The relationship between clinical symptoms and anaerobic bacteria from infected root canals

Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification.Methods. Informed consent was obtained from all subjects (age ranges, 34–71 years). Nine infected root canals with periapical lesions from 7 subjects were included. Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing.Results. The mean bacterial count (CFU) in root canals was(0.5±1.1)×106(range8.0×101–3.1×106), and anaerobic bacteria were predominant (89.8%). The predominant isolates wereOlsenella(25.4%),Mogibacterium(17.7%),Pseudoramibacter(17.7%),Propionibacterium(11.9%) andParvimonas(5.9%).Conclusion. The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals. The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes.

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Correlations between endotoxin and clinical symptoms or radiolucent areas in infected root canals

Oral Surgery Oral Medicine Oral Pathology ◽

10.1016/0030-4220(91)90438-i ◽

1991 ◽

Vol 71 (4) ◽

pp. 492-495 ◽

Cited By ~ 52

Author(s):

Naoki Horiba ◽

Yoshinori Maekawa ◽

Yoshie Abe ◽

Masato Ito ◽

Toru Matsumoto ◽

...

Keyword(s):

Clinical Symptoms ◽

Root Canals ◽

Infected Root

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Microbiological Analysis of Primary Infected Root Canals with Symptomatic and Asymptomatic Apical Periodontitis of Young Permanent Teeth

Balkan Journal of Dental Medicine ◽

10.2478/bjdm-2020-0027 ◽

2020 ◽

Vol 24 (3) ◽

pp. 44-51

Author(s):

Jelena Marinković ◽

Tatjana Marković ◽

Snežana Brkić ◽

Milena Radunović ◽

Ivan Soldatović ◽

...

Keyword(s):

School Children ◽

Clinical Symptoms ◽

Treatment Strategies ◽

Apical Periodontitis ◽

Permanent Teeth ◽

Microbiological Analysis ◽

Microbial Composition ◽

Root Canals ◽

Facultative Anaerobes ◽

Infected Root

SummaryBackground/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms.Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symtoms were recorded.Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms.Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.

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Evaluation of antimicrobial effectiveness of two endodontic irrigation solutions on the microbial reduction of Enterococcus faecalis in infected root canals (in vitro study)

International Dental & Medical Journal of Advanced Research - VOLUME 2015 ◽

10.15713/ins.idmjar.33 ◽

2015 ◽

Vol 1 (1) ◽

pp. 1-6

Author(s):

Murtada Shahadeh ◽

Mohamad Osama Jabban ◽

Zaher Altaki

Keyword(s):

Enterococcus Faecalis ◽

In Vitro Study ◽

Microbial Reduction ◽

Vitro Study ◽

Root Canals ◽

Infected Root ◽

Endodontic Irrigation ◽

Antimicrobial Effectiveness

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Role of filter media in an anaerobic fixed-bed reactor

Water Science & Technology ◽

10.2166/wst.1995.0353 ◽

1995 ◽

Vol 31 (9) ◽

pp. 137-144 ◽

Cited By ~ 3

Author(s):

T. Miyahara ◽

M. Takano ◽

T. Noike

Keyword(s):

Anaerobic Bacteria ◽

Fixed Bed ◽

Methanogenic Bacteria ◽

The Other ◽

Fixed Bed Reactor ◽

Filter Media ◽

Fixed Bed Reactors ◽

Attached Bacteria ◽

The Relationship

The relationship between the filter media and the behaviour of anaerobic bacteria was studied using anaerobic fixed-bed reactors. At an HRT of 48 hours, the number of suspended acidogenic bacteria was higher than those attached to the filter media. On the other hand, the number of attached methanogenic bacteria was more than ten times as higher than that of suspended ones. The numbers of suspended and deposited acidogenic and methanogenic bacteria in the reactor operated at an HRT of 3 hours were almost the same as those in the reactor operated at an HRT of 48 hours. Accumulation of attached bacteria was promoted by decreasing the HRT of the reactor. The number of acidogenic bacteria in the reactor packed sparsely with the filter media was higher than that in the closely packed reactor. The number of methanogenic bacteria in the sparsely packed reactor was lower than that in the closely packed reactor.

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Decision making under ambiguity and risk in adolescent-onset schizophrenia

BMC Psychiatry ◽

10.1186/s12888-021-03230-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Dandan Li ◽

Fengyan Zhang ◽

Lu Wang ◽

Yifan Zhang ◽

Tingting Yang ◽

...

Keyword(s):

Decision Making ◽

Executive Function ◽

Iowa Gambling Task ◽

Clinical Symptoms ◽

Healthy Controls ◽

Syndrome Scale ◽

Significant Difference ◽

Abnormal Pattern ◽

Negative Syndrome ◽

The Relationship

Abstract Objective Numerous studies have identified impaired decision making (DM) under both ambiguity and risk in adult patients with schizophrenia. However, the assessment of DM in patients with adolescent-onset schizophrenia (AOS) has been challenging as a result of the instability and heterogeneity of manifestations. The Iowa Gambling Task (IGT) and Game of Dice Task (GDT), which are frequently used to evaluate DM respectively under ambiguity and risk, are sensitive to adolescents and neuropsychiatric patients. Our research intended to examine the performance of DM in a relatively large sample of patients with AOS using the above-mentioned two tasks. We also aimed to take a closer look at the relationship between DM and symptom severity of schizophrenia. Methods We compared the performance of DM in 71 patients with AOS and 53 well-matched healthy controls using IGT for DM under ambiguity and GDT for DM under risk through net scores, total scores and feedback ration. Neuropsychological tests were conducted in all participants. Clinical symptoms were evaluated by using Positive and Negative Syndrome Scale (PANSS) in 71 patients with AOS. Pearson’s correlation revealed the relationship among total score of DM and clinical and neuropsychological data. Results Compared to healthy controls, patients with AOS failed to show learning effect and had a significant difference on the 5th block in IGT and conducted more disadvantageous choices as well as exhibited worse negative feedback rate in GDT. Apart from DM impairment under risk, diminished DM abilities under ambiguity were found related to poor executive function in AOS in the present study. Conclusions Our findings unveiled the abnormal pattern of DM in AOS, mainly reflected under the risky condition, extending the knowledge on the performance of DM under ambiguity and risk in AOS. Inefficient DM under risk may account for the lagging impulse control and the combined effects of developmental disease. In addition, our study demonstrated that the performance on IGT was related to executive function in AOS.

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