Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture

1968 ◽  
Vol 35 (2) ◽  
pp. 291-301 ◽  
Author(s):  
Daryl K. Granner ◽  
Shin-ichi Hayashi ◽  
E. Brad Thompson ◽  
Gordon M. Tomkins
Keyword(s):  
Cell Culture ◽  
Tyrosine Aminotransferase ◽  
Dexamethasone Phosphate ◽  
Stimulation Of

scholarly journals Interleukin-8 and Growth-Regulated Oncogene Alpha Mediate Angiogenesis in Kaposi's Sarcoma

2002 ◽  
Vol 76 (22) ◽  
pp. 11570-11583 ◽  
Author(s):  
Brian R. Lane ◽  
Jianguo Liu ◽  
Paul J. Bock ◽  
Dominique Schols ◽  
Michael J. Coffey ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Interleukin 8 ◽  
Cellular Growth ◽  
Immunodeficiency Virus ◽  
Hiv 1 ◽  
Stimulation Of

ABSTRACT The development of the complex neoplasm Kaposi's sarcoma is dependent on infection with the Kaposi's sarcoma-associated herpesvirus (KSHV) and appears to be greatly enhanced by cytokines and human immunodeficiency virus type 1 (HIV-1) Tat. Interleukin-8 (IL-8) and growth-regulated oncogene alpha (GRO-α) are chemokines involved in chemoattraction, neovascularization, and stimulation of HIV-1 replication. We have previously demonstrated that production of GRO-α is stimulated by exposure of monocyte-derived macrophages (MDM) to HIV-1. Here we show that exposure of MDM to HIV-1, viral Tat, or viral gp120 leads to a substantial increase in IL-8 production. We also demonstrate that IL-8 and GRO-α are induced by KSHV infection of endothelial cells and are crucial to the angiogenic phenotype developed by KSHV-infected endothelial cells in cell culture and upon implantation into SCID mice. Thus, the three known etiological factors in Kaposi's sarcoma pathogenesis—KSHV, HIV-1 Tat, and cellular growth factors—might be linked, in part, through induction of IL-8 and GRO-α.

scholarly journals Regulation of angiopoietin-like protein 4/fasting-induced adipose factor (Angptl4/FIAF) expression in mouse white adipose tissue and 3T3-L1 adipocytes

2008 ◽  
Vol 100 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Sarah Dutton ◽  
Paul Trayhurn
Keyword(s):  
Skeletal Muscle ◽  
Cell Culture ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Culture Medium ◽  
Mrna Level ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Before And After ◽  
Stimulation Of

Angiopoietin-like protein 4 (Angptl4)/FIAF (fasting-induced adipose factor) was first identified as a target for PPAR and to be strongly induced in white adipose tissue (WAT) by fasting. Here we have examined the regulation of the expression and release of this adipokine in mouse WAT and in 3T3-L1 adipocytes. Angptl4/FIAF expression was measured by RT-PCR and real-time PCR; plasma Angptl4/FIAF and release of the protein in cell culture was determined by western blotting. The Angptl4/FIAF gene was expressed in each of the major WAT depots of mice, the mRNA level in WAT being similar to the liver and much higher (>50-fold) than skeletal muscle. Fasting mice (18 h) resulted in a substantial increase in Angptl4/FIAF mRNA in liver and muscle (9·5- and 21-fold, respectively); however, there was no effect of fasting on Angptl4/FIAF mRNA in WAT and the plasma level of Angptl4/FIAF was unchanged. The Angptl4/FIAF gene was expressed in 3T3-L1 adipocytes before and after differentiation, the level increasing post-differentiation; Angptl4/FIAF was released into the culture medium. Insulin, leptin, dexamethasone, noradrenaline, TNFα and several IL (IL-1β, IL-6, IL-10, IL-18) had little effect on Angptl4/FIAF mRNA levels in 3T3-L1 adipocytes. However, a major stimulation of Angptl4/FIAF expression was observed with rosiglitazone and the inflammatory prostaglandins PGD2 and PGJ2. Angptl4/FIAF does not act as an adipose tissue signal of nutritional status, but is markedly induced by fasting in liver and skeletal muscle.

scholarly journals Localized mechanical stimulation of single cells with engineered spatio-temporal profile

Lab on a Chip ◽  
2018 ◽  
Vol 18 (19) ◽  
pp. 2955-2965 ◽  
Author(s):  
M. Monticelli ◽  
D. S. Jokhun ◽  
D. Petti ◽  
G. V. Shivashankar ◽  
R. Bertacco
Keyword(s):  
Cell Culture ◽  
Mechanical Stimulation ◽  
Single Cells ◽  
Mechanical Stimuli ◽  
Temporal Profile ◽  
A Cell ◽  
Spatio Temporal ◽  
Stimulation Of

We introduce a new platform for mechanobiology based on active substrates, made of Fe-coated polymeric micropillars, capable to apply mechanical stimuli with tunable spatio-temporal profile on a cell culture.

An optimized model for hCG stimulation of human mural granulosa cell culture

2019 ◽  
Vol 19 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Libby Ophir ◽  
Yuval Yung ◽  
Gil Mordechai Yerushalmi ◽  
Micha Baum ◽  
Ronit Machtinger ◽  
...  
Keyword(s):  
Cell Culture ◽  
Granulosa Cell ◽  
Optimized Model ◽  
Stimulation Of

scholarly journals The effect of PEGylation on the stimulation of IL-1β by gold (Au) nanoshell/silica core nanoparticles

2016 ◽  
Vol 4 (9) ◽  
pp. 1650-1659 ◽  
Author(s):  
Hai T. Nguyen ◽  
Hong Shen
Keyword(s):  
Cell Culture ◽  
Peritoneal Cavity ◽  
Silica Core ◽  
Core Nanoparticles ◽  
Stimulation Of

PEGylation of Au nanoshell/silica core (GNS) particles reduces the aggregation in cell culture and the generation of IL-1β and the influx of neutrophils in the mouse peritoneal cavity.

Analysis of hormonal–dietary interactions and the role of gluconeogenesis in the regulation of tyrosine aminotransferase in rats

1969 ◽  
Vol 47 (11) ◽  
pp. 1053-1061 ◽  
Author(s):  
C. Mavrides ◽  
E. A. Lane
Keyword(s):  
Enzyme Activity ◽  
High Protein Diet ◽  
Tyrosine Aminotransferase ◽  
High Carbohydrate Diet ◽  
Protein Diet ◽  
Carbohydrate Diet ◽  
Hormonal Stimulation ◽  
High Carbohydrate ◽  
Hormonal Effect ◽  
Stimulation Of

The extent of stimulation of rat liver tyrosine aminotransferase by Cortisol and glucagon is inversely proportional to the basal activity of the enzyme in adrenalectomized and hypophysectomized rats. The basal activity is low in animals fed a high-carbohydrate diet leading to high hormonal stimulation of the enzyme, and high in animals fed a high-protein diet leading to minimal or no hormonal stimulation. The dietary modifications of the hormonal effects have been analyzed into two independent processes. Upon short-term starvation, the enzyme activity declines rapidly in animals previously fed a high-protein diet but rises rapidly in animals previously fed a high-carbohydrate diet. Superimposing a positive (hormonal) effect on these two diametrically opposite processes results in profound modification of the hormonal effect on the enzyme activity. The experiments further suggest that the regulation of tyrosine aminotransferase is coupled with gluconeogenic activity per se rather than with a primary hormonal action at the genetic level.

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