scholarly journals Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture

2000 ◽  
Vol 23 (2) ◽  
pp. 479-493 ◽  
Author(s):  
Guillermo Pratta ◽  
Roxana Zorzoli ◽  
Liliana Amelia Picardi
Keyword(s):  
Multivariate Analysis ◽  
In Vitro Culture ◽  
Principal Component ◽  
Eigenvalues And Eigenvectors ◽  
Morphometric Traits ◽  
Regenerated Plants ◽  
Phenotypic Structure ◽  
Regeneration Cycle ◽  
The Stability

The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.

In vitro culture of Aloe barbadensis Mill.: quantitative DNA variations in regenerated plants

Plant Science ◽  
1993 ◽  
Vol 91 (2) ◽  
pp. 223-229 ◽  
Author(s):  
A. Cavallini ◽  
L. Natali ◽  
G. Cionini ◽  
O. Sassoli ◽  
I. Castorena-Sanchez
Keyword(s):  
In Vitro Culture ◽  
Aloe Barbadensis ◽  
Regenerated Plants ◽  
Dna Variations

scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

scholarly journals Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 154-155
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

scholarly journals 574 PB 047 STABILITY OF A VARIANT LEAF TYPE OF `THORNLESS EVERGREEN' BLACKBERRY FOLLOWING A REGENERATION CYCLE IN VITRO

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514a-514
Author(s):  
Margaret A. Norton ◽  
Robert M. Skirvin
Keyword(s):  
Somaclonal Variation ◽  
Growth Habit ◽  
Epidermal Cells ◽  
Leaf Type ◽  
Rubus Laciniatus ◽  
Regeneration Cycle ◽  
The Stability

Rubus laciniatus Willd. `Thornless Evergreen' (TE) is a chimeral blackberry with a thornless epidermis that overlies a genetically thorny interior. Most canes of TE produce leaves with 5 finely cut (lacinate) leaflets. Occasionally, canes appear which produce leaves with entire leaflets. Genetically pure thornless plants were regenerated from epidermal cells of chimeral TE with lacinate leaves. These regenerants exhibited somaclonal variation for growth habit, degree of thornlessness, and fruitfulness. All had lacinate leaves. When moved to the field, some of these regenerants produced canes with entire leaflets. To examine the stability of the entire leaflet characteristic, plants were regenerated from lacinate leaves and entire leaves of both dwarf and normal pure thornless TE regenerants. Regenerants were rooted, moved to soil, and grown in the greenhouse for observation. Stability of this characteristic will be discussed.

Hepatocytes in Primary Culture: An Alternative to LD50 Testing? Validation of a Predictive Model by Multivariate Analysis

1992 ◽  
Vol 20 (1) ◽  
pp. 8-26
Author(s):  
Anne-Françoise Peloux ◽  
Christian Fédérici ◽  
Nicole Bichet ◽  
Daniel Gouy ◽  
Jean-Paul Cano
Keyword(s):  
Multivariate Analysis ◽  
Confidence Interval ◽  
Primary Cultures ◽  
Principal Component ◽  
Neutral Red ◽  
Total Protein Content ◽  
Lactate Dehydrogenase Release ◽  
Ic50 Values

The cytotoxicity of 30 chemicals was assessed in rat hepatocyte primary cultures using four methods: lactate dehydrogenase release, neutral red uptake, the MTT assay, and measurement of total protein content. Comparison of the data obtained in vitro (IC50 values) and in vivo (LD50 values) resulted in a significant correlation (p<0.001) between IC50 values and intravenous LD50 values. The validity, as well as the predictability of the model, were determined by multivariate analysis (principal component analysis and correspondence analysis). The predictability area, expressed in IC50 values, was in the range of 0–l,500μg/ml and reached 95%, with a 75–100% confidence interval (p = 0.05). Assessment of the cytotoxicity of 54 additional chemicals would provide a more accurate predictability limit around l,500μg/ml and the estimated predictability confidence interval could be reduced to 90–100%.

scholarly journals Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

2021 ◽  
Vol 27 (4) ◽  
pp. 505-515
Author(s):  
Narges Asadi ◽  
Hossein Zarei ◽  
Seyyed Hamidreza Hashemi-Petroudi ◽  
Seyyed Javad Mousavizadeh
Keyword(s):  
Genetic Variation ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Somaclonal Variation ◽  
Growth Regulators ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plantlets ◽  
Regenerated Plants ◽  
High Concentrations

Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

scholarly journals Microevolution of a Standard Strain ofCryptococcus neoformans Resulting in Differences in Virulence and Other Phenotypes

1998 ◽  
Vol 66 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Sarah P. Franzot ◽  
Jean Mukherjee ◽  
Robert Cherniak ◽  
Lin-Chi Chen ◽  
Junia S. Hamdan ◽  
...  
Keyword(s):  
Growth Rate ◽  
In Vitro Culture ◽  
Capsular Polysaccharide ◽  
Dna Typing ◽  
Carbon Assimilation ◽  
Drug Susceptibility ◽  
Proteinase Production ◽  
The Stability ◽  
Capsule Size

ABSTRACT Cryptococcus neoformans is a major fungal pathogen for patients with debilitated immune systems. However, no information is available on the stability of virulence or of phenotypes associated with virulence for C. neoformans laboratory strains. A serendipitous observation in our laboratory that one isolate of C. neoformans ATCC 24067 (strain 52D) became attenuated after continuous in vitro culture prompted us to perform a comparative study of nine strain 24067 isolates obtained from six different research laboratories. Each isolate was characterized by DNA typing, virulence for mice, proteinase production, extracellular protein synthesis, melanin synthesis, carbon assimilation pattern, antifungal drug susceptibility, colony morphology, growth rate, agglutination titers, phagocytosis by murine macrophages, capsule size, and capsular polysaccharide structure. All isolates had similar DNA typing patterns consistent with their assignment to the same strain, although minor chromosome size polymorphisms were observed in the electrophoretic karyotypes of two isolates. Several isolates had major differences in phenotypes that may be associated with virulence, including growth rate, capsule size, proteinase production, and melanization. These findings imply that C. neoformans is able to undergo rapid changes in vitro, probably as a result of adaptation to laboratory conditions, and suggest the need for careful attention to storage and maintenance conditions. In summary, our results indicate thatC. neoformans (i) can become attenuated by in vitro culture and (ii) is capable of microevolution in vitro with the emergence of variants exhibiting new genotypic and phenotypic characteristics.

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