A technique for the cannulation and perfusion of isolated rat epididymal fat pad

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

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Stimulatory action of cycloheximide on glucose metabolism in the rat epididymal fat pad

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)41719-5 ◽

1977 ◽

Vol 18 (1) ◽

pp. 93-98

Author(s):

J A García-Sáinz ◽

E Piña ◽

V Chagoya de Sánchez

Keyword(s):

Glucose Metabolism ◽

Fat Pad ◽

Stimulatory Action ◽

Epididymal Fat

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Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Journal of Visualized Experiments ◽

10.3791/54995 ◽

2017 ◽

Cited By ~ 4

Author(s):

Kai Wang ◽

Xi Wang ◽

Cheng-sheng Han ◽

Liang-yi Chen ◽

Ying Luo

Keyword(s):

Diabetic Mice ◽

Fat Pad ◽

Epididymal Fat

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Acetate-1-C14 metabolism of white fat from hamsters in cold exposure and hibernation

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1963.205.5.905 ◽

1963 ◽

Vol 205 (5) ◽

pp. 905-908 ◽

Cited By ~ 80

Author(s):

Joan Baumber ◽

Arliss Denyes

Keyword(s):

Cold Exposure ◽

Room Temperature ◽

Fat Pad ◽

Golden Hamsters ◽

Epididymal Fat ◽

Cold Room ◽

White Fat

Incorporation of C14 from acetate-1-C14 into lipid and CO2 by epididymal fat from golden hamsters kept at room temperature, acclimated to 5 ± 1 C, in hibernation and arousing from hibernation, was measured in vitro at 37 C. Summer and winter series were compared. The C14O2 production by tissue from control and acclimated animals was similar but the C14O2 production of tissue from hibernating and arousing hamsters was significantly greater than that from acclimated animals. There was a large increase in the lipid-C14 of tissue from cold-acclimated animals and this increase persisted into hibernation but was slightly depressed in tissue from arousing animals. Many acclimated and all hibernating hamsters had involuted testes and a greater incorporation of C14 into lipid than those with noninvoluted testes. A greater percentage of hamsters hibernated in the cold room during the winter and at this time the incorporation of C14 into lipid by the fat pad was greater than in the summer.

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Effect of cold stress on lipogenesis by adipose tissue

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1960.198.5.1123 ◽

1960 ◽

Vol 198 (5) ◽

pp. 1123-1125 ◽

Cited By ~ 71

Author(s):

E. J. Masoro ◽

Edith Porter ◽

Judith Patkin

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Cold Stress ◽

Incubation Medium ◽

Acetate Metabolism ◽

Hepatic Lipogenesis ◽

Acetate Oxidation ◽

Fat Pad ◽

Striking Finding ◽

Epididymal Fat

The effect of cold stress on acetate metabolism by adipose tissue was investigated. Cold stress did not affect the ability of the epididymal fat pad to oxidize acetate-1-C14 to C14O2. The addition of unlabeled glucose to the incubation medium did not influence the rate of acetate oxidation in the case of adipose tissue obtained from either control or cold-stressed rats. In the absence of unlabeled glucose, more fatty acids from acetate-1-C14 were synthesized by the adipose tissue from control rats than by that from cold-stressed rats, although very little was synthesized by either. The addition of unlabeled glucose to the incubation medium at the physiologic concentration of 100 mg % caused the adipose tissue from both normal and cold-stressed rats to form fatty acids at high rates. It is a striking finding that cold stress, which almost abolishes hepatic lipogenesis, does not appreciably alter adipose tissue lipogenesis.

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Alteration of body composition in rats: Effect of organic chromium and L-carnitine

Acta Veterinaria Hungarica ◽

10.1556/004.49.2001.4.3 ◽

2001 ◽

Vol 49 (4) ◽

pp. 385-398 ◽

Cited By ~ 3

Author(s):

S. Fekete ◽

I. Szakáll ◽

Emma Kósa ◽

Emese Andrásofszky ◽

Kinga Fodor ◽

...

Keyword(s):

Electrical Conductivity ◽

Body Composition ◽

Protein Level ◽

Close Correlation ◽

The Body ◽

Fat Pad ◽

Fat Percentage ◽

Epididymal Fat ◽

Total Body ◽

Protein Supply

A growth trial was carried out to test the effect of organic, trivalent chromium and L-carnitine on the body composition of growing rats. At the same time, an evaluation of different measurement methods (weight of epididymal fat pad, adipocyte morphometry, total body electrical conductivity) was performed. Outbred Wistar rats of 30 days of age were fed diets of different (0, 10 and 20%) protein level. The diets were supplemented with 4 mg/kg Cr as chromium nicotinate, and 100 mg/kg L-carnitine. The experimental feeding lasted 15 days, after a 5-day-long adjustment period. It was found that Cr addition increased feed intake. Both treatments caused changes in body composition, increasing fat and protein deposition. Organic chromium had no effect at either protein level, while L-carnitine improved the protein retention only at an optimum (20%) protein supply. No statistically significant correlation was found between total body electrical conductivity (TOBEC) and body composition, which could be attributed to the great individual differences. A close correlation was found among total body fat percentage, weight of epididymal fat pad and the adipocyte surface. The data suggest that there is an interaction between dietary protein supply and the effect of repartitioning agents.

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