Relationship between clinical symptoms and enzyme-producing bacteria isolated from infected root canals

1994 ◽  
Vol 20 (2) ◽  
pp. 75-77 ◽  
Author(s):  
Kazuko Hashioka ◽  
Kazuyoshi Suzuki ◽  
Tsutomu Yoshida ◽  
Akinobu Nakane ◽  
Naoki Horiba ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Root Canals ◽  
Infected Root

Correlations between endotoxin and clinical symptoms or radiolucent areas in infected root canals

1991 ◽  
Vol 71 (4) ◽  
pp. 492-495 ◽  
Author(s):  
Naoki Horiba ◽  
Yoshinori Maekawa ◽  
Yoshie Abe ◽  
Masato Ito ◽  
Toru Matsumoto ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Root Canals ◽  
Infected Root

scholarly journals Microbiological Analysis of Primary Infected Root Canals with Symptomatic and Asymptomatic Apical Periodontitis of Young Permanent Teeth

2020 ◽  
Vol 24 (3) ◽  
pp. 44-51
Author(s):  
Jelena Marinković ◽  
Tatjana Marković ◽  
Snežana Brkić ◽  
Milena Radunović ◽  
Ivan Soldatović ◽  
...  
Keyword(s):  
School Children ◽  
Clinical Symptoms ◽  
Treatment Strategies ◽  
Apical Periodontitis ◽  
Permanent Teeth ◽  
Microbiological Analysis ◽  
Microbial Composition ◽  
Root Canals ◽  
Facultative Anaerobes ◽  
Infected Root

SummaryBackground/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms.Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symtoms were recorded.Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms.Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.

The relationship between clinical symptoms and anaerobic bacteria from infected root canals

1992 ◽  
Vol 18 (11) ◽  
pp. 558-561 ◽  
Author(s):  
Kazuko Hashioka ◽  
Masahiro Yamasaki ◽  
Akinobu Nakane ◽  
Naoki Horiba ◽  
Hiroshi Nakamura
Keyword(s):  
Clinical Symptoms ◽  
Anaerobic Bacteria ◽  
Root Canals ◽  
Infected Root ◽  
The Relationship

In vitroactivity of photoactivated disinfection using a diode laser in infected root canals

2014 ◽  
Vol 72 (8) ◽  
pp. 673-680 ◽  
Author(s):  
Ulrich Schiffner ◽  
Georg Cachovan ◽  
Jochen Bastian ◽  
Anton Sculean ◽  
Sigrun Eick
Keyword(s):  
Diode Laser ◽  
Root Canals ◽  
Infected Root

scholarly journals A 16S rDNA-based nested PCR protocol to detect Campylobacter gracilis in oral infections

2003 ◽  
Vol 17 (2) ◽  
pp. 142-146 ◽  
Author(s):  
José Freitas Siqueira Júnior ◽  
Isabela das Neves Rôças
Keyword(s):  
16S Rdna ◽  
High Sensitivity ◽  
High Specificity ◽  
Cross Reactivity ◽  
Nested Polymerase Chain Reaction ◽  
Oral Infections ◽  
Root Canals ◽  
Infected Root ◽  
Pcr Products ◽  
Species Specific

The aim of this study was to describe a 16S rDNA-based nested polymerase chain reaction (nPCR) assay to investigate the occurrence of Campylobacter gracilis in oral infections. Samples were collected from ten infected root canals, ten cases of acute periradicular abscesses and eight cases of adult marginal periodontitis. DNA extracted from the samples was initially amplified using universal 16S rDNA primers. A second round of amplification used the first PCR products to detect C. gracilis using oligonucleotide primers designed from species-specific 16S rDNA signature sequences. The nPCR assay used in this study showed a detection limit of 10 C. gracilis cells and no cross-reactivity was observed with nontarget bacteria. C. gracilis was detected in the three types of oral infections investigated - 4/10 infected root canals; 2/10 acute periradicular abscesses; and 1/8 subgingival specimens from adult periodontitis. The method proposed in this study showed both high sensitivity and high specificity to directly detect C. gracilis in samples from root canal infections, abscesses, and subgingival plaque. Our findings confirmed that C. gracilis may be a member of the microbiota associated with distinct oral infections, and its specific role in such diseases requires further clarification.

scholarly journals Efficacy of Endodontic Treatment on the Persistence of Selected Endodontic Pathogens and on Radiographical Periapical Healing

2015 ◽  
Vol 17 (2) ◽  
pp. 43
Author(s):  
Rodolfo Zeledón DDS, Esp ◽  
Carolina Ballestero DDS, Esp ◽  
Erick Hernández DDS. Esp ◽  
Tatiana Ramírez DDS, MSD ◽  
Marianela Benavides DDS, Esp ◽  
...  
Keyword(s):  
Positive Impact ◽  
Fusobacterium Nucleatum ◽  
Dental Students ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Root Canals ◽  
Prevotella Nigrescens ◽  
Infected Root

The objective of this clinical study was to evaluate by polymerase chain reaction (PCR) the antimicrobial efficacy and the outcome of an endodontic protocol treatment (EPT) performed by under-graduated dental students on infected root canals associated with periapical lesions. Fifty-six patients attending for treatment of pulp necrosis and apical periodontitis were included. A specific EPT approach was performed and DNA extracts were taken at the baseline (S1), after a disinfection-neutralization procedure (S2), post-chemomechanical preparation (S3) and after Intracanal medication (S4) for the presence of specific bacteria. The outcome of the EPT was assessed radiographically using the Periapical Index-system (PAI) after 18-month follow-up. An intergroup evaluation shows that when comparing S1 to S3, S1 to S4, S2 to S3, and S2 to S4, the presence of bacteria were significantly reduced (P < 0.05). Finally, S4 was not significantly reduced when compared with S3 (P > 0.05). The most prevalent species in their respect order were Actinomyces Israelii > Enterococcus Faecalis > Fusobacterium Nucleatum/Prevotella Nigrescens > Phorphyromonas Endodontalis. After the 18-month follow-up, the overall success rate of root canal – treated teeth was of 88% (PAI 1 or 2). This In Vivo study demonstrated that EPT significantly reduced the number of cases with positive results for the studied bacteria, showing a profound positive impact in the outcome of endodontic treatment of teeth diagnosed with Pulp Necrosis and Apical Periodontitis. 

scholarly journals To Compare the Bacterial Reduction from the Infected Root Canals Using ProTaper Universal, WaveOne and NeoNiTi Instrumentation Systems

2019 ◽  
Vol 5 (2) ◽  
pp. 40
Author(s):  
Renubala Sroa ◽  
Baljeet Sidhu ◽  
Neha Mengi ◽  
Sarbjot Singh ◽  
Maheep Sidhu
Keyword(s):  
Bacterial Reduction ◽  
Root Canals ◽  
Infected Root
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