Induction of cellular immunity against Mycobacterium tuberculosis

1997 ◽  
Vol 56 (1-3) ◽  
pp. 446
Author(s):  
D da Fonseca
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cellular Immunity

Tuberculosis

Chest Imaging ◽  
2019 ◽  
pp. 215-219
Author(s):  
Sonia L. Betancourt
Keyword(s):  
Developing Countries ◽  
Mycobacterium Tuberculosis ◽  
Cellular Immunity ◽  
Primary Infection ◽  
Airborne Infection ◽  
Radiologic Findings ◽  
Obligate Aerobe ◽  
Spread Of Infection ◽  
Immunocompetent Patients ◽  
Increased Susceptibility

Tuberculosis (TB) is an airborne infection caused by Mycobacterium tuberculosis, an obligate aerobe, nonmotile, non-spore-forming bacillus. TB is a major cause of morbidity and mortality especially in developing countries. Patients with impaired cellular immunity including HIV (+), elderly, prisoners, and indigents and homeless patients have an increased susceptibility for active TB disease. Primary infection usually resolves without complications in immunocompetent patients. The most common radiologic manifestations of primary TB in children are consolidation and lymphadenopathy. Progressive primary TB with widespread hamatogenous dissemination may rarely occur. Patchy or nodular opacities in the upper lobes with associated cavitation are characteristic radiologic findings of active infection. While this pattern often correlates with relatively spared immunity, it denotes highly infectious individuals who require isolation.CT is very sensitive for the detection of bronchogenic spread of infection manifesting with discrete nodules, tree-in-bud opacities and cavitation.Miliary TB refers to hematogenous spread of infection.

scholarly journals Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51

2004 ◽  
Vol 72 (4) ◽  
pp. 2014-2021 ◽  
Author(s):  
Keita Miki ◽  
Toshi Nagata ◽  
Takao Tanaka ◽  
Yeung-Hyen Kim ◽  
Masato Uchijima ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Listeria Monocytogenes ◽  
Cellular Immunity ◽  
Protective Antigen ◽  
Mycobacterial Infection ◽  
Eukaryotic Expression ◽  
Recombinant Bacteria ◽  
Antigen 85 ◽  
Expression Plasmids ◽  
Mycobacterial Protein

ABSTRACT We report here the induction of specific protective cellular immunity against Mycobacterium tuberculosis by the employment of vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes Δ2 strains carrying eukaryotic expression plasmids for the antigen 85 complex (Ag85A and Ag85B) and for MPB/MPT51 (mycobacterial protein secreted by M. bovis BCG/mycobacterial protein secreted by M. tuberculosis) molecules. Infection of these recombinant bacteria allowed expression of the genes in the J774A.1 murine macrophage cell line. Intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative-specific cellular immune responses, such as foot pad reactions, proliferative responses of splenocytes, and gamma interferon production from splenocytes, suggesting the efficacy of vaccination against mycobacterial infection by use of these recombinant L. monocytogenes strains. Furthermore, intravenous vaccination with recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine. Notably, this is the first report to show that MPB/MPT51 is a major protective antigen in addition to Ag85A and Ag85B, which have been reported to be major mycobacterial protective antigens.

Induction of cellular immunity against Mycobacterium tuberculosis

1997 ◽  
Vol 56 ◽  
pp. 446
Author(s):  
D.P.A.J. da Fonseca ◽  
A.F.M. Verheul ◽  
H. Snippe
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cellular Immunity

scholarly journals Nanoscale Peptide Self-assemblies Boost BCG-primed Cellular Immunity Against Mycobacterium tuberculosis

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Charles B. Chesson ◽  
Matthew Huante ◽  
Rebecca J. Nusbaum ◽  
Aida G. Walker ◽  
Tara M. Clover ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cellular Immunity

scholarly journals Disseminated tuberculosis in interferon gamma gene-disrupted mice.

1993 ◽  
Vol 178 (6) ◽  
pp. 2243-2247 ◽  
Author(s):  
A M Cooper ◽  
D K Dalton ◽  
T A Stewart ◽  
J P Griffin ◽  
D G Russell ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Interferon Gamma ◽  
Cellular Immunity ◽  
Protective Immunity ◽  
Essential Role ◽  
Mononuclear Phagocytes ◽  
Sublethal Dose ◽  
Tissue Destruction ◽  
Ifn Gamma ◽  
Very High

The expression of protective immunity to Mycobacterium tuberculosis in mice is mediated by T lymphocytes that secrete cytokines. These molecules then mediate a variety of roles, including the activation of parasitized host macrophages, and the recruitment of other mononuclear phagocytes to the site of the infection in order to initiate granuloma formation. Among these cytokines, interferon gamma (IFN-gamma) is believed to play a key role is these events. In confirmation of this hypothesis, we show in this study that mice in which the IFN-gamma gene has been disrupted were unable to contain or control a normally sublethal dose of M. tuberculosis, delivered either intravenously or aerogenically. In such mice, a progressive and widespread tissue destruction and necrosis, associated with very high numbers of acid-fast bacilli, was observed. In contrast, despite the lack of protective immunity, some DTH-like reactivity could still be elicited. These data, therefore, indicate that although IFN-gamma may not be needed for DTH expression, it plays a pivotal and essential role in protective cellular immunity to tuberculosis infection.

scholarly journals Research progress on immune response of B lymphocytes and anti-Mycobacterium tuberculosis infection

2016 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
Jiacong You
Keyword(s):  
Immune Response ◽  
Mycobacterium Tuberculosis ◽  
B Lymphocytes ◽  
Host Defense ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Research Progress ◽  
Mycobacterium Tuberculosis Infection ◽  
Cellular Immune

Abstract Multiple studies elucidated the importance of cellular immune mechanisms for protection against Mycobacterium tuberculosis. However, recent studies showed that B lymphocytes play a role that is underestimated through various interactions with cellular immune response, forming an important aspect of host defense against M. tuberculosis bacteria. Therefore, the author hereby proposes a progressive perspective for immunology of tuberculosis, i.e., cellular immunity and humoral immunity are not necessarily mutually exclusive. The present study summarizes recent studies that support the important role of B lymphocytes in terms of M. tuberculosis infection.

scholarly journals Crossprotection against nontuberculous mycobacterial infections by Mycobacterium tuberculosis memory immune T lymphocytes.

1986 ◽  
Vol 163 (1) ◽  
pp. 203-208 ◽  
Author(s):  
I M Orme ◽  
F M Collins
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
T Lymphocytes ◽  
Cellular Immunity ◽  
Nontuberculous Mycobacteria ◽  
Antigenic Determinants ◽  
Mycobacterial Infections ◽  
Enhanced Resistance ◽  
Specific Memory

Adoptive immunization of T cell-deficient recipient mice with M. tuberculosis-specific memory immune T lymphocytes conferred upon these animals the ability to express significantly enhanced resistance both to the homologous infection, and to three strains of nontuberculous mycobacteria. These results support the hypothesis, therefore, that antigenic determinants possessed by the four mycobacterial strains that are relevant to the generation of protective cellular immunity are identical or closely crossreactive.

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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