The application of an enzyme-linked immunosorbent assay or an immunofluorescent assay test leads to different estimates of seroprevalence ofCoxiella burnetiiin the population

2011 ◽  
Vol 140 (1) ◽  
pp. 36-41 ◽  
Author(s):  
G. J. BLAAUW ◽  
D. W. NOTERMANS ◽  
B. SCHIMMER ◽  
J. MEEKELENKAMP ◽  
J. H. J. REIMERINK ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunofluorescent Assay

SUMMARYThe diagnosis and epidemiological studies of Q fever depend on serology. Among the main methods employed are the enzyme-linked immunosorbent assay (ELISA) and the immunofluorescent assay test (IFAT). We show that two commercial assays representing the two methods with two different cut-off titres can lead to significant differences in diagnostic and seroprevalence estimates. This in turn emphasizes the need for a standardized gold method to compare the various assays; whether this standard is ‘in-house’ or commercially obtained.

Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever.

1994 ◽  
Vol 32 (6) ◽  
pp. 1560-1565 ◽  
Author(s):  
I J Uhaa ◽  
D B Fishbein ◽  
J G Olson ◽  
C C Rives ◽  
D M Waag ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

2004 ◽  
Vol 34 (5) ◽  
pp. 1525-1529 ◽  
Author(s):  
Cristiane Divan Baldani ◽  
Rosangela Zacarias Machado ◽  
Paulo de Tarso Landgraf Botteon ◽  
Felipe Santoro Takakura ◽  
Carlos Luiz Massard
Keyword(s):  
Immunosorbent Assay ◽  
Serological Test ◽  
Epidemiological Studies ◽  
Sao Paulo ◽  
Enzyme Linked Immunosorbent Assay ◽  
São Paulo ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Babesia Equi ◽  
Specificity And Sensitivity

A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.

A study of the correlation between Coxiella burnetii seropositivity and abortions in sheep in Eastern and Southeastern Turkey

2016 ◽  
Author(s):  
Ayse Kilic ◽  
Hakan Kalender
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eastern Anatolia ◽  
Obligate Intracellular Bacterium ◽  
Serum Samples ◽  
Obligate Intracellular ◽  
Elisa Kit

Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. Infected animals are usually asymptomatic, but infection can cause abortion and stillbirth in ruminants. The main purpose of this study was to evaluate prevalance of Coxiella burnetii infection in aborted and nonaborted sheep serum samples in Eastern Anatolia region by using enzyme-linked immunosorbent assay (ELISA). The determine of prevalance in sheep flocks from four provinces (Elazig, Malatya, Tunceli, Bitlis) and tested for anti-C.burnetii antibody detection, by means of Chekit Q fever Elisa kit. 350 serum samples obtained from flocks belonging aborted sheep showed that a total of 56 (16%) were detected seropositivity, whereas 171 serum samples obtained from nonaborted sheep flocks in 13 of the 171 (7.60%) for C.burnetii in seropositivity were observed. Coxiellosis should be considered an important cause of sheep with abortion history and nonaborted in Elazig and neighboring provinces.

scholarly journals High Seroprevalence of Mycoplasma pneumoniae IgM in Acute Q Fever by Enzyme-Linked Immunosorbent Assay (ELISA)

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77640 ◽  
Author(s):  
Chung-Hsu Lai ◽  
Lin-Li Chang ◽  
Jiun-Nong Lin ◽  
Wei-Fang Chen ◽  
Li-Li Kuo ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Seroprevalence ◽  
Acute Q Fever

scholarly journals Estimation of malaria transmission in high-risk provinces of Morocco

2003 ◽  
Vol 9 (4) ◽  
pp. 542-547
Author(s):  
C. Faraj ◽  
E. Adlaoui ◽  
M. Rhajaoui ◽  
M. Lyagoubi
Keyword(s):  
High Risk ◽  
Plasmodium Vivax ◽  
Malaria Transmission ◽  
Immunosorbent Assay ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Light Traps ◽  
Human Malaria ◽  
Mosquito Collection ◽  
Transmission Level

The malaria transmission level of Plasmodium vivax was monitored in four high-risk provinces in Morocco. Intensive mosquito collection by light traps and manual catches resulted in the capture of four species: Anopheles labranchiae, An. sergenti, An. cinereus, and An. claviger. All An. sergenti and An. labranchiae females collected were tested for the presence of two phenotypes of P. vivax [PVK210 and PVK247] antigen by enzyme-linked immunosorbent assay [ELISA]. No P. vivax antigen was detected in 1347 mosquitoes analysed. A parallel parasitological investigation was conducted. Of 2665 slides examined from a population of 4343 people for detection of P. vivax, no slide was positive. The results confirm the break in malaria transmission in residual foci. The use of ELISA is recommended in future epidemiological studies of human malaria.

scholarly journals Enzyme-linked immunosorbent assay for diagnosis of chronic Q fever.

1988 ◽  
Vol 26 (10) ◽  
pp. 1978-1982 ◽  
Author(s):  
O Péter ◽  
G Dupuis ◽  
D Bee ◽  
R Lüthy ◽  
J Nicolet ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Q Fever ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Q Fever

scholarly journals Detection of Cattle Naturally Infected with Anaplasma marginale in a Region of Endemicity by Nested PCR and a Competitive Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5

1998 ◽  
Vol 36 (3) ◽  
pp. 777-782 ◽  
Author(s):  
Susana Torioni de Echaide ◽  
Donald P. Knowles ◽  
Travis C. McGuire ◽  
Guy H. Palmer ◽  
Carlos E. Suarez ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Immunosorbent Assay ◽  
Nested Pcr ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Surface Protein ◽  
Persistent Infections ◽  
Major Surface

A competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5 (rMSP5-cELISA) of Anaplasma marginale was validated in a naturally infected cattle herd in an area of eastern Oregon where A. marginale is endemic. The true positive and negative A. marginale infection status of 235 randomly selected cattle was determined by using a nested PCR (nPCR) coupled with msp5 sequence analysis and hybridization. Judgment of the reliability of the nPCR and hybridization for detection of persistent infections was based on three observations. First, the nPCR was able to detect as few as 30 infected erythrocytes per ml. Second, the nPCR was able to consistently detect low levels of rickettsemia in seven carrier cattle experimentally infected with A. marginale. Third, msp5sequence analysis showed >95% identity among 30 nPCR amplicons from cattle naturally infected with field strains of A. marginale. The nPCR and hybridization identified 151 infected and 84 uninfected cattle among the 235 animals tested. With a cutoff point of 28%, the rMSP5-cELISA showed a sensitivity of 96% and a specificity of 95%. These results indicate that the rMSP5-cELISA can sensitively and specifically detect cattle with naturally acquired persistent A. marginale infections and suggest that it is an excellent assay for epidemiological studies, eradication programs, and regulation of international cattle movement.

scholarly journals Development of a Polyclonal Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Ehrlichia ruminantium

2003 ◽  
Vol 10 (5) ◽  
pp. 910-916 ◽  
Author(s):  
Keith J. Sumption ◽  
Edith A. Paxton ◽  
Lesley Bell-Sakyi
Keyword(s):  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
High Titer ◽  
Antibody Test ◽  
Epidemiological Studies ◽  
Diagnostic Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ehrlichia Ruminantium ◽  
Cross Reactions ◽  
Diagnostic Sensitivity And Specificity

ABSTRACT A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E. ruminantium as the antigen and biotin-labeled polyclonal goat immunoglobulins as the competitor. For goats, the diagnostic sensitivity and specificity were both 100% with a cutoff of 80% inhibition (80 PI), with detection of antibodies for 550 days postinfection. For cattle, diagnostic sensitivity and specificity were 86 and 100%, respectively, with a cutoff of 50 PI and 79 and 100% with a cutoff of 70 PI. Cross-reactions with high-titer experimental or field antisera to other Ehrlichia and Anaplasma species were observed at up to 68 PI in cattle and up to 85 PI in sheep, and therefore to exclude these cross-reactions, cutoffs of 70 PI for bovine serology and 85 PI for small-ruminant serology were selected. Application of the PC-ELISA to bovine field sera from South Africa gave a higher proportion of positive results than application of the murine macrophage immunofluorescent antibody test or indirect ELISA, suggesting a better sensitivity for detection of recovered cattle, and results with bovine field sera from Malawi were consistent with the observed endemic state of heartwater and the level of tick control practiced at the sample sites. Reproducibility was high, with average standard deviations intraplate of 1.2 PI and interplate of 0.6 PI. The test format is simple, and the test is economical to perform and has a level of sensitivity for detection of low-titer positive bovine sera that may prove to be of value in epidemiological studies on heartwater.

scholarly journals rK39 Enzyme-Linked Immunosorbent Assay for Diagnosis of Leishmania donovani Infection

1998 ◽  
Vol 5 (5) ◽  
pp. 717-720 ◽  
Author(s):  
E. E. Zijlstra ◽  
N. S. Daifalla ◽  
P. A. Kager ◽  
E. A. G. Khalil ◽  
A. M. El-Hassan ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Leishmania Donovani ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Subclinical Infection ◽  
Kala Azar ◽  
Leishmanin Skin Test ◽  
Parasite Replication ◽  
Negative Controls

ABSTRACT The rK39 enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for Leishmania donovani infection in the Sudan. rK39 ELISA proved more sensitive than DAT in diagnosis of kala-azar (93 and 80%, respectively); both tests may remain positive up to 24 months after treatment. For patients with post-kala-azar dermal leishmaniasis and individuals with subclinical infection, rK39 ELISA performed as well as DAT but could detect infection 6 months earlier in ∼40% of patients. Conversion in DAT and rK39 ELISA also occurred in leishmanin skin test (LST)-positive individuals, suggesting active parasite replication (rK39 is an amastigote antigen) in these presumably immune individuals. In contrast to DAT, rK39 ELISA also detected infection in randomly selected LST-positive individuals (in four of six) and endemicity (LST-negative) controls (in one of five). rK39 ELISA appears more sensitive than DAT and may prove an important tool in epidemiological studies.

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