transplantation, and drug delivery systems to recover skin barrier function have been summarized. The studies examined show great potential in the development of effective therapeutic strategies for AD and AD-like symptoms. Despite this promise, however, future investigative efforts should focus both on the replication of some of these studies on a larger scale, with clinical and demographic characteristics that reflect the general AD population, and on the process of standardisation, in order to produce reliable data.

Download Full-text

Dietary Ceramide Prepared from Soy Sauce Lees Improves Skin Barrier Function in Hairless Mice

Journal of Oleo Science ◽

10.5650/jos.ess21128 ◽

2021 ◽

Author(s):

Kazushi Ohta ◽

Shinobu Hiraki ◽

Masakatsu Miyanabe ◽

Tatsuro Ueki ◽

Yuki Manabe ◽

...

Keyword(s):

Barrier Function ◽

Skin Barrier ◽

Soy Sauce ◽

Skin Barrier Function ◽

Hairless Mice

Download Full-text

Homemade Kefir Consumption Improves Skin Condition—A Study Conducted in Healthy and Atopic Volunteers

Foods ◽

10.3390/foods10112794 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2794

Author(s):

Emília Alves ◽

João Gregório ◽

André Rolim Baby ◽

Patrícia Rijo ◽

Luis M. Rodrigues ◽

...

Keyword(s):

Barrier Function ◽

Skin Diseases ◽

Skin Barrier ◽

Skin Condition ◽

Skin Barrier Function ◽

Fermented Foods ◽

Inflammatory Skin Diseases ◽

Health Promoting ◽

Relationship Of ◽

The Impact

Diet has a fundamental role in the homeostasis of bodily functions, including the skin, which, as an essential protective barrier, plays a crucial role in this balance. The skin and intestine appear to share a series of indirect metabolic pathways, in a dual relationship known as the “gut–skin axis”. Hence, the gut–skin axis might be receptive to modulation via dietary modification, where probiotics can be included, thus representing a potential therapeutic target in inflammatory skin diseases, such as atopic dermatitis (AD), in order to control and/or ameliorate symptoms. Kefir is one of the most ancient fermented foods, with probiotic characteristics that have been associated with a wide variety of health-promoting benefits, and it presents a microbiological diversity that makes its application as a probiotic in the gut–skin relationship of the utmost interest. However, the impact of a diet containing kefir on skin health has yet to be reported in scientific literature. This study aimed to assess the impact of the intake of homemade kefir in the skin of healthy and atopic volunteers. The intervention resulted in a boost on barrier function in both skin types verified only in the respective kefir intake groups. An improvement in the degree of severity of AD was also confirmed for the kefir intake group. Atopic individuals may benefit from kefir intake, especially in regard to their skin hydration. Finally, the effects observed on skin barrier function in this study probably culminate from the effects of all the ingredients in kefir, including the complex microbiota, its metabolites and macro- and micronutrients resulting from the fermentation. This work opens the way for more advanced research on the impact of the probiotic kefir on cutaneous health, further clarifying its mechanism of action namely via gut–skin axis.

Download Full-text

Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

Current Developments in Nutrition ◽

10.1093/cdn/nzaa045_020 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 387-387

Author(s):

Barbara Davis ◽

Dong Ju Son ◽

Jae Chul Jung ◽

Yong Min Choi ◽

Hyeon Yeol Ryu ◽

...

Keyword(s):

Oral Administration ◽

Barrier Function ◽

Collagen Synthesis ◽

Skin Barrier ◽

Human Keratinocytes ◽

Water Evaporation ◽

Skin Barrier Function ◽

Type I ◽

Skin Damage ◽

Hairless Mice

Abstract Objectives The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. Methods The ability for repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. To complement this work, and better understand the mechanism(s) through which this dietary ingredient works, changes in procollagen, hyaluronic acid (HA) and matrix metalloproteinase-1 (MMP-1) levels were assessed in response to UVB treatment in the presence and absence of WEO. Results The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, HA, and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of MMP-1, since its induction by UVB treatment was diminished in treated CCD-986sk cells. Conclusions Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption. Funding Sources Funding for this research was provided by the Life Science Research Institute, Novarex Co., Ltd.

Download Full-text