Gas chromatographic analysis of free fatty acids and fatty acid salts extracted with neutral and acidified dichloromethane from office floor dust

1998 ◽  
Vol 814 (1-2) ◽  
pp. 161-170 ◽  
Author(s):  
Per Axel Clausen ◽  
Ken Wilkins ◽  
Peder Wolkoff
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Chromatographic Analysis ◽  
Gas Chromatographic Analysis ◽  
Fatty Acid Salts ◽  
Floor Dust ◽  
Acid Salts

Development of an Assay to Determine the Amount of Ca-Fatty Acid Soaps in Feces

2020 ◽  
Author(s):  
Natascha Stroebinger ◽  
Shane M Rutherfurd ◽  
Sharon J Henare ◽  
Paul J Moughan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Room Temperature ◽  
Degree Of Saturation ◽  
Acid Soap ◽  
Fatty Acid Salts ◽  
Almost All ◽  
Chain Lengths ◽  
Acid Salts

Abstract Background The quantification of fecal Ca-fatty acid soaps is important to understand how fatty acids behave in the gastrointestinal tract. Objective As current methods to extract Ca-fatty acid soaps from feces give low recoveries an accurate assay to determine the amount of fatty acid soaps in feces was developed. Method Ca-fatty acid soaps are determined indirectly after non-soap fatty acid compounds have been extracted from the feces. Synthetic Ca-fatty acid soaps of different chain lengths (C12–C18) and degree of saturation (C18:0–C18:2) were incubated with several solvents to find the solvents that least-solubilize the Ca-fatty acid soaps. A three-step extraction was devised using extractions with hexane, hexane-isopropanol and water either at room temperature or at 60°C, 37°C, or 80°C, respectively. Feces were spiked with free fatty acids, Ca-fatty acid soaps, Na-fatty acid salts, and phospholipids. Results All of the free fatty acids and phospholipids and almost all of the Na-fatty acid salts were removed and 98% of Ca-lauric acid soap, 99% of Ca-stearic acid soap, and 93% of oleic acid soap were recovered. Conclusions The method is suitable for determining fatty acids in the form of Ca-fatty acid soaps in feces. Highlights New method to determine fecal Ca-fatty acid soaps. Consistent and high recovery of fatty acid-soaps.

Gas chromatographic analysis of free fatty acids. Part 2: Determination of free fatty acids during anaerobic putrefaction

1987 ◽  
Vol 7 (5) ◽  
pp. 479-483 ◽  
Author(s):  
S. Pörschmann ◽  
J. Pörschmann ◽  
L. Liebetrau ◽  
I. Kühn ◽  
R. Pätz
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Chromatographic Analysis ◽  
Gas Chromatographic Analysis

scholarly journals Analisis Asam Lemak dalam Minyak Kelapa Murni Menggunakan Derivatisasi Katalis Basa

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 29
Author(s):  
Mevy Alvionita Abast ◽  
Harry S. J. Koleangan ◽  
Julius Pontoh
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chromatographic Analysis ◽  
Coconut Oil ◽  
Fatty Acid Analysis ◽  
Virgin Coconut Oil ◽  
Gas Chromatographic Analysis ◽  
External Standard ◽  
Naoh Solution

Telah dilakukan penelitian mengenai analisis asam lemak dari minyak kelapa murni dengan menggunakan teknik derivatisasi basa dan dilanjutkan dengan analisis metil ester asam lemak dengan kromatografi gas. Sampel minyak kelapa diderivatisasi dengan menggunakan larutan NaOH dalam metanol, dipanaskan dan dinetralisir dengan HCl.  Hasil analisis menunjukan bahwa metode ini sangat baik oleh karena seluruh minyak terderivatisasi.  Kandungan asam lemak yang dilakukan dengan teknik external standard menunjukan bahwa konsentrasi masing – masing asam lemak pada sampel minyak kelapa murni adalah kaprilat 6,11 mg/mL, kaprat 3,85 mg/mL, laurat 25,75 mg/mL, miristat 9,84 mg/mL, palmitat 4,45 mg/mL, stearat 1,36 mg/mL, oleat 3,94 mg/mL, linoleat 0,69 mg/mL.A research had been conducted to analyze the fatty acid in virgin coconut oil using derivatization with base catalizator followed by gas chromatographic analysis.  Oil sample was derivatized using NaOH solution in methanol, heated and netralized by HCl.  The result showed that this derivatization method was very good because all the oil sample was derivatized.  The fatty acid analysis determined using external standard showed concentration  of each fatty acids in virgin coconut oil sample is caprylic 6,11 mg/mL, capric 3,85 mg/mL, lauric 25,75 mg/mL, myristic 9,84 mg/mL, palmitic 4,45 mg/mL, stearic 1,36 mg/mL, oleic 3,94 mg/mL, linoleic 0,69 mg/mL.

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