A METHOD FOR THE DETERMINATION OF GENTISIC ACID IN SERUM

The highly overlapping chromatograms and spectra of the major metabolites of asprin, salicylic acid (SA) and gentisic acid (GA) in the presence of uncalibrated and interfering pyrocatechuic acid (PA) were resolved using HPLC-DAD combined with SWATLD algorithm. The elution time was set from 0.6628 min to 0.9495 min and the detection wavelength was chosen from 268 nm to 332 nm. Both SA and GA were determined simultaneously in aqueous solution with their recoveries were (100.5 ± 6.7) % and (109.2 ± 5.3) %, respectively. The satisfying results indicate this method can be easily performed and applied to solving second-order calibration problems quickly and accurately.

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A Comparison of the Determination of Gentisic Acid in Blood by Means of the Ferric Ion-Gentisate Complex With and Without the Presence of Ferrous Ions *

Journal of the American Pharmaceutical Association (Scientific ed ) ◽

10.1002/jps.3030430118 ◽

1954 ◽

Vol 43 (1) ◽

pp. 50-52 ◽

Cited By ~ 1

Author(s):

Mary Cass ◽

John G. Wagner

Keyword(s):

Ferric Ion ◽

Ferrous Ions ◽

Gentisic Acid

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Determination of Aspirin, Salicylic Acid, Salicyluric Acid, and Gentisic Acid in Human Plasma and Urine by High Pressure Liquid Chromatography

Analytical Letters ◽

10.1080/00032717908055714 ◽

1979 ◽

Vol 12 (6) ◽

pp. 629-640 ◽

Cited By ~ 37

Author(s):

Edwin N. Amick ◽

William D. Mason

Keyword(s):

High Pressure ◽

Salicylic Acid ◽

Liquid Chromatography ◽

Human Plasma ◽

High Pressure Liquid Chromatography ◽

Gentisic Acid ◽

Salicyluric Acid ◽

Human Plasma And Urine

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Determination of each constituent in mixtures of catechol and protocatechuic acid, quinol and gentisic acid, phenol and p-hydroxybenzoic acid, and pyrogallol and gallic acid

Analytical Biochemistry ◽

10.1016/0003-2697(69)90165-1 ◽

1969 ◽

Vol 28 ◽

pp. 139-149 ◽

Cited By ~ 5

Author(s):

D.J.W. Grant ◽

J.C. Patel

Keyword(s):

Gallic Acid ◽

Protocatechuic Acid ◽

Hydroxybenzoic Acid ◽

Gentisic Acid

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Determination of salicylate, gentisic acid and salicyluric acid in human urine by capillary electrophoresis with laser-induced fluorescence detection

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/s0378-4347(00)00507-7 ◽

2001 ◽

Vol 752 (1) ◽

pp. 17-31 ◽

Cited By ~ 47

Author(s):

Sandra Zaugg ◽

Xin Zhang ◽

Jonathan Sweedler ◽

Wolfgang Thormann

Keyword(s):

Capillary Electrophoresis ◽

Fluorescence Detection ◽

Human Urine ◽

Laser Induced Fluorescence ◽

Gentisic Acid ◽

Laser Induced Fluorescence Detection ◽

Salicyluric Acid

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Simultaneous determination of gallic acid and gentisic acid in organic anion transporter expressing cells by liquid chromatography–tandem mass spectrometry

Journal of Chromatography B ◽

10.1016/j.jchromb.2013.08.024 ◽

2013 ◽

Vol 937 ◽

pp. 91-96 ◽

Cited By ~ 14

Author(s):

Li Wang ◽

Matthew S. Halquist ◽

Douglas H. Sweet

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Organic Anion ◽

Organic Anion Transporter ◽

Tandem Mass ◽

Gentisic Acid ◽

Chromatography Tandem Mass Spectrometry ◽

Anion Transporter ◽

Liquid Chromatography Tandem Mass

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Determination of citrate, inositol and gentisic acid in a pharmaceutical diagnostic formulation by ion-moderated partition chromatography

Journal of Chromatography A ◽

10.1016/0021-9673(94)00633-k ◽

1995 ◽

Vol 692 (1-2) ◽

pp. 203-205 ◽

Cited By ~ 4

Author(s):

Russell W. Chong ◽

Brenda J. Moore

Keyword(s):

Partition Chromatography ◽

Gentisic Acid

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Determination of Salicylic and Gentisic Acids Using HPLC-DAD Combined with Second-Order Calibration Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.2068 ◽

2012 ◽

Vol 554-556 ◽

pp. 2068-2072

Author(s):

Jian Qi Sun ◽

De Si Sun ◽

Guo Chao Li

Keyword(s):

Aqueous Solution ◽

Salicylic Acid ◽

Calibration Method ◽

Second Order ◽

Gentisic Acid ◽

Elution Time ◽

Second Order Calibration

The highly overlapped chromatograms and spectra of salicylic acid (SA), gentisic acid (GA) and uncalibrated interferent, pyrocatechuic acid (PA) were resolved using HPLC-DAD combined with a novel second-order calibration method. Their elution time was set from 0.6628 min to 0.9495 min and the detection wavelength was chosen from 268 nm to 332 nm. Both SA and GA were determined simultaneously in aqueous solution with their recoveries were (101.0 ± 7.1) % and (105.8 ± 5.6) %, respectively. The satisfying results demonstrate this method can be easily performed and applied to solving second-order calibration problems quickly and accurately.

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