Tissue engineering of white adipose tissue using hyaluronic acid-based scaffolds. I: in vitro differentiation of human adipocyte precursor cells on scaffolds

Biomaterials ◽  
2003 ◽  
Vol 24 (18) ◽  
pp. 3125-3132 ◽  
Author(s):  
Melanie Halbleib ◽  
Thomas Skurk ◽  
Claudio de Luca ◽  
Dennis von Heimburg ◽  
Hans Hauner
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Hyaluronic Acid ◽  
White Adipose Tissue ◽  
Precursor Cells ◽  
In Vitro Differentiation ◽  
Human Adipocyte ◽  
Adipocyte Precursor

Relationship between replication and differentiation in cultured human adipocyte precursor cells

1996 ◽  
Vol 270 (4) ◽  
pp. C1011-C1016 ◽  
Author(s):  
G. Entenmann ◽  
H. Hauner
Keyword(s):  
Human Adipose Tissue ◽  
Significant Degree ◽  
Precursor Cells ◽  
Cell Counting ◽  
Human Adipocyte ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Adipocyte Development ◽  
Adipocyte Precursor

The aim of this study was to investigate the role of cell replication for the differentiation of human adipocyte precursor cells in primary culture. When cells were seeded in a medium supplemented with 10% fetal bovine serum, they started to proliferate within 48 h after exposure, as assessed by cell counting and [3H]thymidine autoradiography. When cells were inoculated in the absence of serum, a significant degree of cell proliferation was not detectable. Histochemical investigations using bromodeoxyuridine incorporation demonstrated that cells replicating their DNA did not accumulate lipid droplets. Inoculating adipocyte precursor cells under completely serum-free conditions resulted in a 30-50% higher expression of lipogenic enzymes such as glycerol-3-phosphate dehydrogenase and lipoprotein lipase than keeping cells in serum-supplemented medium for the initial 16 h. Addition of cytosine arabinoside at concentrations that effectively block mitosis did not interfere with adipocyte development. In conclusion, adipocyte precursor cells from human adipose tissue do not require cell division to enter the differentiation process in vitro. These cells may have already undergone possibly critical cell divisions in vivo and may be in a late stage of adipocyte development.

Effects of transforming growth factor-α on chicken adipocyte precursor cells in vitro

1992 ◽  
Vol 134 (2) ◽  
pp. 163-168 ◽  
Author(s):  
S. C. Butterwith ◽  
C. D. Peddie ◽  
C. Goddard
Keyword(s):  
Adipose Tissue ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Transforming Growth Factor ◽  
Precursor Cells ◽  
Transforming Growth Factor Α ◽  
Igf I ◽  
Factor Α ◽  
Adipocyte Precursor

ABSTRACT The hyperplastic capacity of adipose tissue resides in a group of fibroblast-like adipocyte precursor cells. There is evidence to suggest that their proliferation and differentiation is regulated by insulin-like growth factor-I (IGF-I) and transforming growth factor-β (TGF-β) but there is less information about other growth factors which may also participate in adipocyte precursor cell hyperplasia. Transforming growth factor-α (TGF-α) is a 50 amino acid polypeptide which has been shown to stimulate proliferation in both neoplastic and normal cell types acting through the epidermal growth factor (EGF) receptor. We have studied the regulation of DNA synthesis and the activity of lipoprotein lipase by TGF-α in chicken adipocyte precursor cells in vitro. Both TGF-α and EGF stimulated incorporation of [3H]thymidine into DNA in a dose-dependent manner. TGF-α was approximately 180-fold more potent than EGF. Addition of TGF-α in combination with IGF-I, TGF-β1 or platelet-derived growth factor produced a synergistic increase in DNA synthesis. Short-term incubation with TGF-α reduced lipoprotein lipase activity by 23%. These results show that TGF-α is a potent mitogen in these adipocyte precursor cells and can inhibit their differentiation in vitro and may participate in the regulation of adipose tissue development in vivo. Journal of Endocrinology (1992) 134, 163–168

scholarly journals Derivation, characterization, and in vitro cell regeneration of canine white adipose tissue-derived mesenchymal stem cells obtained from a mesenteric region

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Anirban Mandal ◽  
Ajeet Kumar Jha ◽  
Dew Biswas ◽  
Shyamal Kanti Guha
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Cell Regeneration ◽  
Wound Healing Assay ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background The study was conducted to assess the characterization, differentiation, and in vitro cell regeneration potential of canine mesenteric white adipose tissue-derived mesenchymal stem cells (AD-MSCs). The tissue was harvested through surgical incision and digested with collagenase to obtain a stromal vascular fraction. Mesenchymal stem cells isolated from the stromal vascular fraction were characterized through flow cytometry and reverse transcription-polymerase chain reaction. Assessment of cell viability, in vitro cell regeneration, and cell senescence were carried out through MTT assay, wound healing assay, and β-galactosidase assay, respectively. To ascertain the trilineage differentiation potential, MSCs were stained with alizarin red for osteocytes, alcian blue for chondrocytes, and oil o red for adipocytes. In addition, differentiated cells were characterized through a reverse transcription-polymerase chain reaction. Results We observed the elongated, spindle-shaped, and fibroblast-like appearance of cells after 72 h of initial culture. Flow cytometry results showed positive expression for CD44, CD90, and negative expression for CD45 surface markers. Population doubling time was found 18–24 h for up to the fourth passage and 30±0.5 h for the fifth passage. A wound-healing assay was used to determine cell migration rate which was found 136.9 ± 4.7 μm/h. We observed long-term in vitro cell proliferation resulted in MSC senescence. Furthermore, we also found that the isolated cells were capable of differentiating into osteogenic, chondrogenic, and adipogenic lineages. Conclusions Mesenteric white adipose tissue was found to be a potential source for isolation, characterization, and differentiation of MSCs. This study might be helpful for resolving the problems regarding the paucity of information concerning the basic biology of stem cells. The large-scale use of AD-MSCs might be a remedial measure in regenerative medicine.

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation
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