Spectral monitoring of biologically active solar UVB radiation using an in vitro model of vitamin D synthesis

Talanta ◽  
2000 ◽  
Vol 53 (1) ◽  
pp. 195-203 ◽  
Author(s):  
I Terenetskaya
Keyword(s):  
Vitamin D ◽  
In Vitro Model ◽  
Biologically Active ◽  
Uvb Radiation ◽  
Vitro Model ◽  
Spectral Monitoring

scholarly journals Use of an in vitro model of hepatic steatosis for studying the anti-oxidant and antisteatotic effects of fucoidan polysaccharides

2020 ◽  
Author(s):  
Zeinab El Rashed ◽  
Hala Khalife ◽  
Adriana Voci ◽  
Elena Grasselli ◽  
Laura Canesi ◽  
...  
Keyword(s):  
Marine Algae ◽  
In Vitro Model ◽  
Biological Effects ◽  
Biologically Active ◽  
Terrestrial Plants ◽  
Alcoholic Fatty Liver ◽  
Anti Oxidant ◽  
Vitro Model ◽  
Alcoholic Fatty Liver Disease

Non Alcoholic Fatty Liver Disease (NAFLD) is characterised by fat accumulation in hepatocytes in the form of triacyglycerols (TAGs) within cytosolic lipid droplets. Fucoidans (FUs) are biologically active polysaccharides usually isolated from brown marine algae, but recently identified also in terrestrial plants. In this study, we aimed to investigate the anti-oxidant and anti-steatotic effects of FUs purified from C. compressa, F. hermonis, and E. globulus. To this aim, we used a validated NAFLD in vitro model consisting of rat hepatoma FaO cells exposed to an oleate/palmitate mixture. Such a model is suitable for rapid investigation of direct effects of natural and artificial compounds, together with satisfying the strategy of 3Rs for laboratory use of animals. Our results indicated that all FUs display anti-oxidant and anti-steatotic activities. Steatotic FaO cells may be employed to further study the biological effects of FUs.

Vitamin D-regulated calcium transport in Caco-2 cells: unique in vitro model

1991 ◽  
Vol 260 (2) ◽  
pp. G207-G212 ◽  
Author(s):  
A. R. Giuliano ◽  
R. J. Wood
Keyword(s):  
Vitamin D ◽  
Cell Line ◽  
Calcium Transport ◽  
In Vitro Model ◽  
Colon Adenocarcinoma ◽  
Intestinal Cell ◽  
Maximal Velocity ◽  
Dependent Manner ◽  
Vitro Model

The human colon adenocarcinoma cell line Caco-2 is the only intestinal cell line to differentiate spontaneously in culture exhibiting structural and biochemical characteristics of mature enterocytes and to possess a vitamin D receptor in the fully differentiated state. Transepithelial calcium transport was characterized in differentiated Caco-2 cells grown on permeable filters supports to assess the potential utility of this cell line as an in vitro model to study 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]-induced calcium transport. Calcium transport was increased in a dose-dependent manner by 1,25(OH)2D3. Total calcium transport at different calcium concentrations could be fitted to a modified Michaelis-Menten equation containing a linear transport component. The maximum rate of saturable calcium transport was increased by 4.3-fold (P less than 0.005) in cells treated with 10(-8) M 1,25(OH)2D3. This treatment also increased the apparent buffer calcium concentration that results in half-maximal velocity from 0.4 to 1.3 mM but had no significant effect on nonsaturable calcium transport. Caco-2 cells grown on permeable filter supports provide a unique in vitro human cell culture model to study the mechanism of vitamin D-regulated transepithelial intestinal calcium transport.

Transport ofSchisandra chinensisextract and its biologically-active constituents across Caco-2 cell monolayers - an in-vitro model of intestinal transport

2008 ◽  
Vol 60 (3) ◽  
pp. 363-370 ◽  
Author(s):  
Vamsi L. M. Madgula ◽  
Bharathi Avula ◽  
Young W. Choi ◽  
Srinivas V. Pullela ◽  
Ikhlas A. Khan ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Intestinal Transport ◽  
Biologically Active ◽  
Active Constituents ◽  
Cell Monolayers ◽  
Vitro Model

The Effect of Vitamin D on Anti-HIV microRNAs and HIV Replication in Vitro Model

2020 ◽  
Vol 35 (3) ◽  
pp. 189-193
Author(s):  
Shohreh Shahmahmoodi ◽  
Maryam Yousefi ◽  
Yaghoob Mollaei-Kandelous ◽  
Hamideh Tabatabaie ◽  
Sayed Mahdi Marashi ◽  
...  
Keyword(s):  
Vitamin D ◽  
In Vitro Model ◽  
Hiv Replication ◽  
Vitro Model ◽  
Anti Hiv

scholarly journals The Vitamin D reduces HIV-1 infection of CD4+ T cells in an in vitro model

2015 ◽  
Vol 6 ◽  
Author(s):  
Villegas-Ospina Simon ◽  
Aguilar-Jiménez Wbeimar ◽  
Gonzalez Sandra ◽  
Zapata Wildeman ◽  
Saulle Irma ◽  
...  
Keyword(s):  
Vitamin D ◽  
T Cells ◽  
Cd4 T Cells ◽  
In Vitro Model ◽  
Vitro Model ◽  
Hiv 1

In Vitro Model of Vitamin D Synthesis by UV Radiation in an Australian Urban Environment

2010 ◽  
Vol 87 (2) ◽  
pp. 447-451 ◽  
Author(s):  
Alex McKinley ◽  
Monika Janda ◽  
Josephine Auster ◽  
Michael Kimlin
Keyword(s):  
Vitamin D ◽  
Urban Environment ◽  
Uv Radiation ◽  
In Vitro Model ◽  
Vitro Model

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Sign in / Sign up

Export Citation Format

Share Document