Influence of neonatal treatment with the pyrethroid insecticide cypermethrin on the development of dopamine receptors in the rat kidney

Dopamine receptors in glomeruli and renal cortical tubules were characterized using radioligand binding and adenylate cyclase studies. The binding of [3H]haloperidol to glomeruli and tubules was rapid, saturable with time and ligand concentration, reversible, of high affinity, and demonstrated stereoselectivity and antagonist and agonist rank potency for binding to dopamine receptors. Analysis of kinetic data and Rosenthal plots in glomeruli revealed a single class of [3H]haloperidol binding sites with an apparent dissociation constant (Kd) of 6 nM and maximum receptor density (Bmax) of 0.42 pmol/mg protein. In tubules, at least two binding sites were noted, one with an apparent Kd of 38 nM and Bmax of 1.90 pmol/mg protein and another with an apparent Kd of 183 nM and Bmax of 3.50 pmol/mg protein. Dopamine and apomorphine increased adenylate cyclase in tubular membranes while no increases were noted in glomeruli. These studies suggest that glomeruli have D2 dopamine receptors, while renal cortical tubules contain the D1 dopamine receptor.

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Characteristics of Rat Kidney Dopamine Receptors and the Effects of Renal Denervation and Dopamine Infusion on These Receptors

Nephron ◽

10.1159/000185782 ◽

1989 ◽

Vol 53 (4) ◽

pp. 358-363 ◽

Cited By ~ 6

Author(s):

Eriko Katayama ◽

Toshio Ogura ◽

Zensuke Ota

Keyword(s):

Dopamine Receptors ◽

Renal Denervation ◽

Rat Kidney ◽

Dopamine Infusion

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The Morphology of the Rat Kidney Following Folic Acid Administration

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100067996 ◽

1970 ◽

Vol 28 ◽

pp. 200-201

Author(s):

Aline Byrnes ◽

Elsa E. Ramos ◽

Minoru Suzuki ◽

E.D. Mayfield

Keyword(s):

Folic Acid ◽

De Novo ◽

Specific Activity ◽

Rat Kidney ◽

Present Report ◽

Intracellular Localization ◽

Male Rats ◽

Renal Hypertrophy ◽

Electron Microscopic ◽

Biochemical Alterations

Renal hypertrophy was induced in 100 g male rats by the injection of 250 mg folic acid (FA) dissolved in 0.3 M NaHCO3/kg body weight (i.v.). Preliminary studies of the biochemical alterations in ribonucleic acid (RNA) metabolism of the renal tissue have been reported recently (1). They are: RNA content and concentration, orotic acid-c14 incorporation into RNA and acid soluble nucleotide pool, intracellular localization of the newly synthesized RNA, and the specific activity of enzymes of the de novo pyrimidine biosynthesis pathway. The present report describes the light and electron microscopic observations in these animals. For light microscopy, kidney slices were fixed in formalin, embedded, sectioned, and stained with H & E and PAS.

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Biological and Surface Properties of C-Type Virus Particles Produced by Novikoff Ascites Hepatoma Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079395 ◽

1977 ◽

Vol 35 ◽

pp. 388-389

Author(s):

D.C. Hixson ◽

J.C. Chan ◽

J.M. Bowen ◽

E.F. Walborg

Keyword(s):

Surface Properties ◽

Ricinus Communis ◽

Rat Kidney ◽

Leukemia Virus ◽

Viral Envelope ◽

Virus Particles ◽

Chemically Induced ◽

Sarcoma Virus ◽

Hepatocarcinoma Cells ◽

Type C

Several years ago Karasaki (1) reported the production of type C virus particles by Novikoff ascites hepatocarcinoma cells. More recently, Weinstein (2) has reported the presence of type C virus particles in cell cultures derived from transplantable and primary hepatocellular carcinomas. To date, the biological function of these virus and their significance in chemically induced hepatocarcinogenesis are unknown. The present studies were initiated to determine a possible role for type C virus particles in chemically induced hepatocarcinogenesis. This communication describes results of studies on the biological and surface properties of type C virus associated with Novikoff hepatocarcinoma cells.Ecotropic and xenotropic murine leukemia virus (MuLV) activity in ascitic fluid of Novikoff tumor-bearing rats was assayed in murine sarcoma virus transformed S+L- mouse cells and S+L- mink cells, respectively. The presence of sarcoma virus activity was assayed in non-virus-producing normal rat kidney (NRK) cells. Ferritin conjugates of concanavalin A (Fer-Con wheat germ agglutinin (Fer-WGA), and Ricinus communis agglutinins I and II (Fer-RCAI and Fer-RCAII) were used to probe the structure and topography of saccharide determinants present on the viral envelope.

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The Fine Structure of Rat Renal Microbodies and Cytoplasmic Bodies Following Perfusion Fixation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073544 ◽

1973 ◽

Vol 31 ◽

pp. 620-621

Author(s):

J. M. Barrett ◽

P. M. Heidger

Keyword(s):

Fine Structure ◽

Proximal Tubule ◽

Rat Kidney ◽

Renal Proximal Tubule ◽

Convincing Evidence ◽

Cytoplasmic Bodies ◽

Rat Renal Proximal Tubule ◽

Renal Proximal Tubule Cells ◽

Perfusion Fixation

Microbodies have received extensive morphological and cytochemical investigation since they were first described by Rhodin in 1954. To our knowledge, however, all investigations of microbodies and cytoplasmic bodies of rat renal proximal tubule cells have employed immersion fixation. Tisher, et al. have shown convincing evidence of fine structural alteration of microbodies in rhesus monkey kidney following immersion fixation; these alterations were not encountered when in vivo intravascular perfusion was employed. In view of these studies, and the fact that techniques for perfusion fixation have been established specifically for the rat kidney by Maunsbach, it seemed desirable to employ perfusion fixation to study the fine structure and distribution of microbodies and cytoplasmic bodies within the rat renal proximal tubule.

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Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156547 ◽

1989 ◽

Vol 47 ◽

pp. 912-913

Author(s):

S.K. Aggarwal

Keyword(s):

Alkaline Phosphatase ◽

Rat Kidney ◽

Light And Electron Microscopy ◽

Kidney Tissue ◽

Membrane Glycoproteins ◽

Electron Microscopic ◽

Before And After ◽

Interstrand Cross Links ◽

Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

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Remission of Recurrent Cochlear Hydrops Associated With Bromocriptine Treatment for Macroprolactinoma

American Journal of Audiology ◽

10.1044/2019_aja-18-0191 ◽

2019 ◽

Vol 28 (3) ◽

pp. 548-552

Author(s):

Andro Košec ◽

Ivan Kruljac ◽

Jakov Ajduk

Keyword(s):

Dopamine Receptors ◽

Male Patient ◽

Endolymphatic Hydrops ◽

Young Male ◽

Oral Steroid ◽

Case Description ◽

Diuretic Treatment ◽

Potential Therapeutic Role ◽

Transient Improvement ◽

Systemic Glucocorticoids

Objective Current recommendations for cochlear hydrops treatment include systemic glucocorticoids and diuretics. Cochlear cells express dopamine receptors, although their role is unknown in the pathophysiology of cochlear hydrops. Case Description We report the case of remission of recurrent right-sided cochlear hydrops in a young male patient treated with bromocriptine due to pituitary macroprolactinoma. Transient improvement was observed after oral steroid and diuretic treatment, but cochlear hydrops recurred until the dose of bromocriptine was increased to 10 mg daily. Conclusion Bromocriptine may stimulate dopamine receptors in cochlear cells with potential therapeutic role in patients with cochlear hydrops. There are no widely accepted and effective treatments for endolymphatic hydrops, and identifying potential new and efficacious therapeutics is of high relevance.

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