Secretory IgA differentially promotes adherence of type 1-piliated Escherichia coli to immortalized vaginal epithelial cell lines

Urology ◽  
2001 ◽  
Vol 57 (3) ◽  
pp. 556-561 ◽  
Author(s):  
Richard A Schoor ◽  
Byron Anderson ◽  
David J Klumpp ◽  
Anthony J Schaeffer
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Secretory Iga ◽  
Vaginal Epithelial Cell ◽  
Epithelial Cell Lines

scholarly journals CD14- and Toll-Like Receptor-Dependent Activation of Bladder Epithelial Cells by Lipopolysaccharide and Type 1 Piliated Escherichia coli

2003 ◽  
Vol 71 (3) ◽  
pp. 1470-1480 ◽  
Author(s):  
Joel D. Schilling ◽  
Steven M. Martin ◽  
David A. Hunstad ◽  
Kunal P. Patel ◽  
Matthew A. Mulvey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
E Coli ◽  
Epithelial Cell Lines ◽  
Renal Epithelial Cell Line

ABSTRACT The gram-negative bacterium Escherichia coli is the leading cause of urinary tract infection. The interaction between type 1 piliated E. coli and bladder epithelial cells leads to the rapid production of inflammatory mediators, such as interleukin-6 (IL-6) and IL-8. Conflicting reports have been published in the literature regarding the mechanism by which uroepithelial cells are activated by type 1 piliated E. coli. In particular, the role of lipopolysaccharide (LPS) in these responses has been an area of significant debate. Much of the data arguing against LPS-mediated activation of bladder epithelial cells have come from studies using a renal epithelial cell line as an in vitro model of the urinary epithelium. In this report, we analyzed three bladder epithelial cell lines and demonstrated that they all respond to LPS. Furthermore, the LPS responsivity of the cell lines directly correlated with their ability to generate IL-6 after E. coli stimulation. The LPS receptor complex utilized by the bladder epithelial cell lines included CD14 and Toll-like receptors, and signaling involved the activation of NF-κB and p38 mitogen-activated protein kinase. Also, reverse transcription-PCR analysis demonstrated that bladder epithelial cells express CD14 mRNA. Thus, the molecular machinery utilized by bladder epithelial cells for the recognition of E. coli is very similar to that described for traditional innate immune cells, such as macrophages. In contrast, the A498 renal epithelial cell line did not express CD14, was hyporesponsive to LPS stimulation, and demonstrated poor IL-6 responses to E. coli.

M1810 Interferon Gamma-Induced STAT1 Phosphorylation is Blocked by Adherent-Invasive Escherichia coli in Human Intestine Epithelial Cell Lines

2010 ◽  
Vol 138 (5) ◽  
pp. S-424
Author(s):  
Juan C. Ossa ◽  
Nathan Ho ◽  
Eytan Wine ◽  
Nelly Leung ◽  
Scott D. Gray-Owen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Interferon Gamma ◽  
Human Intestine ◽  
Epithelial Cell Lines

scholarly journals Oral and Vaginal Epithelial Cell Lines Bind and Transfer Cell-Free Infectious HIV-1 to Permissive Cells but Are Not Productively Infected

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e98077 ◽  
Author(s):  
Arinder Kohli ◽  
Ayesha Islam ◽  
David L. Moyes ◽  
Celia Murciano ◽  
Chengguo Shen ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Transfer Cell ◽  
Vaginal Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Hiv 1

scholarly journals Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines

Gut Pathogens ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 31 ◽  
Author(s):  
Ulrike Frömmel ◽  
Alexander Böhm ◽  
Jörg Nitschke ◽  
Jörg Weinreich ◽  
Julia Groß ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Wild Animals ◽  
Pathogenic Escherichia Coli ◽  
Epithelial Cell Lines

scholarly journals Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

2000 ◽  
Vol 68 (12) ◽  
pp. 6744-6749 ◽  
Author(s):  
Hany Sahly ◽  
Rainer Podschun ◽  
Tobias A. Oelschlaeger ◽  
Michael Greiwe ◽  
Haralambos Parolis ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Parent Strain ◽  
Hemagglutinating Activity ◽  
Epithelial Cell Line ◽  
Epithelial Cell Lines ◽  
Bladder Cells ◽  
Type 3 ◽  
Capsular Material

ABSTRACT The adhesion of K21a, K26, K36, and K50 capsulatedKlebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistantKlebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization ofKlebsiella.

scholarly journals Correction: Oral and Vaginal Epithelial Cell Lines Bind and Transfer Cell-Free Infectious HIV-1 to Permissive Cells but Are Not Productively Infected

PLoS ONE ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. e0229553
Author(s):  
Arinder Kohli ◽  
Ayesha Islam ◽  
David L. Moyes ◽  
Celia Murciano ◽  
Chengguo Shen ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Transfer Cell ◽  
Vaginal Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Hiv 1
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