Mass spectrometry in structural and stereochemical problems CVIII. Deuterium isotope effects in site-specific mass spectrometric rearrangement processess

1966 ◽  
Vol 7 (19) ◽  
pp. 2183-2187 ◽  
Author(s):  
J.K. MacLeod ◽  
Carl Djerassi
Keyword(s):  
Mass Spectrometry ◽  
Isotope Effects ◽  
Mass Spectrometric ◽  
Specific Mass ◽  
Site Specific ◽  
Deuterium Isotope Effects

scholarly journals Site-specific glycan analysis of the SARS-CoV-2 spike

Science ◽  
2020 ◽  
pp. eabb9983 ◽  
Author(s):  
Yasunori Watanabe ◽  
Joel D. Allen ◽  
Daniel Wrapp ◽  
Jason S. McLellan ◽  
Max Crispin
Keyword(s):  
Immune Response ◽  
Immune Evasion ◽  
Vaccine Development ◽  
Mass Spectrometric ◽  
Cell Entry ◽  
Specific Mass ◽  
Site Specific ◽  
Humoral Immune ◽  
Mass Spectrometric Approach ◽  
A Site

The emergence of the betacoronavirus, SARS-CoV-2, the causative agent of COVID-19, represents a significant threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design.

ChemInform Abstract: NMR DETERMINATION OF SITE-SPECIFIC DEUTERIUM ISOTOPE EFFECTS

1982 ◽  
Vol 13 (45) ◽  
Author(s):  
D. M. GRANT ◽  
J. CURTIS ◽  
W. R. CROASMUN ◽  
D. K. DALLING ◽  
F. W. WEHRLI ◽  
...  
Keyword(s):  
Isotope Effects ◽  
Site Specific ◽  
Deuterium Isotope Effects

NMR determination of site-specific deuterium isotope effects

1982 ◽  
Vol 104 (16) ◽  
pp. 4492-4494 ◽  
Author(s):  
David M. Grant ◽  
Janet Curtis ◽  
William R. Croasmun ◽  
Don K. Dalling ◽  
Felix W. Wehrli ◽  
...  
Keyword(s):  
Isotope Effects ◽  
Site Specific ◽  
Deuterium Isotope Effects

The challenge of detecting modifications on proteins

2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska
Keyword(s):  
Mass Spectrometry ◽  
Protein Function ◽  
Chemical Properties ◽  
Lysine Acetylation ◽  
Mass Determination ◽  
Post Translational Modifications ◽  
Site Specific ◽  
The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

Confounding contaminants in mass spectrometric reaction monitoring

2018 ◽  
Author(s):  
Gilian T. Thomas ◽  
Landon MacGillivray ◽  
Natalie L. Dean ◽  
Rhonda L. Stoddard ◽  
Lars Yunker ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometric ◽  
Negative Ion ◽  
Reaction Monitoring ◽  
Schlenk Flask ◽  
Dynamic Analyses ◽  
Rubber Septa ◽  
Negative Ion Mode ◽  
Mode A

<p>Reactions carried out in the presence of rubber septa run the risk of additives being leached out by the solvent. Normally, such species are present at low enough levels that they do not interfere with the reaction significantly. However, when studying reactions using sensitive methods such as mass spectrometry, the appearance of even trace amounts of material can confuse dynamic analyses of reactions. A wide variety of additives are present in rubber along with the polymer: antioxidants, dyes, detergent, and vulcanization agents, and these are all especially problematic in negative ion mode. A redesigned Schlenk flask for pressurized sample infusion (PSI) is presented as a means of practically eliminating the presence of contaminants during reaction analyses.</p>

Sign in / Sign up

Export Citation Format

Share Document