scholarly journals Regeneration of Tetraploid Muskmelons from Cotyledons and Their Morphological Differences from Two Diploid Muskmelon Genotypes

1992 ◽  
Vol 117 (5) ◽  
pp. 863-866 ◽  
Author(s):  
G. Fassuliotis ◽  
B.V. Nelson
Keyword(s):  
Genetic Variability ◽  
In Vitro Culture ◽  
Cucumis Melo ◽  
Morphological Changes ◽  
Guard Cells ◽  
Fruit Shape ◽  
Chromosome Counts ◽  
Regenerated Plants ◽  
Morphological Differences

`Gulfstream' and `Charentais' muskmelons (Cucumis melo. L.) plants were regenerated by in vitro culture to increase their genetic variability for resistance to root-knot nematodes (Meloidogyne spp.). While no genetic variability for root knot resistance was found, regenerated plants exhibited other traits that varied from the donor cotyledons. Chromosome counts confirmed that >75% of the somaclonal variants were tetraploid (2n = 24; 4n = 48). Tetraploids consistently exhibited micro- and macroscopic morphological changes that enabled distinction between tetraploids and diploids without chromosome counts; tetraploids contained enlarged stomates with more chloroplasts in the guard cells and pollen with a high percentage of square-appearing shapes. Tetraploids exhibited distinctive macroscopic morphological changes, including differences in leaf structure, fruit shape, blossom-end scar, number of vein tracts, and seed size.

In vitro culture of Aloe barbadensis Mill.: quantitative DNA variations in regenerated plants

Plant Science ◽  
1993 ◽  
Vol 91 (2) ◽  
pp. 223-229 ◽  
Author(s):  
A. Cavallini ◽  
L. Natali ◽  
G. Cionini ◽  
O. Sassoli ◽  
I. Castorena-Sanchez
Keyword(s):  
In Vitro Culture ◽  
Aloe Barbadensis ◽  
Regenerated Plants ◽  
Dna Variations

scholarly journals Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture

2000 ◽  
Vol 23 (2) ◽  
pp. 479-493 ◽  
Author(s):  
Guillermo Pratta ◽  
Roxana Zorzoli ◽  
Liliana Amelia Picardi
Keyword(s):  
Multivariate Analysis ◽  
In Vitro Culture ◽  
Principal Component ◽  
Eigenvalues And Eigenvectors ◽  
Morphometric Traits ◽  
Regenerated Plants ◽  
Phenotypic Structure ◽  
Regeneration Cycle ◽  
The Stability

The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.

scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

scholarly journals Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Xiang Gan ◽  
Da Chen ◽  
Yan Deng ◽  
Jusong Yuan ◽  
Bo Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Theca Cell ◽  
Logarithmic Phase ◽  
Plateau Phase ◽  
Theca Cells ◽  
Culture Model ◽  
Theca Externa ◽  
Theca Interna ◽  
Morphological Differences

Theca cells, including theca interna cells and theca externa cells, are vital components of ovarian follicles. The aim of the present study is to identify a reliable method for the in vitro culture of theca cells from duck ovarian hierarchical (F4-F2) follicles. We improved the method for cell separation by using trypsin to further remove granular cells, and we increased the concentration of fetal bovine serum used in in vitro culture to improve cytoactivity. Cell antibody immunofluorescence (IF) showed that all inoculated cells could be stained by the CYP17A1/19A1 antibody but not by the FSHR antibody, which could stain granulosa cells. Furthermore, morphological differences were observed between the outlines of theca interna and externa cells and in their nuclei. Growth curve and CYP17A1/19A1 mRNA relative expression analyses suggested that the growth profile of theca interna cells may have been significantly different from that of theca externa cells in vitro. Theca interna cells experienced the logarithmic phase on d1–d2, the plateau phase on d2–d3, and the senescence phase after d3, while theca externa cells experienced the logarithmic phase on d1–d3, the plateau phase on d3–d5, and the senescence phase after d5. Taken together, these results suggested that we have successfully established a reliable theca cell culture model and further defined theca cell characteristics in vitro.

scholarly journals Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 154-155
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

scholarly journals Regeneration of Cucumis melo L. plants from cell suspension derived from hypocotyls callus

2013 ◽  
Vol 7 (3) ◽  
pp. 23-28
Author(s):  
Jamella Hazza Rasheed
Keyword(s):  
In Vitro Culture ◽  
Cell Suspension ◽  
Callus Culture ◽  
Cucumis Melo ◽  
Ms Medium ◽  
Regeneration Capability ◽  
Cucumis Melo L

This study aimed to investigate the regeneration capability of cell suspension derived from hypocotyl callus of the vegetable plant, muskmelon, Cucumis melo L. Culture of the densities (7.5, 9.8, 13.0, 11.2, 7.8, 4.2 )× 102 cell/ml produced callus primordia which formed typical callus culture successfully. This green – yellowish color and semi-compact callus regenerated shoots on agar solidified MS medium supplemented with 2.0mgL-1BA. These regenerates rooted readily in MS0 medium. They were adapted and routinely transferred to soil. The conclusion of this work that muskmelon plants have a good response to in vitro culture with no need to specific requirements.

scholarly journals Diallel Analysis of In Vitro Culture Traits in the Genus Lycopersicon

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 110-112 ◽  
Author(s):  
Guillermo Pratta ◽  
Lilians N. Cánepa ◽  
Roxana Zorzoli ◽  
Liliana A. Picardi
Keyword(s):  
Genetic Variability ◽  
In Vitro Culture ◽  
Opposite Effect ◽  
Diallel Analysis ◽  
Shoot Formation ◽  
Gene Effects ◽  
Callus Production ◽  
Additive Gene ◽  
Productivity Rate

Estimates of genetic variability for in vitro culture traits among the genus Lycopersicon and evaluation of the gene effects involved in callus production and shoot formation were achieved. Five parents including wild and cultivated tomato genotypes and their nonreciprocal 10 possible hybrid combinations were assayed. The callus percentage (C = number of cultures that only produced callus× 100/total number of cultures), the regeneration percentage (R = number of cultures that differentiated into shoots or primordia × 100/total number of cultures) and the productivity rate (PR = total number of shoots/total number of cultures) of each genotype were calculated 45 days after culture initiation. Diallel analysis revealed genetic variability for in vitro culture response. Wild genotypes contributed to a reduction in callus production and an increase in shoot formation while the cultivated genotypes either had an opposite effect or did not modify the expression of culture traits. Hybrids had the lowest callus production and highest shoot formation percentage. Additive gene effects were mainly involved in the expression of C and R, while both additive and nonadditive gene effects were involved in expression of PR.

scholarly journals INDUKSI DAN REGENERASI KALUS KELADI TIKUS (Typonium flagelliforme. Lodd. ) SECARA IN VITRO

2020 ◽  
Vol 13 (4) ◽  
pp. 142 ◽  
Author(s):  
SITTI FATIMAH SYAHID ◽  
NATALINI NOVA KRISTINA
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Carbon Source ◽  
Genetic Variability ◽  
In Vitro Culture ◽  
Basic Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Group B

<p>ABSTRAK<br />Keladi tikus umumnya diperbanyak secara vegetatif sehingga ragam<br />genetiknya sempit. Penelitian peningkatan keragaman genetik pada keladi<br />tikus melalui kultur in vitro telah dilakukan di Laboratorium Kultur<br />Jaringan, Balai Penelitian Tanaman Obat dan Aromatik (Balittro) Bogor<br />pada bulan April sampai Desember 2005. Bahan tanaman yang digunakan<br />adalah daun steril keladi tikus in vitro. Media dasar yang digunakan adalah<br />Murashige and Skoog (MS) yang diperkaya vitamin dari group B. Sebagai<br />sumber energi digunakan sukrosa sebanyak 30 g/l. Penelitian terdiri dari<br />dua tahap yaitu induksi dan regenerasi kalus. Perlakuan yang diuji pada<br />tahap I adalah beberapa taraf konsentrasi auksin (2,4-D) secara tunggal<br />maupun kombinasi dengan sitokinin (kinetin) terhadap induksi kalus yaitu<br />: 2,4-D 0,1 mg/l; 2,4-D 0,5 mg/l; 2,4-D 1,0 mg/l; 2,4-D 0,1 + kinetin 0,1<br />mg/l; 2,4-D 0,5 mg/l + kinetin 0,1 mg/l; 2,4-D 1.0 mg/l + kinetin 0,1 mg/l;<br />2,4-D 0,1 mg/l + kinetin 0,3 mg/l; 2,4-D 0,5 mg/l +kinetin 0,3 mg/l dan<br />2,4-D 1,0 mg/l + kinetin 0,3 mg/l. Tahap II adalah beberapa taraf<br />konsentrasi benzyl adenin untuk regenerasi kalus. Penelitian disusun<br />menggunakan rancangan acak lengkap dengan pola faktorial dan lima<br />ulangan, dan setiap ulangan terdiri dari satu eksplan. Faktor pertama<br />adalah asal kalus dan faktor kedua adalah beberapa taraf konsentrasi BA<br />yaitu : BA 0,1 mg/l ; BA 0,3 mg/l dan BA 0,5 mg/l. Parameter yang<br />diamati adalah waktu inisiasi kalus, struktur dan warna kalus, jumlah<br />tunas serta penampilan kultur secara visual. Hasil penelitian menunjukkan<br />bahwa kalus asal eksplan daun dapat diinduksi pada perlakuan 2,4-D 1,0<br />mg/l + kinetin 0,1 mg/l dan 2,4-D 1,0 mg/l + kinetin 0,3 mg/l dengan<br />waktu inisiasi 8 sampai 10 minggu setelah perlakuan. Regenerasi kalus<br />terbaik diperoleh pada medium 2,4-D 1,0 mg/l + kinetin 0,3 mg/l<br />mengandung BA 0,3 mg/l dengan rata-rata tunas dan daun yang dihasilkan<br />sebanyak 13,2 tunas dan 4,4 daun.<br />Kata kunci : Keladi tikus, Typonium flagelliforme Lodd., induksi,<br />regenerasi kalus, in vitro</p><p><br />ABSTRACT<br />Induction and regeneration of Rodent tuber calli through<br />in vitro culture<br />Rodent tuber plant (Typonium flagelliforme Lodd) is commonly<br />propagated vegetatively, the repro its genetic variation is narrow. A<br />research to increase the genetic variability of the plant was conducted in<br />Tissue Culture Laboratory of the Indonesian Medicinal and Aromatic<br />Research Institute, Bogor from April to December 2005. The leaf of<br />Rodent tuber in vitro used as an explants. Murashige and Skoog (MS)<br />medium used as basic medium, supplemented with vitamin from B group,<br />sucrose 30 g/l was added into the medium as carbon source. The research<br />consist of two steps : 1) calli induction and 2) calli regeneration. The<br />treatment tested in first step : 2.4-D 0.1 mg/l; 2.4-D 0.5 mg/l; 2.4-D 1,0<br />mg/l; 2.4-D 0.1 + kinetin 0.1 mg/l; 2.4-D 0.5 mg/l + kinetin 0.1 mg/l; 2.4-<br />D 1.0 mg/l + kinetin 0,1 mg/l; 2.4-D 0.1 mg/l + kinetin 0.3 mg/l; 2.4-D 0.5<br />mg/l + kinetin 0.3 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l. In the<br />second steps, several concentration of BA were tested i.e: BA 0,1 mg/l ;<br />BA 0,3 mg/l and BA 0,5 mg/l. The experiment was arranged in<br />completely randomized design with factorial pattern. Each treatment<br />consist of five replications. The parameters observed were time of calli<br />initiation, texture, colour of calli and number of shoot and leaves in<br />regeneration. The result showed that calli can be induced on 2.4-D 1.0<br />mg/l + kinetin 0.1 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l, eight to ten<br />weeks after culture. The best medium for shoots regeneration contains 2.4-<br />D 1.0 mg/l + kinetin 0.3 mg/l with 0.3 mg/l BA, with mean result of 13.2<br />shoots and 4.4 leaves.<br />Key words : Rodent tuber, Typonium flagelliforme Lodd. bl , induction,<br />regeneration, calli, in vitro</p>

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