Serodiagnosis of Q fever by enzyme-linked immunosorbent assay (ELISA)

1987 ◽  
Vol 267 (1) ◽  
pp. 57-63 ◽  
Author(s):  
N. Schmeer ◽  
H. Krauss ◽  
D. Werth ◽  
H.G. Schiefer
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay

Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever.

1994 ◽  
Vol 32 (6) ◽  
pp. 1560-1565 ◽  
Author(s):  
I J Uhaa ◽  
D B Fishbein ◽  
J G Olson ◽  
C C Rives ◽  
D M Waag ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay

A study of the correlation between Coxiella burnetii seropositivity and abortions in sheep in Eastern and Southeastern Turkey

2016 ◽  
Author(s):  
Ayse Kilic ◽  
Hakan Kalender
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eastern Anatolia ◽  
Obligate Intracellular Bacterium ◽  
Serum Samples ◽  
Obligate Intracellular ◽  
Elisa Kit

Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. Infected animals are usually asymptomatic, but infection can cause abortion and stillbirth in ruminants. The main purpose of this study was to evaluate prevalance of Coxiella burnetii infection in aborted and nonaborted sheep serum samples in Eastern Anatolia region by using enzyme-linked immunosorbent assay (ELISA). The determine of prevalance in sheep flocks from four provinces (Elazig, Malatya, Tunceli, Bitlis) and tested for anti-C.burnetii antibody detection, by means of Chekit Q fever Elisa kit. 350 serum samples obtained from flocks belonging aborted sheep showed that a total of 56 (16%) were detected seropositivity, whereas 171 serum samples obtained from nonaborted sheep flocks in 13 of the 171 (7.60%) for C.burnetii in seropositivity were observed. Coxiellosis should be considered an important cause of sheep with abortion history and nonaborted in Elazig and neighboring provinces.

scholarly journals High Seroprevalence of Mycoplasma pneumoniae IgM in Acute Q Fever by Enzyme-Linked Immunosorbent Assay (ELISA)

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77640 ◽  
Author(s):  
Chung-Hsu Lai ◽  
Lin-Li Chang ◽  
Jiun-Nong Lin ◽  
Wei-Fang Chen ◽  
Li-Li Kuo ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Seroprevalence ◽  
Acute Q Fever

scholarly journals Enzyme-linked immunosorbent assay for diagnosis of chronic Q fever.

1988 ◽  
Vol 26 (10) ◽  
pp. 1978-1982 ◽  
Author(s):  
O Péter ◽  
G Dupuis ◽  
D Bee ◽  
R Lüthy ◽  
J Nicolet ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Q Fever

The application of an enzyme-linked immunosorbent assay or an immunofluorescent assay test leads to different estimates of seroprevalence ofCoxiella burnetiiin the population

2011 ◽  
Vol 140 (1) ◽  
pp. 36-41 ◽  
Author(s):  
G. J. BLAAUW ◽  
D. W. NOTERMANS ◽  
B. SCHIMMER ◽  
J. MEEKELENKAMP ◽  
J. H. J. REIMERINK ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunofluorescent Assay

SUMMARYThe diagnosis and epidemiological studies of Q fever depend on serology. Among the main methods employed are the enzyme-linked immunosorbent assay (ELISA) and the immunofluorescent assay test (IFAT). We show that two commercial assays representing the two methods with two different cut-off titres can lead to significant differences in diagnostic and seroprevalence estimates. This in turn emphasizes the need for a standardized gold method to compare the various assays; whether this standard is ‘in-house’ or commercially obtained.

scholarly journals Comparison of a Commercial Enzyme-Linked Immunosorbent Assay with Immunofluorescence and Complement Fixation Tests for Detection of Coxiella burnetii (Q Fever) Immunoglobulin M

2000 ◽  
Vol 38 (4) ◽  
pp. 1645-1647 ◽  
Author(s):  
Peter R. Field ◽  
Jody L. Mitchell ◽  
Avelina Santiago ◽  
David J. Dickeson ◽  
Sau-Wan Chan ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Complement Fixation ◽  
Q Fever ◽  
Fluorescent Antibody ◽  
Reference Method ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Elisa

A commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Q fever (PanBio Coxiella burnetiiimmunoglobulin M [IgM] ELISA, QFM-200) was compared to the indirect fluorescent antibody test (IFAT) for C. burnetii IgM and the complement fixation test (CFT). The ELISA demonstrated 92% agreement with the reference method (IFAT), and gave a sensitivity of 99% (69 of 70 samples) and a specificity of 88% (106 of 121). Specificity can be increased with confirmation by IFAT. CFT was found to have a specificity of 90% (107 of 119), although it was lacking in sensitivity (73%; 51 of 70). No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma (n = 6), chlamydia (n = 5), or legionella (n = 4) infections, although 2 of 5 patients with leptospirosis and 1 of 4 samples containing rheumatoid factor (RF) demonstrated positive results in the ELISA. Results indicate that the performance of the PanBio C. burnetii (Q fever) IgM ELISA (F = 187) is superior to that of CFT (F = 163), and consequently the ELISA should be a useful aid in the diagnosis of acute Q fever.

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