Ascorbic Acid and Collagen Fiber Formation

1961 ◽  
pp. 89-120 ◽  
Author(s):  
Bernard S. Gould
Keyword(s):  
Ascorbic Acid ◽  
Collagen Fiber ◽  
Fiber Formation

scholarly journals Transfer of ascorbic acid across the vascular endothelium: mechanism and self-regulation

2009 ◽  
Vol 297 (1) ◽  
pp. C169-C178 ◽  
Author(s):  
James M. May ◽  
Zhi-chao Qu ◽  
Huan Qiao
Keyword(s):  
Ascorbic Acid ◽  
Barrier Function ◽  
Self Regulation ◽  
Plasma Concentrations ◽  
Anion Channel ◽  
Fiber Formation ◽  
Endothelial Barrier ◽  
Channel Blockers ◽  
Actin Stress Fiber

To determine how ascorbic acid moves from the bloodstream into tissues, we assessed transfer of the vitamin across the barrier generated by EA.hy926 endothelial cells when these were cultured on semipermeable filter supports. Ascorbate transfer from the luminal to the abluminal compartment was time dependent, inhibited by anion channel blockers and by activation of protein kinase A, but was increased by thrombin. Ascorbate transfer occurred by a paracellular route, since it did not correlate with intracellular ascorbate contents and was not rectified or saturable. Nonetheless, intracellular ascorbate inhibited the transfer of both ascorbate and radiolabeled inulin across the endothelial barrier. The increase in barrier function due to ascorbate was dependent on its intracellular concentration, significant by 15 min of incubation, prevented by the cytoskeletal inhibitor colchicine, associated with F-actin stress fiber formation, and not due to collagen deposition. These results show that ascorbate traverses the endothelial barrier by a paracellular route that is regulated by cell metabolism, ion channels, and ascorbate itself. Since the latter effect occurred over the physiological range of ascorbate plasma concentrations, it could reflect a role for the vitamin in control of endothelial barrier function in vivo.

scholarly journals Tissue Response after Subcutaneous Implantation of Different Glass Ionomer-Based Cements

2019 ◽  
Vol 30 (6) ◽  
pp. 599-606
Author(s):  
Carolina Maschietto Pucinelli ◽  
Raquel Assed Bezerra da Silva ◽  
Luã Lopes Borges ◽  
Alberto Tadeu do Nascimento Borges ◽  
Paulo Nelson-Filho ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Control Group ◽  
Advanced Stage ◽  
Glass Ionomer ◽  
Granulomatous Tissue ◽  
Subcutaneous Connective Tissue

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice after implantation of different glass ionomer-based cements (EQUIA® Forte Fil, EQUIA® Fil and Ketac™ Universal Aplicap™). Eighty-seven isogenic BALB/c mice were allocated in 12 groups, 9 were considered as experimental groups (Ketac, E. Fil and E. Forte at 7, 21 and 63 days) and 3 controls (empty polyethylene tubes at 7, 21 and 63 days). After the experimental periods, the subcutaneous connective tissue surrounding the implanted material was removed and subjected to histotechnical processing and staining with hematoxylin and eosin. A histopathological description of the tissue reaction surrounding each material and a semi-quantitative analysis of collagen fiber formation and inflammatory infiltrate were performed. Additionally, the thickness of the granulomatous tissue in contact with each material was measured. Data were analyzed statistically (α=0.05) by the Kruskal-Wallis test, followed by Dunn post-test. Initially, the collagen fiber formation was not different among all the tested materials (p>0.05) but was different at 21 days with the control group presenting the most advanced stage of collagen fiber formation. At 63 days, EQUIA® Forte Fil group showed the most advanced stage of collagen fiber formation, compared to EQUIA® Fil group (p<0.05). The inflammatory infiltrate was not different among the tested materials in any experimental period (p>0.05). The thickness of the granulomatous tissue was greater in the E. Forte group, compared to control in all periods. All glass ionomer-based cements showed tissue compatibility, according to the evaluated parameters.

scholarly journals Inhibitory Effect of Heparin on Collagen Fiber Formation in Hepatic Cells in Culture.

1997 ◽  
Vol 22 (5) ◽  
pp. 533-538 ◽  
Author(s):  
Kazumi Akimoto ◽  
Masashi Ikeda ◽  
Kenji Sorimachi
Keyword(s):  
Collagen Fiber ◽  
Inhibitory Effect ◽  
Fiber Formation ◽  
Cells In Culture ◽  
Hepatic Cells

scholarly journals Prophylactic and therapeutic treatment with the flavonone sakuranetin ameliorates LPS-induced acute lung injury

2017 ◽  
Vol 312 (2) ◽  
pp. L217-L230 ◽  
Author(s):  
Márcia Isabel Bittencourt-Mernak ◽  
Nathalia M. Pinheiro ◽  
Fernanda P. R. Santana ◽  
Marina P. Guerreiro ◽  
Beatriz M. Saraiva-Romanholo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Function ◽  
Lung Injury ◽  
Collagen Fiber ◽  
Pulmonary Inflammation ◽  
Fiber Formation ◽  
Therapeutic Effects ◽  
Therapeutic Treatment ◽  
Fiber Deposition

Sakuranetin is the main isolate flavonoid from Baccharis retusa ( Asteraceae) leaves and exhibits anti-inflammatory and antioxidative activities. Acute respiratory distress syndrome is an acute failure of the respiratory system for which effective treatment is urgently necessary. This study investigated the preventive and therapeutic effects of sakuranetin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Animals were treated with intranasal sakuranetin 30 min before or 6 h after instillation of LPS. Twenty-four hours after ALI was induced, lung function, inflammation, macrophages population markers, collagen fiber deposition, the extent of oxidative stress, and the expression of matrix metalloprotease-9 (MMP-9), tissue inhibitor of MMP-9 (TIMP-1) and NF-κB were evaluated. The animals began to show lung alterations 6 h after LPS instillation, and these changes persisted until 24 h after LPS administration. Preventive and therapeutic treatment with sakuranetin reduced the neutrophils in the peripheral blood and in the bronchial alveolar lavage. Sakuranetin treatment also reduced macrophage populations, particularly that of M1-like macrophages. In addition, sakurnaetin treatment reduced keratinocyte-derived chemokines (IL-8 homolog) and NF-κB levels, collagen fiber formation, MMM-9 and TIMP-1-positive cells, and oxidative stress in lung tissues compared with LPS animals treated with vehicle. Finally, sakuranetin treatment also reduced total protein, and the levels of TNF-α and IL-1β in the lung. This study shows that sakuranetin prevented and reduced pulmonary inflammation induced by LPS. Because sakuranetin modulates oxidative stress, the NF-κB pathway, and lung function, it may constitute a novel therapeutic candidate to prevent and treat ALI.

Collagen fiber formation and alkaline phosphatase

1951 ◽  
Vol 33 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Norman I. Gold ◽  
Bernard S. Gould
Keyword(s):  
Alkaline Phosphatase ◽  
Collagen Fiber ◽  
Fiber Formation

scholarly journals Effects of Recombinant Angiogenin on Collagen Fiber Formation and Angiogenesis in the Dermis of Wistar Rats

2021 ◽  
Vol Volume 14 ◽  
pp. 187-196
Author(s):  
Natalia V Yurina ◽  
Tatiana A Ageeva ◽  
Aleksandr M Goryachkin ◽  
Nikolay A Varaksin ◽  
Tatiana G Ryabicheva ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Collagen Fiber ◽  
Fiber Formation

Production of a Novel, Self-Assembled, Collagenous Matrix Mimicking the Hierarchical Structure of Native Aligned Connective Tissue

1995 ◽  
Vol 414 ◽  
Author(s):  
G. D. Pins ◽  
D. L. Christiansen ◽  
R. Patel ◽  
F. H. Silver
Keyword(s):  
Self Assembly ◽  
Collagen Fiber ◽  
Type I Collagen ◽  
Tensile Tests ◽  
Fiber Formation ◽  
Morphological Properties ◽  
Uniaxial Tensile ◽  
Type I ◽  
Native Collagen

AbstractThe primary goal of the biomaterials scientist and tissue engineer is to create a biocompatible implant which mimics the mechanical and morphological properties of the tissue being replaced. In vitro experimentation has documented the propensity of soluble type I collagen to self-assemble and form microscopic collagen fibrils with periodic banding analogous to native collagen fiber. Our laboratory has further investigated in vitro self-assembly by incorporating several of the “natural” processes into a multi-step fiber formation procedure which generates macroscopic collagen fiber from its molecular constituents. Results of uniaxial tensile tests and ultrastructural analyses indicate that these coextruded and stretched collagen fibers have mechanical properties and fibrillar substructure comparable to that observed in native collagen fiber.

scholarly journals Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2646
Author(s):  
Alexes C. Daquinag ◽  
Zhanguo Gao ◽  
Cale Fussell ◽  
Kai Sun ◽  
Mikhail G. Kolonin
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Collagen Fiber ◽  
Normal Function ◽  
Fiber Formation ◽  
Amino Terminus ◽  
Matrix Rigidity ◽  
Collagen Binding ◽  
Adipose Stromal Cells

Proper processing of collagens COL1 and COL6 is required for normal function of adipose tissue and skeletal muscle. Proteoglycan decorin (DCN) regulates collagen fiber formation. The amino-terminus of DCN is modified with an O-linked glycosaminoglycan (GAG), the function of which has remained unclear. Previously, non-glycanated DCN (ngDCN) was identified as a marker of adipose stromal cells. Here, we identify MMP14 as the metalloprotease that cleaves DCN to generate ngDCN. We demonstrate that mice ubiquitously lacking DCN GAG (ngDCN mice) have reduced matrix rigidity, enlarged adipocytes, fragile skin, as well as skeletal muscle hypotrophy, fibrosis, and dysfunction. Our results indicate that DCN deglycanation results in reduced intracellular DCN—collagen binding and increased production of truncated COL6 chains, leading to aberrant procollagen processing and extracellular localization. This study reveals that the GAG of DCN functions to regulate collagen assembly in adipose tissue and skeletal muscle and uncovers a new mechanism of matrix dysfunction in obesity and aging.

scholarly journals Subcutaneous connective tissue response to primary root canal filling materials

2011 ◽  
Vol 22 (3) ◽  
pp. 203-211 ◽  
Author(s):  
Alexandra Mussolino de Queiroz ◽  
Sada Assed ◽  
Alberto Consolaro ◽  
Paulo Nelson-Filho ◽  
Mario Roberto Leonardo ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Quantitative Analysis ◽  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Filling Materials ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue

This study evaluated the response of the subcutaneous connective tissue of BALB/c mice to root filling materials indicated for primary teeth: zinc oxide/eugenol cement (ZOE), Calen paste thickened with zinc oxide (Calen/ZO) and Sealapex sealer. The mice (n=102) received polyethylene tube implants with the materials, thereby forming 11 groups, as follows: I, II, III: Calen/ZO for 7, 21 and 63 days, respectively; IV, V, VI: Sealapex for 7, 21 and 63 days, respectively; VII, VIII, IX: ZOE for 7, 21 and 63 days, respectively; X and XI: empty tube for 7 and 21 days, respectively. The biopsied tissues were submitted to histological analysis (descriptive analysis and semi-quantitative analysis using a scoring system for collagen fiber formation, tissue thickness and inflammatory infiltrate). A quantitative analysis was performed by measuring the area and thickness of the granulomatous reactionary tissue (GRT). Data were analyzed by Kruskal-Wallis, ANOVA and Tukey's post-hoc tests (?=0.05). There was no significant difference (p>0.05) among the materials with respect to collagen fiber formation or GRT thickness. However, Calen/ZO produced the least severe inflammatory infiltrate (p<0.05). The area of the GRT was significantly smaller (p<0.05) for Calen/ZO and Sealapex. In conclusion, Calen/ZO presented the best tissue reaction, followed by Sealapex and ZOE.

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