In vitro Culture of Anthers and Stem Internodes of Lycopersicum peruvianum: Nuclear DNA Determination in Calli and Cytological Analysis of Regenerated Plants

1977 ◽  
Vol 82 (5) ◽  
pp. 377-388 ◽  
Author(s):  
G. Ancora ◽  
K. Sree Ramulu ◽  
M. Devreux
Keyword(s):  
In Vitro Culture ◽  
Nuclear Dna ◽  
Cytological Analysis ◽  
Regenerated Plants

In vitro culture of Aloe barbadensis Mill.: quantitative DNA variations in regenerated plants

Plant Science ◽  
1993 ◽  
Vol 91 (2) ◽  
pp. 223-229 ◽  
Author(s):  
A. Cavallini ◽  
L. Natali ◽  
G. Cionini ◽  
O. Sassoli ◽  
I. Castorena-Sanchez
Keyword(s):  
In Vitro Culture ◽  
Aloe Barbadensis ◽  
Regenerated Plants ◽  
Dna Variations

scholarly journals Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture

2000 ◽  
Vol 23 (2) ◽  
pp. 479-493 ◽  
Author(s):  
Guillermo Pratta ◽  
Roxana Zorzoli ◽  
Liliana Amelia Picardi
Keyword(s):  
Multivariate Analysis ◽  
In Vitro Culture ◽  
Principal Component ◽  
Eigenvalues And Eigenvectors ◽  
Morphometric Traits ◽  
Regenerated Plants ◽  
Phenotypic Structure ◽  
Regeneration Cycle ◽  
The Stability

The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.

scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

scholarly journals Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 154-155
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

In vitro culture of Aloe barbadensis Mill.: Morphogenetic ability and nuclear DNA content

Plant Science ◽  
1988 ◽  
Vol 55 (1) ◽  
pp. 53-59 ◽  
Author(s):  
I. Castorena Sanchez ◽  
L. Natali ◽  
A. Cavallin
Keyword(s):  
In Vitro Culture ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Aloe Barbadensis

scholarly journals Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 526
Author(s):  
Iwona Chłosta ◽  
Dagmara Kwolek ◽  
Elwira Sliwinska ◽  
Grzegorz Góralski ◽  
Marzena Popielarska-Konieczna
Keyword(s):  
Molecular Markers ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Dioecious Species ◽  
Culture Techniques ◽  
Flow Cytometric ◽  
Regenerated Plants ◽  
Tissue Culture Techniques ◽  
Selection Of

This is the first report of molecular markers application for the analysis of endosperm-derived callus and nonaploid kiwifruit (Actinidia chinensis var. deliciosa, formerly: Actinidia deliciosa) plants. As a source of explants, fruits of ‘Hayward’, the most popular cultivar, were used. Additionally, analyses of the nuclear DNA content and sex were conducted on the regenerated plants. Hexaploid seedlings were used as control for the flow cytometric analyses. Most of the plants (about 90%) regenerated via endosperm-derived callus possessed 2C = 9Cx DNA, which confirmed their endosperm origin and nonaploidy. Because Actinidia is a dioecious species, and female plants bearing fruits are desired by breeders, it is crucial to identify the sex of an individual at early stages of development. Analyses were conducted with ex vitro and in vitro samples. Results revealed that specific markers for a Y-chromosome applied at the callus stage allowed us to reliably predict the sex of plants regenerated from it. This is a novel application of sex-linked markers for early selection of female and male callus lines when the sex of the initial explants is still unknown, such as fresh isolated embryos and endosperm. It may have significant importance for breeding kiwifruit programs, which involve tissue culture techniques.

scholarly journals Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

2021 ◽  
Vol 27 (4) ◽  
pp. 505-515
Author(s):  
Narges Asadi ◽  
Hossein Zarei ◽  
Seyyed Hamidreza Hashemi-Petroudi ◽  
Seyyed Javad Mousavizadeh
Keyword(s):  
Genetic Variation ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Somaclonal Variation ◽  
Growth Regulators ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plantlets ◽  
Regenerated Plants ◽  
High Concentrations

Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

scholarly journals Regeneration of Tetraploid Muskmelons from Cotyledons and Their Morphological Differences from Two Diploid Muskmelon Genotypes

1992 ◽  
Vol 117 (5) ◽  
pp. 863-866 ◽  
Author(s):  
G. Fassuliotis ◽  
B.V. Nelson
Keyword(s):  
Genetic Variability ◽  
In Vitro Culture ◽  
Cucumis Melo ◽  
Morphological Changes ◽  
Guard Cells ◽  
Fruit Shape ◽  
Chromosome Counts ◽  
Regenerated Plants ◽  
Morphological Differences

`Gulfstream' and `Charentais' muskmelons (Cucumis melo. L.) plants were regenerated by in vitro culture to increase their genetic variability for resistance to root-knot nematodes (Meloidogyne spp.). While no genetic variability for root knot resistance was found, regenerated plants exhibited other traits that varied from the donor cotyledons. Chromosome counts confirmed that >75% of the somaclonal variants were tetraploid (2n = 24; 4n = 48). Tetraploids consistently exhibited micro- and macroscopic morphological changes that enabled distinction between tetraploids and diploids without chromosome counts; tetraploids contained enlarged stomates with more chloroplasts in the guard cells and pollen with a high percentage of square-appearing shapes. Tetraploids exhibited distinctive macroscopic morphological changes, including differences in leaf structure, fruit shape, blossom-end scar, number of vein tracts, and seed size.

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