Exercise-induced regulation of brain-derived neurotrophic factor (BDNF) transcripts in the rat hippocampus

Background: The molecular pathogenesis of depression and psychopharmacology of antidepressants remain elusive. Recent hypotheses suggest that changes in neurogenesis and plasticity may underlie the aetiology of depression. The hippocampus is affected by depression and shows neuronal remodelling during adulthood.Objective: The present study on the adult rat hippocampus, was to evaluate the dose-related effects of chronic venlafaxine on the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated cyclic-AMP response element binding protein (pCREB).Methods: Sprague-Dawley rats were exposed to a variety of chronic unpredictable stressors (CUSs) to establish a depression model. Rats were treated for either 14 or 28 days with venlafaxine (5 and 10 mg/kg, respectively). The hippocampal expression of pCREB and BDNF mRNA and protein was assessed by using immunohistochemistry, western blotting and reverse transcription polymerase chain reaction (RT-PCR).Results: Rats subjected to CUS procedure consumed less sucrose solution compared with non-stressed rats. The CUS influenced exploratory activity resulting in a reduction of the motility counts. Chronic low dose (5 mg/kg, 14 and 28 days), but not high dose (10 mg/kg, 14 and 28 days) of venlafaxine treatment increased the expression of pCREB and BDNF mRNA and protein in the CUS rat hippocampus.Conclusion: Neuronal plasticity-associated proteins such as pCREB and BDNF play an important role both in stress-related depression and in antidepressant effect.

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Brain-Derived Neurotrophic Factor Enhances Bcl-xL Expression Through Protein Kinase Casein Kinase 2-Activated and Nuclear Factor Kappa B-Mediated Pathway in Rat Hippocampus

Brain Pathology ◽

10.1111/j.1750-3639.2010.00431.x ◽

2011 ◽

Vol 21 (2) ◽

pp. 150-162 ◽

Cited By ~ 26

Author(s):

Chih C. Chao ◽

Yun L. Ma ◽

Eminy H.Y. Lee

Keyword(s):

Protein Kinase ◽

Neurotrophic Factor ◽

Nuclear Factor Kappa B ◽

Brain Derived Neurotrophic Factor ◽

Casein Kinase ◽

Casein Kinase 2 ◽

Rat Hippocampus ◽

Nuclear Factor ◽

Nuclear Factor Kappa

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The influence of vigorous aerobic exercise on serum brain-derived neurotrophic factor and estradiol in young, healthy women as a function of the menstrual cycle

10.31234/osf.io/hwegb ◽

2021 ◽

Author(s):

Sarah Ahmad ◽

Rodney Hansen ◽

Matthew Schmolesky

Keyword(s):

Menstrual Cycle ◽

Aerobic Exercise ◽

Neurotrophic Factor ◽

Brain Derived Neurotrophic Factor ◽

Cycle Exercise ◽

Acute Bout ◽

Healthy Women ◽

Exercise Induced ◽

Induced Changes ◽

Serum Bdnf

AbstractResearch suggests strong inter-relationships between physical exercise, levels of brain-derived neurotrophic factor (BDNF), levels of estrogen, and the menstrual cycle, and yet no single study has examined these factors collectively in humans. The current study assessed the effect of an acute bout of vigorous aerobic exercise (20 minutes of stationary cycling at 80% of heart rate reserve) on serum BDNF and estradiol in healthy, eumenorrheic women, ages 18-28. In addition, this study determined whether basal BDNF or the exercise-induced increase in BDNF varies throughout the menstrual cycle. Thirty-four subjects were assigned to an experimental (n = 27) or control condition (n = 7). Exercise transiently increased both estradiol (51.2%) and BDNF (23.6%), and basal levels of BDNF and estradiol predicted the magnitude of the exercise-induced increases. Basal BDNF did not vary significantly throughout the menstrual cycle. Exercise-induced changes in BDNF did not correlate with menstrual cycle day or basal estradiol. Basal estradiol and basal BDNF showed a marginally significant positive correlation. Taken together, these results indicate that brief, vigorous aerobic exercise is sufficient to elevate both BDNF and estradiol in healthy women and that the menstrual cycle dramatically influences the magnitude of exercise-induced changes in estradiol, but not BDNF

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