scholarly journals Reciprocal control of membrane permeability of transformed cultures of mouse cell lines by external and internal ATP.

1979 ◽  
Vol 254 (3) ◽  
pp. 708-714 ◽  
Author(s):  
E. Rozengurt ◽  
L.A. Heppel
Keyword(s):  
Membrane Permeability ◽  
Cell Lines ◽  
Mouse Cell ◽  
Transformed Cultures

scholarly journals The human TCF-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-lineage lymphocytes

Blood ◽  
1995 ◽  
Vol 86 (8) ◽  
pp. 3050-3059 ◽  
Author(s):  
J Castrop ◽  
D van Wichen ◽  
M Koomans-Bitter ◽  
M van de Wetering ◽  
R de Weger ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Nuclear Dna ◽  
Mouse Cell ◽  
Lymphoid Tissues ◽  
Sequence Motif ◽  
Knock Out ◽  
Putative Transcription Factor ◽  
Knock Out Mice ◽  
T Cell Malignancies

Abstract The TCF-1 gene encodes a putative transcription factor with affinity for a sequence motif occurring in a number of T-cell enhancers. TCF-1 mRNA was originally found to be expressed in a T cell-specific fashion within a set of human and mouse cell lines. In contrast, expression reportedly occurs in multiple nonlymphoid tissues during murine embryogenesis. We have now raised a monoclonal antibody to document expression and biochemistry of the human TCF-1 protein. As expected, the TCF-1 protein was detectable only in cell lines of T lineage. Its expression was always restricted to the nucleus. Immunohistochemistry on a panel of human tissues revealed that the TCF-1 protein was found exclusively in thymocytes and in CD3+ T cells in peripheral lymphoid tissues. Western blotting yielded a set of bands ranging from 25 kD to 55 kD, resulting from extensive alternative splicing. The TCF-1 protein was detectable in all samples of a set of 22 T-cell malignancies of various stages of maturation, but was absent from a large number of other hematologic neoplasms. These observations imply a T cell-specific function for TCF-1, a notion corroborated by recent observations on Tcf-1 knock-out mice. In addition, these results indicate that nuclear TCF-1 expression can serve as a pan-T-lineage marker in the diagnosis of lymphoid malignancies.

Chromosome Analysis of Two Related Heteroploid Mouse Cell Lines by Quinacrine Fluorescence

1973 ◽  
Vol 12 (1) ◽  
pp. 263-274
Author(s):  
P. W. ALLDERDICE ◽  
O. J. MILLER ◽  
D. A. MILLER ◽  
D. WARBURTON ◽  
P. L. PEARSON ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chromosome Analysis ◽  
Mouse Cell ◽  
Previous Method ◽  
Structural Rearrangements ◽  
Metaphase Chromosomes ◽  
Detailed Characterization ◽  
Banding Patterns ◽  
Fluorescent Banding ◽  
Quinacrine Fluorescence

The fluorescent banding patterns of quinacrine-stained metaphase chromosomes have been studied in 2 related mouse cell lines, A9 and a malignant derivative of A9, A9HT. In both cell lines virtually every chromosome has a distinctive banding pattern which permits its recognition. More than three quarters of the chromosomes have structural rearrangements, but the origin of nearly two thirds of the chromosomes could be determined by their banding patterns. The quinacrine fluorescence technique permits far more detailed characterization and comparison of heteroploid cell lines than any previous method. A9 and A9HT are karyologically quite similar, with many of the same marker chromosomes. There are, however, characteristic differences. A9HT, although it has a smaller average number of chromosomes per cell, appears to be more heterogeneous.

scholarly journals Effect of Mansoa alliacea (Bignonaceae) leaf extract on embryonic and tumorigenic mouse cell lines

2015 ◽  
Vol 9 (29) ◽  
pp. 799-805 ◽  
Author(s):  
M Towne Camden ◽  
F Dudt Jan ◽  
B Ray Durwood
Keyword(s):  
Cell Lines ◽  
Leaf Extract ◽  
Mouse Cell

Endogenous Superoxide Dismutase and Catalase Activities and Radiation Resistance in Mouse Cell Lines

1988 ◽  
Vol 53 (2) ◽  
pp. 283-289 ◽  
Author(s):  
C.A. Davy ◽  
Z. Tesfay ◽  
J. Jones ◽  
C. McCarthy ◽  
S. Ostrand-Rosenberg ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Cell Lines ◽  
Radiation Resistance ◽  
Mouse Cell

scholarly journals Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

2021 ◽  
Vol 12 ◽  
Author(s):  
Pengfei Li ◽  
Zhifang Hao ◽  
Jingyu Wu ◽  
Chen Ma ◽  
Yintai Xu ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Expression Profiles ◽  
Mouse Cell ◽  
Activated Macrophages ◽  
M1 Macrophages ◽  
Alternatively Activated Macrophages ◽  
Raw264.7 Macrophages ◽  
Protein Expression Profiles

Macrophages can be polarized into classically activated macrophages (M1) and alternatively activated macrophages (M2) in the immune system, performing pro-inflammatory and anti-inflammatory functions, respectively. Human THP-1 and mouse RAW264.7 cell line models have been widely used in various macrophage-associated studies, while the similarities and differences in protein expression profiles between the two macrophage models are still largely unclear. In this study, the protein expression profiles of M1 and M2 phenotypes from both THP-1 and RAW264.7 macrophages were systematically investigated using mass spectrometry-based proteomics. By quantitatively analyzing more than 5,000 proteins among different types of macrophages (M0, M1 and M2) from both cell lines, we identified a list of proteins that were uniquely up-regulated in each macrophage type and further confirmed 43 proteins that were commonly up-regulated in M1 macrophages of both cell lines. These results revealed considerable divergences of each polarization type between THP-1 and RAW264.7 macrophages. Moreover, the mRNA and protein expression of CMPK2, RSAD2, DDX58, and DHX58 were strongly up-regulated in M1 macrophages for both macrophage models. These data can serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.

scholarly journals Cytotoxic effect of Aeruginosin-865, capsaicin, and resveratrol on mouse cell lines of different origin

2021 ◽  
Vol 90 (3) ◽  
pp. 307-314
Author(s):  
Ivana Veselá ◽  
Jose Cheel ◽  
Jaroslava Tomenendálová ◽  
Pavla Hamouzová ◽  
Pavel Hrouzek ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cytotoxic Effect ◽  
Mouse Cell ◽  
Tumour Cells ◽  
Normal Cells ◽  
Hepatotoxic Effect ◽  
Anticancer Treatment ◽  
Mouse Cells ◽  
Different Origin ◽  
Anticancer Drug Development

The aim of the study was to compare the effects of three natural bioactive compounds (and their combinations) on normal vs. tumour-transformed mouse cells. The cytotoxic effect of Aeruginosin-865 (Aer), capsaicin (Cap), resveratrol (Res) and their combinations was evaluated on normal hepatocytes (AML) and tumour cells derived from livers (Hepa) and kidneys (Renca). Various concentrations from 25 to 200 μM of tested substances were used. Only the Aer + Res combination and a low concentration of Res had a significant cytotoxic effect on Hepa and Renca and no significant cytotoxic effect on AML. Cap had a significant cytotoxic effect on all tested cell lines, but tumour-derived cells showed higher resistance than AML. A significantly increased cytotoxicity was found in the combination of Cap + Res compared to each substance alone. All types of cells showed similar sensitivity to the cytotoxic effect of Cap + Res. Because of a possible hepatotoxic effect, we recommend further investigations into side-effects of Cap + Res. No cytotoxic effect was described in Cap + Aer or in Aer alone. Only substances with a significant cytotoxic effect on tumour cells and no cytotoxic effect on normal cells can be potentially used in anticancer treatment. According to the results, only Res or the combination of Aer + Res can be recommended for further evaluation in the process of new anticancer drug development. The potential hepatotoxic effect of Cap + Res can significantly limit the utilisation of these substances in anticancer treatment.

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