Characterization of Non-Lymphoid Cells in Peyer’s Patches of the Rat

Immunobiology ◽  
1983 ◽  
Vol 164 (2) ◽  
pp. 136-143 ◽  
Author(s):  
T. Sminia ◽  
M.M. Wilders ◽  
E.M. Janse ◽  
E.C.M. Hoefsmit
Keyword(s):  
Lymphoid Cells ◽  
Peyer's Patches ◽  
Peyer’S Patches

Phenotypic and functional characterization of CD11c+ dendritic cell population in mouse Peyer's patches

1996 ◽  
Vol 26 (8) ◽  
pp. 1801-1806 ◽  
Author(s):  
Christiane Ruedl ◽  
Claudia Rieser ◽  
Günther Böck ◽  
Georg Wick ◽  
Hugo Wolf
Keyword(s):  
Dendritic Cell ◽  
Cell Population ◽  
Functional Characterization ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Dendritic Cell Population

scholarly journals Mucin-Related Epitopes Distinguish M Cells and Enterocytes in Rabbit Appendix and Peyer’s Patches

1999 ◽  
Vol 67 (1) ◽  
pp. 357-367 ◽  
Author(s):  
Hugues Lelouard ◽  
Hubert Reggio ◽  
Paul Mangeat ◽  
Marian Neutra ◽  
Philippe Montcourrier
Keyword(s):  
Epithelial Cells ◽  
Plasma Membranes ◽  
Secretory Granules ◽  
Lymphoid Cells ◽  
Peyer's Patches ◽  
Lymphoid Tissues ◽  
Peyer’S Patches ◽  
M Cells ◽  
Apical Membranes ◽  
Rabbit Appendix

ABSTRACT The biochemical composition of the apical membranes of epithelial M cells overlying the gut-associated lymphoid tissues (GALT) is still largely unknown. We have prepared monoclonal antibodies (MAbs) directed against carbonate-washed plasma membranes from epithelial cells detached with EDTA from rabbit appendix, a tissue particularly rich in GALT. As determined by immunofluorescence microscopy, several MAbs specifically recognized either M cells or enterocyte-like cells of the domes from rabbit appendix, sacculus rotundus, and Peyer’s patches. M cells were identified by their large ventral pocket containing lymphoid cells and by specific labeling with antivimentin. Among various characterized MAbs, MAb 104 recognized rabbit immunoglobulins and was used as an apical marker for M cells in the rabbit appendix, MAb 58 selectively stained an integral membrane glycoprotein of greater than 205 kDa located at the apex of M cells, and MAb 214 stained a smaller soluble glycoprotein associated with the apical surfaces from neighboring enterocytes. In addition, both MAbs 58 and 214 also labeled luminal mucus and secretory granules in goblet cells. The selective association of mucin-related molecules at the surfaces of either M cells or enterocyte-like cells of the follicle-associated epithelium suggests that specific carbohydrate antigens are differentially expressed by epithelial cells and could account for the differential binding properties of pathogens.

Definition of Dendritic Cell Subpopulations Present in the Spleen, Peyer’s Patches, Lymph Nodes, and Skin of the Mouse

Blood ◽  
1999 ◽  
Vol 93 (2) ◽  
pp. 590-598 ◽  
Author(s):  
Fabienne Anjuère ◽  
Pilar Martı́n ◽  
Isabel Ferrero ◽  
Marta López Fraga ◽  
Gloria Martı́nez del Hoyo ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Peyer's Patches ◽  
Microbial Pathogens ◽  
Peyer’S Patches ◽  
Leukocyte Function ◽  
Essential Function ◽  
Cell Subpopulations ◽  
Definition Of ◽  
Antigen Presenting

Dendritic cells (DC) are highly efficient antigen-presenting cells (APC) that have an essential function in the development of immune responses against microbial pathogens and tumors. Although during the past few years our understanding of DC biology has remarkably increased, a precise characterization of the different DC subpopulations remains to be achieved with regard to their phenotype and lineage relationships. In this report, we have extensively studied the DC subpopulations present in the thymus, spleen, Peyer’s patches, lymph nodes (LN) and skin of the mouse. Thymus DC and 60% spleen DC have a lymphoid DC phenotype, ie, CD8+DEC-205high Mac-1low, whereas 40% spleen DC have a myeloid DC phenotype, ie, CD8−DEC-205low Mac-1high. Both CD8+and CD8− DC are leukocyte function-associated antigen-1 (LFA-1)high and highly adherent. Within Peyer’s patches the majority of DC correspond to the CD8+DEC-205high Mac-1lowlymphoid category. In the LN, together with CD8+ and CD8− DC, an additional nonadherent CD8intLFA-1int subpopulation with lymphoid DC characteristics is described. Finally, in the skin both epidermal Langerhans cells (LC) and dermal DC are CD8−DEC-205high Mac-1high , and do not express LFA-1. Interestingly, LC migration experiments indicate that LC underwent the upregulation of CD8 and LFA-1 upon migration to the LN, supporting the hypothesis that LC belong to the CD8+ lymphoid lineage.

Selective association and transport of Campylobacter jejuni through M cells of rabbit Peyer's patches

1988 ◽  
Vol 34 (10) ◽  
pp. 1142-1147 ◽  
Author(s):  
Richard I. Walker ◽  
Elsa A. Schmauder-Chock ◽  
Joe L. Parker ◽  
Donald Burr
Keyword(s):  
Electron Microscopy ◽  
Campylobacter Jejuni ◽  
Lymphoid Cells ◽  
Peyer's Patches ◽  
Adult Rabbit ◽  
Peyer’S Patches ◽  
M Cells ◽  
M Cell ◽  
Systemic Spread ◽  
Cell Follicle

M cells in the Peyer's patches may facilitate transport of pathogens such as Campylobacter jejuni from the intestine. We evaluated this hypothesis by using electron microscopy to examine Peyer's patches in ligated adult rabbit ileal loops inoculated with 5-mL suspensions of 109 cfu/mL of Campylobacter jejuni. Peyer's patches taken at intervals from 15 min to 2 h after inoculation of loops in anaesthetized rabbits provided evidence that Campylobacter jejuni selectively adhered to M cells as opposed to absorptive epithelial cells and was transported, apparently intact, into the M cell follicle. Although intercellular organisms were seen within the follicle, many others were phagocytosed by lymphoid cells. The proximity of the lymphatic and blood circulatory systems to the M cell follicle makes this a probable route for systemic spread of Campylobacter jejuni.

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