Lauroyl sarcosine: a weak cation-exchange reagent for on-column modification of reversed-phase material

1987 ◽  
Vol 393 (2) ◽  
pp. 441-446 ◽  
Author(s):  
Ivan N. Mefford
Keyword(s):  
Cation Exchange ◽  
Reversed Phase ◽  
Weak Cation Exchange ◽  
Lauroyl Sarcosine

scholarly journals Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMS

2018 ◽  
Vol 17 (11) ◽  
pp. 3791-3800 ◽  
Author(s):  
Andrea F. G. Gargano ◽  
Jared B. Shaw ◽  
Mowei Zhou ◽  
Christopher S. Wilkins ◽  
Thomas L. Fillmore ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Reversed Phase ◽  
Separation Capacity ◽  
Weak Cation Exchange ◽  
Reversed Phase Lc

scholarly journals Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads

2015 ◽  
Vol 35 (3) ◽  
Author(s):  
Wei-Peng Jiang ◽  
Zhen Wang ◽  
Li-Xin Xu ◽  
Xin Peng ◽  
Feng Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cation Exchange ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Magnetic Beads ◽  
Diagnostic Model ◽  
Finger Print ◽  
Whole Saliva ◽  
Weak Cation Exchange

Whole saliva (WS) was used for diagnosis of oral squamous cell carcinoma (OSCC); two polypeptides may be used for OSCC diagnosis.

scholarly journals The use of LC tandem mass spectrometry as part of a workflow for the screening and identification of hemoglobin variants. Characterization of Hb Ullevaal as an example

2017 ◽  
Vol 41 (3) ◽  
Author(s):  
Matthias Weber ◽  
Julia J.M. Eekels
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Cation Exchange ◽  
Peripheral Blood ◽  
Globin Gene ◽  
Reversed Phase ◽  
Exchange Chromatography ◽  
South Florida ◽  
Tandem Mass ◽  
Genetic Sequencing

AbstractBackground:About 2/3 of the hemoglobin (Hb) variants do not show a charge difference to the wildtype entity but most of them differ in hydrophobicity. In addition to cation exchange chromatography, globin differentiation by liquid chromatography-tandem mass spectrometry (MS) was introduced. Hb Ullevaal was chosen as one example to demonstrate the performance of the approach.Methods:Screening for Hb variants was performed using cation exchange HPLC. For globin separation reversed phase-LC/MS was performed. Tryptic digests of variants were separated on RP-HPLC with or without CID-fragmentation and database search for identification of mutation bearing fragments. Sequencing of the β-globin gene has been performed.Results:HbS, HbC, HbE, Hb South Florida and Hb Ullevaal show typical and distinct patterns in the globin LC/MS according to the theoretical protein data. The tryptic digest of Hb Ullevaal resulted in the identification of the respective mutated peptide βT9, which was confirmed by genetic sequencing.Conclusions:By the application of globin-LC/MS two more dimensions for the Hb identification are added, hydropathicity and protein mass. With this workflow as screening procedure for Hb variants it is expected to be able to detect and identify the majority of variants with the exception of highly unstable variants, which cannot be determined in the peripheral blood at all. A negative result makes the presence of a significant Hb variant in the peripheral blood improbable.

Weak cation-exchange restricted-access material for on-line purification of basic drugs in plasma

2008 ◽  
Vol 1190 (1-2) ◽  
pp. 8-13 ◽  
Author(s):  
Yoshiaki Sato ◽  
Eiichi Yamamoto ◽  
Susumu Takakuwa ◽  
Takashi Kato ◽  
Naoki Asakawa
Keyword(s):  
Cation Exchange ◽  
Basic Drugs ◽  
Restricted Access ◽  
Restricted Access Material ◽  
Weak Cation Exchange ◽  
On Line
Sign in / Sign up

Export Citation Format

Share Document