ANTIBIOTIC-RESISTANT ESCHERICHIA COLI CAUSING URINARY-TRACT INFECTION IN GENERAL PRACTICE: RELATION TO FÆCAL FLORA

Escherichia coli (E. coli) is the most common type of pathogen that causes Urinary tract infection disease. It can be presented as a pathogenic or non-pathogenic strain and found not only in the animal but also in the human intestine. This bacterium can cause opportunistic infection when the human host comprised of thalassemia patients or changes the healthy hemostatic flora. This study aimed to analyze the presence of bacteria in thalassemia patients with urinary tract infection. A total of 303 samples were collected during the period from August 2019 to January 2020 from thalassemia patients who suffered from urinary tract infection. The results showed that there were 6.9% of patients infected with E. coli, 2.6% of patients were infected with S. aureus, 0.7% with both Proteus and Klebsiella, while 89.1% of patients had a negative sample for bacteria. Also, the incidence of urinary tract infections in females is higher than in males. Besides, its occurrence in rural areas is higher than in city residents. Moreover, among 16 antibiotics tested to sensitize bacteria to antibiotics, Imipenem showed 100% efficacy on all isolated bacteria. In contrast, Netilmicin showed 80.1% efficacy, Gentamycin 80.1%, and Amikacin 76.2%. Ampicillin, Aztreonam, Amoxicillin-Clavulanic Acid, Tetracycline, and Ticarcillin-Clavulanic Acid, did not show any effectiveness toward the bacteria while other antibiotics showed different activities. Furthermore, the isolated microbes from thalassemia patients were the highest resistance to antibiotics in comparison with other studies, and this antibiotic-resistant may be due to the weakening of the patient's immune status and frequent blood taking and the antibodies it contains.

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Rapid detection of antibiotic-resistant Escherichia coli in livestock with urinary tract infection using third-generation sequencing

10.26226/morressier.5f4ccc57648d3d8b3f362bf5 ◽

2020 ◽

Author(s):

Larionova Olga ◽

Zaytsev Sergey ◽

Khizhnyakova Mariya ◽

Feodorova Valentina Anatolievna

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Rapid Detection ◽

Third Generation ◽

Antibiotic Resistant ◽

Third Generation Sequencing ◽

Generation Sequencing

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Relationship between Escherichia coli strains causing urinary tract infection in women and the dominant faecal flora of the same hosts

Epidemiology and Infection ◽

10.1017/s0950268806005917 ◽

2006 ◽

Vol 134 (5) ◽

pp. 1015-1023 ◽

Cited By ~ 48

Author(s):

E. MORENO ◽

A. ANDREU ◽

T. PÉREZ ◽

M. SABATÉ ◽

J. R. JOHNSON ◽

...

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Virulence Genes ◽

Virulence Gene ◽

Faecal Flora ◽

E Coli ◽

Virulent Strains ◽

Faecal Samples

To clarify whether prevalence or special pathogenicity is more important in determining urinary tract infection (UTI) causation, we compared the biotype, phylogenetic group, and virulence genes of Escherichia coli urine strains from 11 women with acute lower UTI with those of the host's dominant intestinal E. coli strain(s). Twenty-one unique E. coli clones were identified. For three women, the single faecal clone identified was also the host's urine clone, whereas for eight women faecal samples yielded 1 or 2 distinct non-urine clones (total, n=10), either with (n=3) or without (n=5) the concurrent urine clone. The eight urine clones from the latter eight women exhibited significantly greater inferred virulence, according to virulence gene content and phylogenetic background, than did the hosts' 10 corresponding ‘faecal only’ clones. In contrast, the three urine clones that were detected as the host's sole faecal clone exhibited significantly lower inferred virulence than the other eight urine clones, and were statistically indistinguishable from the 10 ‘faecal only’ clones. In conclusion, special pathogenicity is an important determinant of UTI pathogenesis in women, although prevalence may occasionally allow less virulent strains to cause UTI.

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Antibiotic Susceptibility of Escherichia Coli Isolate from Urinary Tract Infection Of Thalassemic Patients in Thi-Qar Province

Southern Brazilian Journal of Chemistry - Southern Brazilian Journal of Chemistry, Volume 28, No. 28, 2020 ◽

10.37633/sbjc.28(28)2020.22-33 ◽

2020 ◽

Vol 28 (28) ◽

pp. 22-33

Author(s):

Alaa Abood Yasir Okab ◽

Manal Salih

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Clavulanic Acid ◽

Rural Areas ◽

Antibiotic Resistant ◽

E Coli ◽

Amoxicillin Clavulanic Acid ◽

Tract Infections

Escherichia coli (E. coli) is the most common type of pathogen that causes Urinary tract infection disease. It can be presented as pathogenic or non-pathogenic strain and found not only in the animal but also in the human intestine. This bacterium can cause opportunistic infection when the human host comprised of thalassemia patients or changes the healthy hemostatic flora. This study aimed to analyze the presence of bacteria in thalassemia patients with urinary tract infection. A total of 303 samples were collected during the period from August 2019 to January 2020 from thalassemia patients who suffered from urinary tract infection. The results showed that there were 6.9% of patients infected with E. coli, 2.6% of patients were infected with S. aureus, 0.7% with both Proteus and Klebsiella, while 89.1% of patients had a negative sample for bacteria. Also, the incidence of urinary tract infections in females is higher than in males. Besides, its occurrence in rural areas is higher than in city residents. Moreover, among 16 antibiotics tested to sensitize bacteria to antibiotics, Imipenem showed 100% efficacy on all isolated bacteria. In contrast, Netilmicin showed 80.1% efficacy, Gentamycin 80.1%, and Amikacin 76.2%. Ampicillin, Aztreonam, Amoxicillin-Clavulanic Acid, Tetracycline, and Ticarcillin-Clavulanic Acid, did not show any effectiveness toward the bacteria while other antibiotics showed different activities. Furthermore, the isolated microbes from thalassemia patients were the highest resistance to antibiotics in comparison with other studies, and this antibiotic-resistant may be due to the weakening of the patient's immune status and frequent blood taking and the antibodies it contains.

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Faecal Escherichia coli from patients with E. coli urinary tract infection and healthy controls who have never had a urinary tract infection

Journal of Medical Microbiology ◽

10.1099/jmm.0.068783-0 ◽

2014 ◽

Vol 63 (4) ◽

pp. 582-589 ◽

Cited By ~ 34

Author(s):

Karen L. Nielsen ◽

Pia Dynesen ◽

Preben Larsen ◽

Niels Frimodt-Møller

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Multidrug Resistance ◽

Urinary Tract ◽

Tract Infection ◽

Control Group ◽

Healthy Controls ◽

Faecal Flora ◽

E Coli ◽

Tract Infections

Urinary tract infections (UTIs) are primarily caused by Escherichia coli with the patient’s own faecal flora acting as a reservoir for the infecting E. coli. Here we sought to characterize the E. coli faecal flora of UTI patients and healthy controls who had never had a UTI. Up to 20 E. coli colonies from each rectal swab were random amplified polymorphic DNA (RAPD) typed for clonality, dominance in the sample and correlation to the infecting UTI isolate in patients. Each distinct clone was phylotyped and tested for antimicrobial susceptibility. Eighty-seven per cent of the UTI patients carried the infecting strain in their faecal flora, and faecal clones causing UTI were more often dominant in the faecal flora. Patients had a larger diversity of E. coli in their gut flora by carrying more unique E. coli clones compared to controls, and patient faecal clones were more often associated with multidrug resistance compared to controls. We found a similar phylotype distribution of faecal clones from UTI patients and healthy controls, including a large proportion of B2 isolates in the control group. Faecal-UTI isolates from patients were more often associated with multidrug resistance compared to faecal-only clones, indicating a link between UTI virulence and antimicrobial resistance. Intake of any antibiotic less than 6 months prior to inclusion in the experiment occurred significantly more in patients with UTI than in controls. In contrast, presence of an intrauterine device was significantly more common in controls indicating a protective effect against UTI. In conclusion, healthy controls have a large proportion of potentially pathogenic E. coli phylotypes in their faecal flora without this causing infection.

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Community-Acquired Urinary Tract Infection byEscherichia coliin the Era of Antibiotic Resistance

BioMed Research International ◽

10.1155/2018/7656752 ◽

2018 ◽

Vol 2018 ◽

pp. 1-14 ◽

Cited By ~ 20

Author(s):

Dong Sup Lee ◽

Seung-Ju Lee ◽

Hyun-Sop Choe

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Antibiotic Resistance ◽

Urinary Tract ◽

Tract Infection ◽

Urinary Tract Infections ◽

Oral Antibiotics ◽

Antibiotic Resistant ◽

E Coli ◽

Tract Infections

Urinary tract infections (UTIs) caused byEscherichia coli (E. coli)are the most common types of infections in women. The antibiotic resistance ofE. coliis increasing rapidly, causing physicians to hesitate when selecting oral antibiotics. In this review, our objective is to ensure that clinicians understand the current seriousness of antibiotic-resistantE. coli, the mechanisms by which resistance is selected for, and methods that can be used to prevent antibiotic resistance.

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