scholarly journals Microbiological Analysis of Primary Infected Root Canals with Symptomatic and Asymptomatic Apical Periodontitis of Young Permanent Teeth

2020 ◽  
Vol 24 (3) ◽  
pp. 44-51
Author(s):  
Jelena Marinković ◽  
Tatjana Marković ◽  
Snežana Brkić ◽  
Milena Radunović ◽  
Ivan Soldatović ◽  
...  
Keyword(s):  
School Children ◽  
Clinical Symptoms ◽  
Treatment Strategies ◽  
Apical Periodontitis ◽  
Permanent Teeth ◽  
Microbiological Analysis ◽  
Microbial Composition ◽  
Root Canals ◽  
Facultative Anaerobes ◽  
Infected Root

SummaryBackground/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms.Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symtoms were recorded.Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms.Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.

scholarly journals Efficacy of Endodontic Treatment on the Persistence of Selected Endodontic Pathogens and on Radiographical Periapical Healing

2015 ◽  
Vol 17 (2) ◽  
pp. 43
Author(s):  
Rodolfo Zeledón DDS, Esp ◽  
Carolina Ballestero DDS, Esp ◽  
Erick Hernández DDS. Esp ◽  
Tatiana Ramírez DDS, MSD ◽  
Marianela Benavides DDS, Esp ◽  
...  
Keyword(s):  
Positive Impact ◽  
Fusobacterium Nucleatum ◽  
Dental Students ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Root Canals ◽  
Prevotella Nigrescens ◽  
Infected Root

The objective of this clinical study was to evaluate by polymerase chain reaction (PCR) the antimicrobial efficacy and the outcome of an endodontic protocol treatment (EPT) performed by under-graduated dental students on infected root canals associated with periapical lesions. Fifty-six patients attending for treatment of pulp necrosis and apical periodontitis were included. A specific EPT approach was performed and DNA extracts were taken at the baseline (S1), after a disinfection-neutralization procedure (S2), post-chemomechanical preparation (S3) and after Intracanal medication (S4) for the presence of specific bacteria. The outcome of the EPT was assessed radiographically using the Periapical Index-system (PAI) after 18-month follow-up. An intergroup evaluation shows that when comparing S1 to S3, S1 to S4, S2 to S3, and S2 to S4, the presence of bacteria were significantly reduced (P < 0.05). Finally, S4 was not significantly reduced when compared with S3 (P > 0.05). The most prevalent species in their respect order were Actinomyces Israelii > Enterococcus Faecalis > Fusobacterium Nucleatum/Prevotella Nigrescens > Phorphyromonas Endodontalis. After the 18-month follow-up, the overall success rate of root canal – treated teeth was of 88% (PAI 1 or 2). This In Vivo study demonstrated that EPT significantly reduced the number of cases with positive results for the studied bacteria, showing a profound positive impact in the outcome of endodontic treatment of teeth diagnosed with Pulp Necrosis and Apical Periodontitis. 

Relationship between clinical symptoms and enzyme-producing bacteria isolated from infected root canals

1994 ◽  
Vol 20 (2) ◽  
pp. 75-77 ◽  
Author(s):  
Kazuko Hashioka ◽  
Kazuyoshi Suzuki ◽  
Tsutomu Yoshida ◽  
Akinobu Nakane ◽  
Naoki Horiba ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Root Canals ◽  
Infected Root

scholarly journals New Insights into the Microbial Profiles of Infected Root Canals in Traumatized Teeth

2020 ◽  
Vol 9 (12) ◽  
pp. 3877
Author(s):  
Lokeshwaran Manoharan ◽  
Malin Brundin ◽  
Olena Rakhimova ◽  
Luis Chávez de Paz ◽  
Nelly Romani Vestman
Keyword(s):  
Root Canal ◽  
Permanent Teeth ◽  
Root Canals ◽  
Tooth Position ◽  
Root Canal Disinfection ◽  
Infected Root ◽  
Traumatic Dental Injuries ◽  
Molecular Sequencing ◽  
Dental Injuries ◽  
Oral Microorganisms

Traumatic dental injuries in young individuals are often exposed to the invasion of oral microorganisms that leads to pulp necrosis. Infective necrosis in permanent teeth not-fully-developed causes aberrant root formation. Regeneration endodontic treatments (RETs) have shown promising results by promoting continued root development by stem cells. Critical to the success of RET is the thorough disinfection of the pulpal space. To establish effective antimicrobial protocols for root canal disinfection, the invading microorganisms need to be identified. In the present study, we use a combination of culture-based and high-throughput molecular sequencing techniques to investigate the microbial profiles from traumatized teeth (30 cases) and controls, i.e., teeth with pulp infections not caused by trauma (32 cases). Overall, a high microbial diversity in traumatized necrotic teeth was observed. Eubacterium yurii subsps. yurii and margaretiae, as well as key ‘bridging oral species’ F. nucleatum sp., Polymorphum and Corynebacterium matruchotti, were highly associated with traumatized teeth. The microbial compositions of traumatized teeth differed considerably from those of infected teeth not caused by trauma. Age and tooth position also influence microbial compositions. In conclusion, we show that the root canal microflora of traumatized teeth is highly diverse, and it differs from root canal infections not caused by trauma.

scholarly journals Detection of Treponema Denticola in Symptomatic Apical Periodontitis and in Symptomatic Apical Abscesses by Real-Time PCR

2009 ◽  
Vol 03 (02) ◽  
pp. 107-113 ◽  
Author(s):  
Selcuk M. Ozbek ◽  
Ahmet Ozbek ◽  
Aziz S. Erdogan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Apical Periodontitis ◽  
Treponema Denticola ◽  
Root Canals ◽  
Infected Root ◽  
Carious Lesions ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Microbial Samples

ABSTRACTObjectives: The aim of this study was to investigate the presence of Treponema denticola in symptomatic apical periodontitis and in symptomatic apical abscesses by real-time polymerase chain reaction (PCR) method.Methods: Microbial samples were collected from 60 single-rooted teeth having carious lesions and necrotic pulps. For each tooth, clinical data including patient symptoms were recorded. Teeth were categorized by diagnosis as having symptomatic apical periodontitis or symptomatic apical abscess. Aseptic microbial samples were collected using paper points from 30 infected root canals and from aspirates of 30 abscesses. DNA was extracted from the samples by using a QIAamp® DNA mini-kit and analyzed with real-time PCR.Results: T. denticola was detected in 24 of 30 cases diagnosed as symptomatic apical abscesses (80%), and 19 of 30 cases diagnosed as symptomatic apical periodontitis (63.3%). In general T. denticola was found in 43 of 60 cases (71.6%).Conclusions: Our findings suggest that T. denticola can participate in the pathogenesis of symptomatic apical abscesses. (Eur J Dent 2009;3:107-113)

Correlations between endotoxin and clinical symptoms or radiolucent areas in infected root canals

1991 ◽  
Vol 71 (4) ◽  
pp. 492-495 ◽  
Author(s):  
Naoki Horiba ◽  
Yoshinori Maekawa ◽  
Yoshie Abe ◽  
Masato Ito ◽  
Toru Matsumoto ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Root Canals ◽  
Infected Root

Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth

2013 ◽  
Vol 38 (2) ◽  
pp. 155-160 ◽  
Author(s):  
N Topcuoglu ◽  
E Bozdoğan ◽  
O Aktoren ◽  
G Kulekci
Keyword(s):  
Primary Teeth ◽  
Bacterial Species ◽  
Treatment Strategies ◽  
Fusobacterium Nucleatum ◽  
Primary Tooth ◽  
Microarray Technology ◽  
Microbial Composition ◽  
Root Canals ◽  
Endodontic Infections ◽  
Clinical Conditions

Objective: Knowledge of the microbial composition of deciduous endodontic infections is limited. This study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. Study Design: The samples were collected from 30 root canals of primary teeth with primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (Parocheck®). Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequently isolated bacterium (96.7%), followed by Prevotella intermedia (86.7%), Parvimonas micra (83.3%), Treponema denticola (76.7%) and Tannerella forsythia (66.7%). These bacteria were also present in high levels. All pairs of bacterial species were positively associated (RR≯1), except P.intermedia and P.micra. On average, five species (range:3-8) were detected per amplified sample. Root canals of teeth with ≯5 different species were statistically associated with periapical radiolucency (P=0.049). Conclusions: Primary teeth with endodontic infections show a highly diverse variety of bacteria, in which the most prevalent specie are present in high proportions. The well-directed use of the improved microarray technology will provide additional valuable information for causative factors associated with endodontic diseases, helping to develop more successful antibacterial or anti-inflammatory treatment strategies.

The relationship between clinical symptoms and anaerobic bacteria from infected root canals

1992 ◽  
Vol 18 (11) ◽  
pp. 558-561 ◽  
Author(s):  
Kazuko Hashioka ◽  
Masahiro Yamasaki ◽  
Akinobu Nakane ◽  
Naoki Horiba ◽  
Hiroshi Nakamura
Keyword(s):  
Clinical Symptoms ◽  
Anaerobic Bacteria ◽  
Root Canals ◽  
Infected Root ◽  
The Relationship

Yeasts in Apical Periodontitis

2003 ◽  
Vol 14 (2) ◽  
pp. 128-137 ◽  
Author(s):  
T.M.T. Waltimo ◽  
B.H. Sen ◽  
J.H. Meurman ◽  
D. Ørstavik ◽  
M.P.P. Haapasalo
Keyword(s):  
Root Canal ◽  
Antimicrobial Agents ◽  
Root Apex ◽  
Apical Periodontitis ◽  
Dentinal Tubules ◽  
Root Canals ◽  
Infected Root ◽  
Pure Cultures ◽  
Oral Candida ◽  
Almost All

Microbiological reports of apical periodontitis have revealed that yeasts can be isolated from approximately 5-20% of infected root canals. They occur either in pure cultures or together with bacteria. Almost all isolated yeasts belong to the genus Candida, and the predominant species is C. albicans. Pheno- and genotypic profiles of C. albicans isolates show heterogeneity comparable with those of isolates from other oral sites. C. albicans expresses several virulence factors that are capable of infecting the dentin-pulp complex, including dentinal tubules. This causes, consequentially, an inflammatory response around the root apex, which suggests a pathogenic role for this organism in apical periodontitis. Yeasts are particularly associated with persistent root canal infections that do not respond favorably to conservative root canal therapy. This may be due to the resistance of all oral Candida species against a commonly used topical medicament, calcium hydroxide. However, other antimicrobial agents may offer alternative therapeutic approaches and improve the treatment of these persistent cases of apical periodontitis.

scholarly journals Microbial Profile of Root Canals of Pulpally Infected Teeth In Ghanaians

2020 ◽  
Author(s):  
Akua Boakyewaa Konadu ◽  
Ebenezer Anno Nyako ◽  
Patrick Caldicock Ampofo ◽  
Moses Lorenzo Akyeh ◽  
Thomas Akuetteh Ndanu ◽  
...  
Keyword(s):  
Root Canal ◽  
Bacterial Species ◽  
Primary Root ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Facultative Anaerobes ◽  
Microbial Profile ◽  
Laboratory Setup ◽  
Infected Root ◽  
Micro Organisms

Abstract Background Pulpal and periapical infections are initiated by microorganisms when they gain access into the dental pulp. The success of root canal treatment principally depends on the eradication of the micro-organisms in the root canal system. The aim of the study was to determine the microbial agents of infected root canals in Ghanaians patients. Methodology Forty four consecutive patients with sixty teeth referred to the Restorative Dentistry Clinic requiring root canal treatment were recruited. Root canal samples were collected from the teeth with sterile paper points. The samples were processed at the laboratory setup created at the chairside, subjected for microbial analysis and identification using Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS).ResultsA total of 259 isolates were recovered from the 60 infected root canals, belonging to twenty (20) different microbial genera. Out of the 259 microbial species isolated, only two (2) were candida albicans, a fungi; 257(99.2%) were bacterial isolates belonging to 19 genera. The 19 genera had 53 bacterial species, out of which 26(49.1%) were identified as facultative anaerobes, 15(28.3 %.) as obligate anaerobes and 12(22.6%) were aerobes. Streptococcus species (Streptococcus oralis, S.mitis, S. mutans and S. constellatus) were the most predominant isolates, followed by Prevotella sp, Actinomyces sp, Enterococcus faecalis and Rothia sp respectively. ConclusionThe findings of this study show that, primary root canal infections are polymicrobial with facultative anaerobes been predominant. The determination of the microbial profile aids in understanding the pathogenesis of pulpal and periradicular infections and help in choosing effective antimicrobial irrigation and medicament for root canal treatment.

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