scholarly journals Lipid transfers between reconstituted high density lipoprotein complexes and low density lipoproteins: effects of plasma protein factors

1988 ◽  
Vol 29 (10) ◽  
pp. 1349-1357
Author(s):  
A Jonas ◽  
K E Kézdy ◽  
M I Williams ◽  
K A Rye
Keyword(s):  
High Density Lipoprotein ◽  
Plasma Protein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Low Density

Evaluation of commercial heparin preparations for use in the heparin-Mn2+ method for measuring cholesterol in high-sensity lipoprotein.

1979 ◽  
Vol 25 (7) ◽  
pp. 1309-1313 ◽  
Author(s):  
C Mayfield ◽  
G R Warnick ◽  
J J Albers
Keyword(s):  
High Density Lipoprotein ◽  
Intestinal Mucosa ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Mean Values ◽  
Significant Difference

Abstract Commercial heparin preparations (18 lots) from seven manufacturers were compared in the heparin-Mn2+ procedure for high-density-lipoprotein cholesterol quantitation. With normotriglyceridemic samples, 16 heparin lots, isolated from porcine intestinal mucosa, gave mean values for supernatant cholesterol that did not differ statistically; all were within 7 mg/L. Two heparin preparations from bovine lung gave results that were slightly (16 mg/L, average) but significantly (p less than 0.005) lower. With hypertriglyceridemic samples, we observed greater variation in supernatant cholesterol among the heparin preparations, which was ascribable to variable sedimentation by centrifugation of very-low-density and low-density lipoproteins precipitated by heparin-Mn2+ treatment. If the precipitated lipoproteins were completely removed by an ultrafiltration procedure, we saw no significant difference among the heparin preparations for results with hypertriglyceridemic samples.

An alpha slow-moving high-density-lipoprotein subfraction in serum of a patient with radiation enteritis and peritoneal carcinosis.

1989 ◽  
Vol 35 (4) ◽  
pp. 674-678
Author(s):  
J Peynet ◽  
A Legrand ◽  
B Messing ◽  
F Thuillier ◽  
F Rousselet
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Radiation Enteritis ◽  
Low Density ◽  
Peritoneal Carcinosis ◽  
Very Low Density Lipoproteins ◽  
Slow Moving

Abstract An alpha slow-moving high-density-lipoprotein (HDL) subfraction was seen in a patient presenting with radiation enteritis and peritoneal carcinosis, who was given long-term cyclic parenteral nutrition. This subfraction, observed in addition to normal HDL, was precipitated with low-density lipoproteins (LDL) and very-low-density lipoproteins (VLDL) by sodium phosphotungstate-magnesium chloride. The patient's serum lipoproteins were analyzed after fractionation by density gradient ultracentrifugation. The alpha slow-moving HDL floated in the ultracentrifugation subfractions with densities ranging from 1.028 to 1.084 kg/L, and their main apolipoproteins included apolipoprotein E in addition to apolipoprotein A-I. These HDL were larger than HDL2. The pathogenesis of this unusual HDL subfraction is hypothesized.

Evaluation of the Polyethylene Glycol Precipitation Method for the Estimation of High-Density Lipoprotein Cholesterol

1981 ◽  
Vol 18 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Catherine J Briggs ◽  
Deborah Anderson ◽  
P Johnson ◽  
T Deegan
Keyword(s):  
Polyethylene Glycol ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Selective Precipitation

Treatment of fresh sera with polyethylene glycol 6000 at a final concentration of 100 g/l produced selective precipitation of low-density lipoproteins with only traces of contamination with high-density lipoproteins, as determined by electroimmunoassay using antisera to human α1-lipoprotein and human β-lipoprotein. Supernatants collected for high-density lipoprotein-cholesterol estimation were free from low-density lipoproteins. Precipitates sedimented readily from specimens with high triglyceride contents, and secondary precipitation during enzymatic cholesterol determinations was absent. Values obtained by this method correlated well with those obtained by precipitation of low-density lipoproteins with heparin and manganous ions at concentrations optimal for discrete separation of lipoprotein classes (r = 0·975; P<0·001).

Loss of A-Apoprotein Immunoreactivity during High-Density Lipoprotein Separation

1981 ◽  
Vol 18 (5) ◽  
pp. 308-313 ◽  
Author(s):  
P Johnson ◽  
R A Muirhead ◽  
T Deegan
Keyword(s):  
High Density Lipoprotein ◽  
Surface Structure ◽  
Analytical Ultracentrifugation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Low Density ◽  
Apoprotein B

By use of an electroimmunoassay, concentrations of A-apoproteins were estimated in serum and in corresponding apoprotein fractions isolated by ultracentrifugation. These values were compared with high-density lipoprotein concentrations determined by analytical ultracentrifugation. Concentrations of A-apoproteins estimated in serum were considerably higher than in isolated high-density lipoprotein fractions. These discrepancies could not be accounted for entirely by material losses into other fractions during ultracentrifugal fractionation. No comparable differences in apoprotein-B concentrations were observed during the ultracentrifugal separation of low-density lipoprotein. Concentrations of A-apoproteins estimated in the residual serum after precipitation of low-density lipoproteins by heparin and manganous ions were also lower than in the corresponding whole sera. The discrepancies persisted after treatment of serum and isolated fractions with tetramethylurea, urea (9 mol/l), and by heating at 52°C for 3 hours. It is considered that separation by ultracentrifugation induces subtle alterations in the surface structure of the lipoprotein species which give rise to changes in immunoreactivity.

Homogeneous assay for direct determination of high-density lipoprotein cholesterol evaluated

1996 ◽  
Vol 42 (3) ◽  
pp. 424-429 ◽  
Author(s):  
M Nauck ◽  
W März ◽  
B Haas ◽  
H Wieland
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Apo B ◽  
Homogeneous Assay

Abstract We evaluated a new homogeneous assay for quantifying high-density lipoprotein cholesterol (HDL-C). The assay included four reagents: polyethylene glycol for "wrapping" chylomicrons, very-low-density lipoproteins (VLDL), and low-density lipoproteins (LDL); antibodies specific for apolipoprotein (apo) B and apo C-III to produce aggregates of chylomicrons, VLDL, and LDL; enzymes for the enzymatic cholesterol determination of the noncomplexed lipoproteins with 4-aminoantipyrine as the color reagent; and guanidine salt to stop the enzymatic reaction and to solubilize the complexes of apo B-containing lipoproteins, which would otherwise interfere with the reading of absorbance. The total CVs of the new method ranged between 2.4% and 8.4%. The HDL-C values (y) were in good agreement with those by a comparison phosphotungstic acid/MgCl2 method (x): y= 0.987x + 17.2 mg/L (68th percentile of the residuals on the regression line= 21.49, r= 0.970). At triglyceride concentrations of 20 g/L (Intralipid) the homogeneous HDL-C concentrations increased by 2%. Hemoglobin markedly increased the results, whereas bilirubin reduced them. The homogeneous HDL-C assay was easy to handle and allows full automation. This test should considerably facilitate the screening of individuals at an increased risk of cardiovascular disease.

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