A study of the correlation between Coxiella burnetii seropositivity and abortions in sheep in Eastern and Southeastern Turkey

2016 ◽  
Author(s):  
Ayse Kilic ◽  
Hakan Kalender
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eastern Anatolia ◽  
Obligate Intracellular Bacterium ◽  
Serum Samples ◽  
Obligate Intracellular ◽  
Elisa Kit

Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. Infected animals are usually asymptomatic, but infection can cause abortion and stillbirth in ruminants. The main purpose of this study was to evaluate prevalance of Coxiella burnetii infection in aborted and nonaborted sheep serum samples in Eastern Anatolia region by using enzyme-linked immunosorbent assay (ELISA). The determine of prevalance in sheep flocks from four provinces (Elazig, Malatya, Tunceli, Bitlis) and tested for anti-C.burnetii antibody detection, by means of Chekit Q fever Elisa kit. 350 serum samples obtained from flocks belonging aborted sheep showed that a total of 56 (16%) were detected seropositivity, whereas 171 serum samples obtained from nonaborted sheep flocks in 13 of the 171 (7.60%) for C.burnetii in seropositivity were observed. Coxiellosis should be considered an important cause of sheep with abortion history and nonaborted in Elazig and neighboring provinces.

scholarly journals Comparison of a Commercial Enzyme-Linked Immunosorbent Assay with Immunofluorescence and Complement Fixation Tests for Detection of Coxiella burnetii (Q Fever) Immunoglobulin M

2000 ◽  
Vol 38 (4) ◽  
pp. 1645-1647 ◽  
Author(s):  
Peter R. Field ◽  
Jody L. Mitchell ◽  
Avelina Santiago ◽  
David J. Dickeson ◽  
Sau-Wan Chan ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Complement Fixation ◽  
Q Fever ◽  
Fluorescent Antibody ◽  
Reference Method ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Elisa

A commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Q fever (PanBio Coxiella burnetiiimmunoglobulin M [IgM] ELISA, QFM-200) was compared to the indirect fluorescent antibody test (IFAT) for C. burnetii IgM and the complement fixation test (CFT). The ELISA demonstrated 92% agreement with the reference method (IFAT), and gave a sensitivity of 99% (69 of 70 samples) and a specificity of 88% (106 of 121). Specificity can be increased with confirmation by IFAT. CFT was found to have a specificity of 90% (107 of 119), although it was lacking in sensitivity (73%; 51 of 70). No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma (n = 6), chlamydia (n = 5), or legionella (n = 4) infections, although 2 of 5 patients with leptospirosis and 1 of 4 samples containing rheumatoid factor (RF) demonstrated positive results in the ELISA. Results indicate that the performance of the PanBio C. burnetii (Q fever) IgM ELISA (F = 187) is superior to that of CFT (F = 163), and consequently the ELISA should be a useful aid in the diagnosis of acute Q fever.

scholarly journals Bovine Immunoglobulin E Levels of Bali Cattlesin Bangli and Nusa Penida island Bali Province, Indonesia

2019 ◽  
Vol 2 (2) ◽  
pp. 64
Author(s):  
Ni Ketut Suwiti ◽  
Luh Gde Surya Heryani ◽  
Desak Nyoman Dewi Indira Laksmi ◽  
Ni Nyoman Werdi Susari ◽  
I Nengah Kerta Besung ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Commercial Enzyme ◽  
Elisa Kit ◽  
Bali Cattle ◽  
Bovine Immunoglobulin

The aim of this research was to detect identify levels of Bovine Immunoglobulin E (BoIg.E), can be used as an indicator of response immune in bali cattle.  Eighty serum samples were collected from Nusa Penida and Bangli region. Bovine Ig.E levels was measured using a commercial Enzyme Linked Immunosorbent Assay (ELISA) Kit. The data were analysis based on differences of farming characteristics andgeographic. The result of research that, of BoIg.E level of bali cattle kept in Bangli (34.16258 ?g/ml), was higher than Nusa Penida (22.26047 ?g/ml). We conclude that there was a significant effect of differences of farming characteristics and geographic conditions.

scholarly journals Serological survey of Coxiella burnetii infections in dairy cattle, sheep, goats and zoo animals in Hungary – Short communication

2021 ◽  
Author(s):  
Attila Dobos ◽  
István Fodor ◽  
Gerda Kiss ◽  
Miklós Gyuranecz
Keyword(s):  
Dairy Cattle ◽  
Coxiella Burnetii ◽  
Large Scale ◽  
Q Fever ◽  
Small Ruminants ◽  
Human Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Ruminant Species ◽  
Zoo Animals

AbstractQ fever is a disease of high zoonotic potential, but interest in its causative agent is rather low although it causes some public health problems in Hungary. The prevalence of Q fever is highly variable by country. The main reservoirs of the disease are the same domestic ruminant species everywhere, but the epidemiological profile depends on the features of the specific reservoir. The aim of this large-scale study was to demonstrate the importance of Q fever in different species as a possible source for human infection in most regions of Hungary. A total of 851 serum samples from 44 dairy farms, 16 sheep flocks, 4 goat farms and 3 zoos located in different parts of Hungary were tested. The presence of antibodies to Coxiella burnetii was surveyed in dairy cattle (n = 547), goats (n = 71), sheep (n = 200) and zoo animals (n = 33). The animal species tested in Hungary showed different seroprevalence values of C. burnetii infection. Seropositivity by the enzyme-linked immunosorbent assay was found in 258 out of 547 (47.2%) cows and in 69 out of 271 (25.5%) small ruminants, among them in 47 out of 200 (23.5%) sheep and in 22 out of 71 (31.0%) goats. Antibodies to C. burnetii were not detected in zoo animals. Seropositivity was demonstrated in 44 out of 44 (100%) dairy cattle farms, with at least one serum sample found to be positive on each farm. The seropositivity rate of small ruminant farms was 55.0% (11 positive out of 20 tested), with 9 out of 16 (56.3%) sheep flocks and 2 out of 4 (50.0%) goat herds showing seropositivity.

scholarly journals Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus

2015 ◽  
Vol 22 (4) ◽  
pp. 389-397 ◽  
Author(s):  
Ming Yang ◽  
Satya Parida ◽  
Tim Salo ◽  
Kate Hole ◽  
Lauro Velazquez-Salinas ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Diagnostic Sensitivity ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nonstructural Proteins ◽  
Serum Samples ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACTFoot-and-mouth disease (FMD) is one of the most highly contagious and economically devastating diseases, and it severely constrains the international trade of animals. Vaccination against FMD is a key element in the control of FMD. However, vaccination of susceptible animals raises critical issues, such as the differentiation of infected animals from vaccinated animals. The current study developed a reliable and rapid test to detect antibodies against the conserved, nonstructural proteins (NSPs) of the FMD virus (FMDV) to distinguish infected animals from vaccinated animals. A monoclonal antibody (MAb) against the FMDV NSP 3B was produced. A competitive enzyme-linked immunosorbent assay (cELISA) for FMDV/NSP antibody detection was developed using a recombinant 3ABC protein as the antigen and the 3B-specific MAb. Sera collected from naive, FMDV experimentally infected, vaccinated carrier, and noncarrier animals were tested using the 3B cELISA. The diagnostic specificity was 99.4% for naive animals (cattle, pigs, and sheep) and 99.7% for vaccinated noncarrier animals. The diagnostic sensitivity was 100% for experimentally inoculated animals and 64% for vaccinated carrier animals. The performance of this 3B cELISA was compared to that of four commercial ELISA kits using a panel of serum samples established by the World Reference Laboratory for FMD at The Pirbright Institute, Pirbright, United Kingdom. The diagnostic sensitivity of the 3B cELISA for the panel of FMDV/NSP-positive bovine serum samples was 94%, which was comparable to or better than that of the commercially available NSP antibody detection kits. This 3B cELISA is a simple, reliable test to detect antibodies against FMDV nonstructural proteins.

scholarly journals Q fever

2012 ◽  
Vol 33 (4) ◽  
pp. 170
Author(s):  
Robert Norton
Keyword(s):  
Coxiella Burnetii ◽  
Q Fever ◽  
Environmental Sampling ◽  
High Likelihood ◽  
Obligate Intracellular Bacterium ◽  
Wet Season ◽  
Obligate Intracellular ◽  
Chronic Q Fever ◽  
Specific Symptoms ◽  
Occupational Contact

Q fever is a zoonosis caused by the obligate intracellular bacterium Coxiella burnetii. North Queensland has some of the highest rates of Q fever notifications in Australia. The clinical diagnosis of Q fever can be difficult with non-specific symptoms. Up to 5% of cases will develop chronic Q fever with a high likelihood of endocarditis. Diagnosis is essentially by serology. In North Queensland cases have clustered in relatively new, semi-rural suburbs which lie adjacent to native bushland. Native mammals are attracted to new growth in these cleared areas, particularly after the wet season. There is little or no occupational contact with traditional sources of Q fever such as cattle. Seroprevalence studies on native mammals have shown higher levels of seropositivity in native mammals than in cattle. It is postulated that the increase in human cases seen from these areas are a direct effect of interaction between native mammals and humans. Further studies on environmental sampling is currently under way.

scholarly journals Serological Evaluation of Thin-Layer Immunoassay–Enzyme-Linked Immunosorbent Assay for Antibody Detection in Human Trichinellosis

2000 ◽  
Vol 7 (5) ◽  
pp. 810-812 ◽  
Author(s):  
Alberto Gómez-Priego ◽  
Lidia Crecencio-Rosales ◽  
Jorge-Luis de-la-Rosa
Keyword(s):  
Thin Layer ◽  
Immunosorbent Assay ◽  
Trichinella Spiralis ◽  
Field Studies ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Laboratory Equipment ◽  
Specialized Equipment ◽  
Human Trichinellosis

ABSTRACT A new immunoenzymatic test, named the thin-layer immunoassay–enzyme-linked immunosorbent assay (TIA-ELISA), was evaluated for antibody detection in human trichinellosis using excretion and secretion products prepared from Trichinella spiralis muscle larvae. Serum samples from people with positive muscle biopsies or symptoms compatible with the disease (n = 8 or 26, respectively), all reactive in enzyme-linked immunoelectrotransfer blot assay (EITB), as well as 67 serum samples from healthy, EITB-negative people, were tested in an ELISA and TIA-ELISA. TIA-ELISA was performed in polystyrene plastic petri dishes by adding dots of 10 μl each of antigen (7 μg/ml) followed by adding diluted serum and the conjugate. Finally, the substrate mixed with agar was added to develop the reaction. Enzymatic by-products were easily detected by the naked eye as defined dots. Sensitivity and specificity were 76 and 94% for ELISA, and both parameters were 91% for TIA-ELISA. The kappa correlation indices for both tests in relation to EITB were 0.73 and 0.80, respectively. The TIA-ELISA can be carried out with common laboratory equipment in 3 h and uses lower quantities of antigen than EITB and ELISA. Since TIA-ELISA is easy to perform, cheap, sensitive, and specific, the test could be an acceptable alternative to use in clinical laboratories lacking specialized equipment needed for ELISA and EITB and in field studies for antibody detection in human trichinellosis.

scholarly journals Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2

2012 ◽  
Vol 19 (9) ◽  
pp. 1480-1486 ◽  
Author(s):  
Meng Ge ◽  
Wei Luo ◽  
Daliang Jiang ◽  
Runcheng Li ◽  
Wenwei Zhao ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Immunosorbent Assay ◽  
Sandwich Elisa ◽  
Test Procedure ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Swine Industry ◽  
Elisa Kit ◽  
Porcine Circovirus 2

ABSTRACTA double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the diagnostic sensitivity, specificity, and accuracy of the assay were, respectively, 90.61, 94.02, and 91.62% compared with IIF and 94.38, 95.28, and 94.67% compared with the commercial ELISA kit. Assay of 12 PCV-free pigs over a 5-week period produced only PCV2-negative titers by all 3 methods. These results and the seroprofiles of 4 pig farms obtained by both the commercial ELISA kit and the double-antigen sandwich ELISA indicate that the sandwich ELISA is a reliable method for detection of antibodies to PCV2. Additionally, the method described here permits the use of undiluted test serum samples simultaneously loaded with horseradish peroxidase (HRP)-conjugated antigen into the test well, and the complete test procedure can be performed in less than 90 min. This double-antigen sandwich ELISA should be a useful tool to aid swine industry professionals in deciding the intervention strategies for the control of PCV2-associated diseases.

scholarly journals Phagocytosis of Apoptotic Cells Increases the Susceptibility of Macrophages to Infection with Coxiella burnetii Phase II through Down-Modulation of Nitric Oxide Production

2004 ◽  
Vol 72 (4) ◽  
pp. 2075-2080 ◽  
Author(s):  
Dario S. Zamboni ◽  
Michel Rabinovitch
Keyword(s):  
Nitric Oxide ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Bacterial Load ◽  
Peritoneal Macrophages ◽  
No Synthase ◽  
Obligate Intracellular Bacterium ◽  
Biologically Relevant ◽  
Obligate Intracellular ◽  
Synthase Inhibitor

ABSTRACT Coxiella burnetii, the agent of Q fever in humans and coxiellosis in other mammals, is an obligate intracellular bacterium which is sheltered and multiplies within typically large phagolysosome-like replicative vacuoles (LRVs). We have previously shown that, compared with fibroblasts, mouse resident peritoneal macrophages control the development of LRVs and bacterial multiplication within these vacuoles. Earlier experiments with the nitric oxide (NO) synthase inhibitor aminoguanidine (AG) revealed that the control is exerted by NO induced by the bacteria. We report here that phagocytosis of apoptotic-like, but not of aldehyde-killed, lymphocytes by the macrophages reduced the production of NO induced by the bacteria and increased the development of LRVs and, therefore, the total bacterial load in the cultures. Experiments with macrophages from mice deficient for inducible NO synthase (iNOS−/−) confirmed the involvement of NO in the control of infection, since neither apoptotic lymphocytes nor AG affected the development of LRVs in these phagocytes. Since macrophages are important for the clearance of apoptotic bodies and C. burnetii is able to induce apoptosis in human monocytes, the phenomenon shown here may be biologically relevant to the development of Q fever and coxiellosis.

Development and application of an indirect enzyme-linked immunosorbent assay using recombinant S1 for serological testing of porcine epidemic diarrhea virus

2019 ◽  
Vol 65 (5) ◽  
pp. 343-352
Author(s):  
Ying Shan ◽  
Yajie Liu ◽  
Ziqi Liu ◽  
Guowei Li ◽  
Cong Chen ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Porcine Epidemic Diarrhea Virus ◽  
Fluorescent Antibody ◽  
Area Under The Curve ◽  
Antibody Detection ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was expressed with the Bac-to-Bac system and purified by nickel-affinity chromatography. An indirect enzyme-linked immunosorbent assay based on rS1 (rS1-ELISA) was then developed and optimized by checkerboard assays with serial dilutions of antigen and serum. Serum samples from 453 domestic pigs and 42 vaccinated pigs were analyzed by the indirect fluorescent antibody (IFA) test and rS1-ELISA. Taking IFA as a gold standard, rS1-ELISA produced a high sensitivity (90.7%) and specificity (94.6%) by a receiver operating characteristic (ROC) curve. In addition, ROC analysis also revealed that rS1-ELISA was consistent with IFA (area under the curve 0.9583 ± 0.0082). This rS1-ELISA was then applied to antibody detection in inactivated PEDV vaccinated pigs. The antibody could be detected 2–4 weeks after the first inoculation. These results indicated that the rS1-ELISA established in this study provides a promising and reliable tool for serologic detection of anti-PEDV IgG antibodies in infected or vaccinated pigs.

Sign in / Sign up

Export Citation Format

Share Document